+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-1048 | |||||||||
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タイトル | Three-dimensional structure of bacteriophage T4 baseplate. | |||||||||
マップデータ | map contains the baseplate and the proximal part of the tail tube | |||||||||
試料 |
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機能・相同性 | 機能・相同性情報 symbiont entry into host cell via disruption of host cell wall peptidoglycan / virus tail, baseplate / virus tail, fiber / viral tail assembly / symbiont entry into host cell via disruption of host cell envelope / symbiont entry into host / virus tail / viral release from host cell / peptidoglycan catabolic process / cell wall macromolecule catabolic process ...symbiont entry into host cell via disruption of host cell wall peptidoglycan / virus tail, baseplate / virus tail, fiber / viral tail assembly / symbiont entry into host cell via disruption of host cell envelope / symbiont entry into host / virus tail / viral release from host cell / peptidoglycan catabolic process / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / killing of cells of another organism / entry receptor-mediated virion attachment to host cell / defense response to bacterium / symbiont entry into host cell / identical protein binding / metal ion binding 類似検索 - 分子機能 | |||||||||
生物種 | Enterobacteria phage T4 (ファージ) | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 12.0 Å | |||||||||
データ登録者 | Kostyuchenko VA / Leiman PG / Chipman PR / Kanamaru S / vanRaaij MJ / Arisaka F / Mesyanzhinov VV / Rossmann MG | |||||||||
引用 | ジャーナル: Nat Struct Biol / 年: 2003 タイトル: Three-dimensional structure of bacteriophage T4 baseplate. 著者: Victor A Kostyuchenko / Petr G Leiman / Paul R Chipman / Shuji Kanamaru / Mark J van Raaij / Fumio Arisaka / Vadim V Mesyanzhinov / Michael G Rossmann / 要旨: The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three- ...The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three-dimensional structure of the baseplate-tail tube complex determined to a resolution of 12 A by cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the center of the dome is a needle-like structure that was previously identified as a cell puncturing device. We have identified the locations of six proteins with known atomic structures, and established the position and shape of several other baseplate proteins. The baseplate structure suggests a mechanism of baseplate triggering and structural transition during the initial stages of T4 infection. | |||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_1048.map.gz | 3.5 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-1048-v30.xml emd-1048.xml | 14 KB 14 KB | 表示 表示 | EMDBヘッダ |
画像 | 1048.gif | 53.1 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-1048 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-1048 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_1048_validation.pdf.gz | 350 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_1048_full_validation.pdf.gz | 349.6 KB | 表示 | |
XML形式データ | emd_1048_validation.xml.gz | 6 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-1048 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-1048 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_1048.map.gz / 形式: CCP4 / 大きさ: 28.1 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | map contains the baseplate and the proximal part of the tail tube | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 2.97872 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
-全体 : Bacteriophage T4 baseplate-tail tube assembly
全体 | 名称: Bacteriophage T4 baseplate-tail tube assembly |
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要素 |
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-超分子 #1000: Bacteriophage T4 baseplate-tail tube assembly
超分子 | 名称: Bacteriophage T4 baseplate-tail tube assembly / タイプ: sample / ID: 1000 / 集合状態: the complex has sixfold symmetry / Number unique components: 1 |
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-分子 #1: baseplate-tail tube complex
分子 | 名称: baseplate-tail tube complex / タイプ: protein_or_peptide / ID: 1 / 組換発現: Yes |
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由来(天然) | 生物種: Enterobacteria phage T4 (ファージ) / 株: T4 18-,23- / 別称: bacteriophage T4 / Organelle: baseplate and tail tube |
組換発現 | 生物種: Escherichia coli BE |