Journal: Sci Adv / Year: 2021 Title: Capsid opening enables genome release of iflaviruses. Authors: Karel Škubník / Lukáš Sukeník / David Buchta / Tibor Füzik / Michaela Procházková / Jana Moravcová / Lenka Šmerdová / Antonín Přidal / Robert Vácha / Pavel Plevka / Abstract: The family Iflaviridae includes economically important viruses of the western honeybee such as deformed wing virus, slow bee paralysis virus, and sacbrood virus. Iflaviruses have nonenveloped virions ...The family Iflaviridae includes economically important viruses of the western honeybee such as deformed wing virus, slow bee paralysis virus, and sacbrood virus. Iflaviruses have nonenveloped virions and capsids organized with icosahedral symmetry. The genome release of iflaviruses can be induced in vitro by exposure to acidic pH, implying that they enter cells by endocytosis. Genome release intermediates of iflaviruses have not been structurally characterized. Here, we show that conformational changes and expansion of iflavirus RNA genomes, which are induced by acidic pH, trigger the opening of iflavirus particles. Capsids of slow bee paralysis virus and sacbrood virus crack into pieces. In contrast, capsids of deformed wing virus are more flexible and open like flowers to release their genomes. The large openings in iflavirus particles enable the fast exit of genomes from capsids, which decreases the probability of genome degradation by the RNases present in endosomes.
History
Deposition
Sep 9, 2020
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Header (metadata) release
Feb 10, 2021
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Map release
Feb 10, 2021
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Update
Feb 10, 2021
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Current status
Feb 10, 2021
Processing site: PDBe / Status: Released
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