+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-10321 | |||||||||||||||
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タイトル | Mus musculus brain neocortex ribosome 60S bound to Ebp1 | |||||||||||||||
マップデータ | ex vivo derived mus musculus P0 brain neocortex ribosome containing eEF2 and EBP1 | |||||||||||||||
試料 |
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機能・相同性 | 機能・相同性情報 5.8S rRNA binding / Protein hydroxylation / translation at postsynapse / Formation of a pool of free 40S subunits / SRP-dependent cotranslational protein targeting to membrane / Major pathway of rRNA processing in the nucleolus and cytosol / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / L13a-mediated translational silencing of Ceruloplasmin expression / GTP hydrolysis and joining of the 60S ribosomal subunit ...5.8S rRNA binding / Protein hydroxylation / translation at postsynapse / Formation of a pool of free 40S subunits / SRP-dependent cotranslational protein targeting to membrane / Major pathway of rRNA processing in the nucleolus and cytosol / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / L13a-mediated translational silencing of Ceruloplasmin expression / GTP hydrolysis and joining of the 60S ribosomal subunit / selenocysteine insertion sequence binding / aminoacyl-tRNA synthetase multienzyme complex / eukaryotic 80S initiation complex / negative regulation of protein neddylation / translation at presynapse / axial mesoderm development / embryonic brain development / negative regulation of formation of translation preinitiation complex / 90S preribosome assembly / TORC2 complex binding / GAIT complex / peroxisome proliferator activated receptor binding / middle ear morphogenesis / A band / alpha-beta T cell differentiation / regulation of G1 to G0 transition / exit from mitosis / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / optic nerve development / positive regulation of axonogenesis / response to aldosterone / retinal ganglion cell axon guidance / homeostatic process / G1 to G0 transition / cell-substrate adhesion / macrophage chemotaxis / lung morphogenesis / growth factor binding / positive regulation of signal transduction by p53 class mediator / blastocyst development / ubiquitin ligase inhibitor activity / protein localization to nucleus / protein targeting / positive regulation of G1/S transition of mitotic cell cycle / positive regulation of axon extension / protein-RNA complex assembly / cellular response to interleukin-4 / cellular response to actinomycin D / negative regulation of ubiquitin-dependent protein catabolic process / ribonucleoprotein complex binding / translation regulator activity / rough endoplasmic reticulum / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / maturation of LSU-rRNA / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / cytosolic ribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Neutrophil degranulation / ossification / cellular response to dexamethasone stimulus / ribosomal large subunit biogenesis / skeletal system development / positive regulation of translation / liver regeneration / positive regulation of cell differentiation / sensory perception of sound / bone development / multicellular organism growth / cellular response to gamma radiation / cellular response to type II interferon / mRNA 5'-UTR binding / transcription coactivator binding / cytoplasmic ribonucleoprotein granule / transcription corepressor activity / rRNA processing / antimicrobial humoral immune response mediated by antimicrobial peptide / large ribosomal subunit / regulation of translation / heparin binding / presynapse / retina development in camera-type eye / 5S rRNA binding / large ribosomal subunit rRNA binding / ribosomal large subunit assembly / cell body / fibroblast proliferation / cytoplasmic translation / cytosolic large ribosomal subunit / defense response to Gram-negative bacterium / response to ethanol / postsynapse / killing of cells of another organism / response to lipopolysaccharide / nucleic acid binding / tRNA binding / negative regulation of translation / postsynaptic density 類似検索 - 分子機能 | |||||||||||||||
生物種 | Mus musculus (ハツカネズミ) / Mouse (ネズミ) | |||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.1 Å | |||||||||||||||
データ登録者 | Kraushar ML / Sprink T | |||||||||||||||
資金援助 | ドイツ, 4件
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引用 | ジャーナル: Mol Cell / 年: 2021 タイトル: Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit. 著者: Matthew L Kraushar / Ferdinand Krupp / Dermot Harnett / Paul Turko / Mateusz C Ambrozkiewicz / Thiemo Sprink / Koshi Imami / Manuel Günnigmann / Ulrike Zinnall / Carlos H Vieira-Vieira / ...著者: Matthew L Kraushar / Ferdinand Krupp / Dermot Harnett / Paul Turko / Mateusz C Ambrozkiewicz / Thiemo Sprink / Koshi Imami / Manuel Günnigmann / Ulrike Zinnall / Carlos H Vieira-Vieira / Theres Schaub / Agnieszka Münster-Wandowski / Jörg Bürger / Ekaterina Borisova / Hiroshi Yamamoto / Mladen-Roko Rasin / Uwe Ohler / Dieter Beule / Thorsten Mielke / Victor Tarabykin / Markus Landthaler / Günter Kramer / Imre Vida / Matthias Selbach / Christian M T Spahn / 要旨: Protein synthesis must be finely tuned in the developing nervous system as the final essential step of gene expression. This study investigates the architecture of ribosomes from the neocortex during ...Protein synthesis must be finely tuned in the developing nervous system as the final essential step of gene expression. This study investigates the architecture of ribosomes from the neocortex during neurogenesis, revealing Ebp1 as a high-occupancy 60S peptide tunnel exit (TE) factor during protein synthesis at near-atomic resolution by cryoelectron microscopy (cryo-EM). Ribosome profiling demonstrated Ebp1-60S binding is highest during start codon initiation and N-terminal peptide elongation, regulating ribosome occupancy of these codons. Membrane-targeting domains emerging from the 60S tunnel, which recruit SRP/Sec61 to the shared binding site, displace Ebp1. Ebp1 is particularly abundant in the early-born neural stem cell (NSC) lineage and regulates neuronal morphology. Ebp1 especially impacts the synthesis of membrane-targeted cell adhesion molecules (CAMs), measured by pulsed stable isotope labeling by amino acids in cell culture (pSILAC)/bioorthogonal noncanonical amino acid tagging (BONCAT) mass spectrometry (MS). Therefore, Ebp1 is a central component of protein synthesis, and the ribosome TE is a focal point of gene expression control in the molecular specification of neuronal morphology during development. | |||||||||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_10321.map.gz | 33.7 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-10321-v30.xml emd-10321.xml | 60 KB 60 KB | 表示 表示 | EMDBヘッダ |
画像 | emd_10321.png | 269.1 KB | ||
その他 | emd_10321_additional_1.map.gz | 21.4 MB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-10321 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-10321 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_10321_validation.pdf.gz | 269.7 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_10321_full_validation.pdf.gz | 268.8 KB | 表示 | |
XML形式データ | emd_10321_validation.xml.gz | 7 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-10321 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-10321 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_10321.map.gz / 形式: CCP4 / 大きさ: 178 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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注釈 | ex vivo derived mus musculus P0 brain neocortex ribosome containing eEF2 and EBP1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.3267 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-添付マップデータ: emd 10321 additional 1.map
ファイル | emd_10321_additional_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-試料の構成要素
+全体 : Mus musculus postnatal day 0 brain neocortex 80S ribosome bound t...
+超分子 #1: Mus musculus postnatal day 0 brain neocortex 80S ribosome bound t...
+分子 #1: 60S ribosomal protein L8
+分子 #2: 60S ribosomal protein L3
+分子 #3: 60S ribosomal protein L4
+分子 #4: 60S ribosomal protein L5
+分子 #5: 60S ribosomal protein L6
+分子 #6: 60S ribosomal protein L7
+分子 #7: 60S ribosomal protein L7a
+分子 #8: 60S ribosomal protein L9
+分子 #9: 60S ribosomal protein L10
+分子 #10: 60S ribosomal protein L11
+分子 #11: 60S ribosomal protein L13
+分子 #12: 60S ribosomal protein L14
+分子 #13: 60S ribosomal protein L15
+分子 #14: 60S ribosomal protein L13a
+分子 #15: 60S ribosomal protein L17
+分子 #16: 60S ribosomal protein L18
+分子 #17: 60S ribosomal protein L18a
+分子 #18: 60S ribosomal protein L21
+分子 #19: 60S ribosomal protein L22
+分子 #20: 60S ribosomal protein L23
+分子 #21: 60S ribosomal protein L24
+分子 #22: 60S ribosomal protein L23a
+分子 #23: Ribosomal protein L26
+分子 #24: 60S ribosomal protein L27
+分子 #25: 60S ribosomal protein L27a
+分子 #26: 60S ribosomal protein L29
+分子 #27: 60S ribosomal protein L30
+分子 #28: 60S ribosomal protein L31
+分子 #29: 60S ribosomal protein L32
+分子 #30: 60S ribosomal protein L35a
+分子 #31: 60S ribosomal protein L34
+分子 #32: 60S ribosomal protein L35
+分子 #33: 60S ribosomal protein L36
+分子 #34: 60S ribosomal protein L37
+分子 #35: 60S ribosomal protein L38
+分子 #36: Ribosomal protein L39
+分子 #37: 60S ribosomal protein L40
+分子 #38: 60S ribosomal protein L41
+分子 #39: Ribosomal protein L36A
+分子 #40: Ribosomal protein L37a
+分子 #41: 60S ribosomal protein L19
+分子 #42: 60S ribosomal protein L28
+分子 #46: Proliferation-associated protein 2G4
+分子 #43: 28S ribosomal RNA
+分子 #44: 5.8S ribosomal RNA
+分子 #45: 5S ribosomal RNA
+分子 #47: MAGNESIUM ION
+分子 #48: ZINC ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.4 構成要素:
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グリッド | モデル: Quantifoil R3/3 / 材質: COPPER / メッシュ: 100 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: HOLEY / 支持フィルム - Film thickness: 2.0 nm / 前処理 - タイプ: GLOW DISCHARGE | ||||||||||||||||||||||||
凍結 | 凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK II |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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特殊光学系 | エネルギーフィルター - 名称: GIF Quantum LS |
撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 実像数: 5379 / 平均露光時間: 20.0 sec. / 平均電子線量: 31.78 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス(公称値): 2.5 µm / 最小 デフォーカス(公称値): 0.5 µm / 倍率(公称値): 31000 |
試料ステージ | 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER ホルダー冷却材: NITROGEN |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |