Biotechnology and Biological Sciences Research Council (BBSRC)
BB/L023091/1
United Kingdom
Citation
Journal: Proc Natl Acad Sci U S A / Year: 2020 Title: An ATP-dependent partner switch links flagellar C-ring assembly with gene expression. Authors: Vitan Blagotinsek / Meike Schwan / Wieland Steinchen / Devid Mrusek / John C Hook / Florian Rossmann / Sven A Freibert / Hanna Kratzat / Guillaume Murat / Dieter Kressler / Roland Beckmann / ...Authors: Vitan Blagotinsek / Meike Schwan / Wieland Steinchen / Devid Mrusek / John C Hook / Florian Rossmann / Sven A Freibert / Hanna Kratzat / Guillaume Murat / Dieter Kressler / Roland Beckmann / Morgan Beeby / Kai M Thormann / Gert Bange / Abstract: Bacterial flagella differ in their number and spatial arrangement. In many species, the MinD-type ATPase FlhG (also YlxH/FleN) is central to the numerical control of bacterial flagella, and its ...Bacterial flagella differ in their number and spatial arrangement. In many species, the MinD-type ATPase FlhG (also YlxH/FleN) is central to the numerical control of bacterial flagella, and its deletion in polarly flagellated bacteria typically leads to hyperflagellation. The molecular mechanism underlying this numerical control, however, remains enigmatic. Using the model species , we show that FlhG links assembly of the flagellar C ring with the action of the master transcriptional regulator FlrA (named FleQ in other species). While FlrA and the flagellar C-ring protein FliM have an overlapping binding site on FlhG, their binding depends on the ATP-dependent dimerization state of FlhG. FliM interacts with FlhG independent of nucleotide binding, while FlrA exclusively interacts with the ATP-dependent FlhG dimer and stimulates FlhG ATPase activity. Our in vivo analysis of FlhG partner switching between FliM and FlrA reveals its mechanism in the numerical restriction of flagella, in which the transcriptional activity of FlrA is down-regulated through a negative feedback loop. Our study demonstrates another level of regulatory complexity underlying the spationumerical regulation of flagellar biogenesis and implies that flagellar assembly transcriptionally regulates the production of more initial building blocks.
History
Deposition
May 21, 2020
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Header (metadata) release
Oct 21, 2020
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Map release
Oct 21, 2020
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Update
Oct 21, 2020
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Current status
Oct 21, 2020
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Model: UltrAuFoil / Material: GOLD / Mesh: 200 / Support film - Material: GOLD / Support film - topology: HOLEY / Support film - Film thickness: 50.0 nm / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.02 kPa
Vitrification
Cryogen name: ETHANE-PROPANE / Chamber humidity: 100 % / Chamber temperature: 298 K / Instrument: FEI VITROBOT MARK IV Details: blot time 5 s, blot force 3, wait time 60s, drain time 0.5s,.
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Electron microscopy
Microscope
FEI TECNAI F20
Electron beam
Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
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