Journal: EMBO J / Year: 2005 Title: Maturation of phage T7 involves structural modification of both shell and inner core components. Authors: Xabier Agirrezabala / Jaime Martín-Benito / José R Castón / Roberto Miranda / José María Valpuesta / José L Carrascosa / Abstract: The double-stranded DNA bacteriophages are good model systems to understand basic biological processes such as the macromolecular interactions that take place during the virus assembly and ...The double-stranded DNA bacteriophages are good model systems to understand basic biological processes such as the macromolecular interactions that take place during the virus assembly and maturation, or the behavior of molecular motors that function during the DNA packaging process. Using cryoelectron microscopy and single-particle methodology, we have determined the structures of two phage T7 assemblies produced during its morphogenetic process, the DNA-free prohead and the mature virion. The first structure reveals a complex assembly in the interior of the capsid, which involves the scaffolding, and the core complex, which plays an important role in DNA packaging and is located in one of the phage vertices. The reconstruction of the mature virion reveals important changes in the shell, now much larger and thinner, the disappearance of the scaffolding structure, and important rearrangements of the core complex, which now protrudes the shell and interacts with the tail. Some of these changes must originate by the pressure exerted by the DNA in the interior of the head.
History
Deposition
Sep 22, 2005
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Header (metadata) release
Sep 22, 2005
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Map release
Sep 22, 2006
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Update
Oct 17, 2012
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Current status
Oct 17, 2012
Processing site: PDBe / Status: Released
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Structure visualization
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Surface view with section colored by density value
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