"Tubulin glycylation controls axonemal dynein activity, flagellar beat and male fertility": Pre-pre-power stroke conformation of the mouse sperm outer dynein arms (ODAs). Both beta- and gamma-heavy chains are in pre-power stroke conformation. Cryo-ET, classification and sub-tomogram averaging.
マップデータ
Pre-pre-power stroke conformation of the mouse sperm outer dynein arms (ODAs). Both %u03B2- and %u03B3-heavy chains are in pre-power stroke conformation. Cryo-ET, classification and sub-tomogram averaging.
ジャーナル: Science / 年: 2021 タイトル: Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility. 著者: Sudarshan Gadadhar / Gonzalo Alvarez Viar / Jan Niklas Hansen / An Gong / Aleksandr Kostarev / Côme Ialy-Radio / Sophie Leboucher / Marjorie Whitfield / Ahmed Ziyyat / Aminata Touré / Luis ...著者: Sudarshan Gadadhar / Gonzalo Alvarez Viar / Jan Niklas Hansen / An Gong / Aleksandr Kostarev / Côme Ialy-Radio / Sophie Leboucher / Marjorie Whitfield / Ahmed Ziyyat / Aminata Touré / Luis Alvarez / Gaia Pigino / Carsten Janke / 要旨: Posttranslational modifications of the microtubule cytoskeleton have emerged as key regulators of cellular functions, and their perturbations have been linked to a growing number of human pathologies. ...Posttranslational modifications of the microtubule cytoskeleton have emerged as key regulators of cellular functions, and their perturbations have been linked to a growing number of human pathologies. Tubulin glycylation modifies microtubules specifically in cilia and flagella, but its functional and mechanistic roles remain unclear. In this study, we generated a mouse model entirely lacking tubulin glycylation. Male mice were subfertile owing to aberrant beat patterns of their sperm flagella, which impeded the straight swimming of sperm cells. Using cryo-electron tomography, we showed that lack of glycylation caused abnormal conformations of the dynein arms within sperm axonemes, providing the structural basis for the observed dysfunction. Our findings reveal the importance of microtubule glycylation for controlled flagellar beating, directional sperm swimming, and male fertility.
ダウンロード / ファイル: emd_11942.map.gz / 形式: CCP4 / 大きさ: 695.3 KB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Pre-pre-power stroke conformation of the mouse sperm outer dynein arms (ODAs). Both %u03B2- and %u03B3-heavy chains are in pre-power stroke conformation. Cryo-ET, classification and sub-tomogram averaging.
ボクセルのサイズ
X=Y=Z: 9.422 Å
密度
表面レベル
登録者による: 6.54 / ムービー #1: 6.54
最小 - 最大
-22.47517 - 32.50726
平均 (標準偏差)
1.3340255 (±7.365984)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
32
10
サイズ
60
76
39
Spacing
76
60
39
セル
A: 716.072 Å / B: 565.32 Å / C: 367.458 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
9.422
9.422
9.422
M x/y/z
76
60
39
origin x/y/z
0.000
0.000
0.000
length x/y/z
716.072
565.320
367.458
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
32
0
10
NC/NR/NS
76
60
39
D min/max/mean
-22.475
32.507
1.334
-
添付データ
-
試料の構成要素
-
全体 : axonemal outer dynein arm (ODA)
全体
名称: axonemal outer dynein arm (ODA)
要素
複合体: axonemal outer dynein arm (ODA)
-
超分子 #1: axonemal outer dynein arm (ODA)
超分子
名称: axonemal outer dynein arm (ODA) / タイプ: complex / ID: 1 / 親要素: 0 詳細: Pre-pre-power stroke conformation of the mouse sperm outer dynein arms (ODAs). Both beta- and gamma- heavy chains are in pre-power stroke conformation. Reconstructed with cryo-ET, ...詳細: Pre-pre-power stroke conformation of the mouse sperm outer dynein arms (ODAs). Both beta- and gamma- heavy chains are in pre-power stroke conformation. Reconstructed with cryo-ET, classification and sub-tomogram averaging.