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- EMDB-10284: Pseudomonas aeruginosa 70s ribosome from an aminoglycoside resist... -

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Basic information

Entry
Database: EMDB / ID: EMD-10284
TitlePseudomonas aeruginosa 70s ribosome from an aminoglycoside resistant clinical isolate
Map data
Sample
  • Complex: Pseudomonas aeruginosa 70s ribosome from a clinical isolate
    • RNA: x 3 types
    • Protein or peptide: x 50 types
Function / homology
Function and homology information


large ribosomal subunit rRNA binding / large ribosomal subunit / small ribosomal subunit / 5S rRNA binding / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation ...large ribosomal subunit rRNA binding / large ribosomal subunit / small ribosomal subunit / 5S rRNA binding / transferase activity / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / metal ion binding / cytoplasm
Similarity search - Function
Type-1 KH domain profile. / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L11, bacterial-type / Ribosomal protein L31 type A ...Type-1 KH domain profile. / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L11, bacterial-type / Ribosomal protein L31 type A / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein L31 signature. / Ribosomal protein S21 / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L11, conserved site / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L11 signature. / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L11, N-terminal / Ribosomal protein L17 signature. / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11, RNA binding domain / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L32p, bacterial type / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein S6, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S6 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L14P, bacterial-type / Ribosomal protein S4, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / :
Similarity search - Domain/homology
Small ribosomal subunit protein uS9 / Small ribosomal subunit protein bS6 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL35 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS15 ...Small ribosomal subunit protein uS9 / Small ribosomal subunit protein bS6 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL35 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL25 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein bL17 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein bL21 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL9 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein bL34 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL11 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS11
Similarity search - Component
Biological speciesPseudomonas aeruginos (bacteria) / Pseudomonas aeruginosa (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.89 Å
AuthorsHalfon Y / Jimenez-Fernande A / La Ros R / Espinos R / Krogh Johansen H / Matzov D / Eyal Z / Bashan A / Zimmerman E / Belousoff M ...Halfon Y / Jimenez-Fernande A / La Ros R / Espinos R / Krogh Johansen H / Matzov D / Eyal Z / Bashan A / Zimmerman E / Belousoff M / Molin S / Yonath A
Funding support Denmark, 4 items
OrganizationGrant numberCountry
Danish Council for Independent ResearchDFF-4181-00115 Denmark
European Research Council322581
Novo Nordisk Foundation Denmark
European UnioniNEXT, project number 653706, funded by the Horizon 2020 programme of the European Union
CitationJournal: Proc Natl Acad Sci U S A / Year: 2019
Title: Structure of ribosomes from an aminoglycoside-resistant clinical isolate.
Authors: Yehuda Halfon / Alicia Jimenez-Fernandez / Ruggero La Rosa / Rocio Espinosa Portero / Helle Krogh Johansen / Donna Matzov / Zohar Eyal / Anat Bashan / Ella Zimmerman / Matthew Belousoff / ...Authors: Yehuda Halfon / Alicia Jimenez-Fernandez / Ruggero La Rosa / Rocio Espinosa Portero / Helle Krogh Johansen / Donna Matzov / Zohar Eyal / Anat Bashan / Ella Zimmerman / Matthew Belousoff / Søren Molin / Ada Yonath /
Abstract: Resistance to antibiotics has become a major threat to modern medicine. The ribosome plays a fundamental role in cell vitality by the translation of the genetic code into proteins; hence, it is a ...Resistance to antibiotics has become a major threat to modern medicine. The ribosome plays a fundamental role in cell vitality by the translation of the genetic code into proteins; hence, it is a major target for clinically useful antibiotics. We report here the cryo-electron microscopy structures of the ribosome of a pathogenic aminoglycoside (AG)-resistant strain, as well as of a nonresistance strain isolated from a cystic fibrosis patient. The structural studies disclosed defective ribosome complex formation due to a conformational change of rRNA helix H69, an essential intersubunit bridge, and a secondary binding site of the AGs. In addition, a stable conformation of nucleotides A1486 and A1487, pointing into helix h44, is created compared to a non-AG-bound ribosome. We suggest that altering the conformations of ribosomal protein uL6 and rRNA helix H69, which interact with initiation-factor IF2, interferes with proper protein synthesis initiation.
History
DepositionSep 1, 2019-
Header (metadata) releaseOct 16, 2019-
Map releaseOct 23, 2019-
UpdateDec 2, 2020-
Current statusDec 2, 2020Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0491
  • Imaged by UCSF Chimera
  • Download
  • Surface view colored by height
  • Surface level: 0.0491
  • Imaged by UCSF Chimera
  • Download
  • Surface view with fitted model
  • Atomic models: PDB-6spf
  • Surface level: 0.0491
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_10284.png

Downloads & links

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Map

FileDownload / File: emd_10284.map.gz / Format: CCP4 / Size: 244.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.1 Å
Density
Contour LevelBy AUTHOR: 0.0491 / Movie #1: 0.0491
Minimum - Maximum-0.478421 - 0.7881263
Average (Standard dev.)0.0007639024 (±0.017213747)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions400400400
Spacing400400400
CellA=B=C: 440.0 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.11.11.1
M x/y/z400400400
origin x/y/z0.0000.0000.000
length x/y/z440.000440.000440.000
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS400400400
D min/max/mean-0.4780.7880.001

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Supplemental data

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Additional map: Unsharp map

Fileemd_10284_additional.map
AnnotationUnsharp map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Pseudomonas aeruginosa 70s ribosome from a clinical isolate

EntireName: Pseudomonas aeruginosa 70s ribosome from a clinical isolate
Components
  • Complex: Pseudomonas aeruginosa 70s ribosome from a clinical isolate
    • RNA: Pseudomonas aeruginosa strain PAO1 23S ribosomal RNA
    • RNA: 5S rRNA5S ribosomal RNA
    • Protein or peptide: 50S ribosomal protein L2
    • Protein or peptide: 50S ribosomal protein L3
    • Protein or peptide: 50S ribosomal protein L4
    • Protein or peptide: 50S ribosomal protein L5
    • Protein or peptide: 50S ribosomal protein L6
    • Protein or peptide: 50S ribosomal protein L9
    • Protein or peptide: 50S ribosomal protein L11
    • Protein or peptide: 50S ribosomal protein L13
    • Protein or peptide: 50S ribosomal protein L14
    • Protein or peptide: 50S ribosomal protein L15
    • Protein or peptide: 50S ribosomal protein L16
    • Protein or peptide: 50S ribosomal protein L17
    • Protein or peptide: 50S ribosomal protein L18
    • Protein or peptide: 50S ribosomal protein L19
    • Protein or peptide: 50S ribosomal protein L20
    • Protein or peptide: 50S ribosomal protein L21
    • Protein or peptide: 50S ribosomal protein L22
    • Protein or peptide: 50S ribosomal protein L23
    • Protein or peptide: 50S ribosomal protein L24
    • Protein or peptide: 50S ribosomal protein L25
    • Protein or peptide: 50S ribosomal protein L27
    • Protein or peptide: 50S ribosomal protein L28
    • Protein or peptide: Ribosomal protein uL29
    • Protein or peptide: 50S ribosomal protein L30
    • Protein or peptide: 50S ribosomal protein L31
    • Protein or peptide: 50S ribosomal protein L32
    • Protein or peptide: 50S ribosomal protein L33
    • Protein or peptide: 50S ribosomal protein L34
    • Protein or peptide: 50S ribosomal protein L35
    • Protein or peptide: 50S ribosomal protein L36
    • RNA: 16S rRNA
    • Protein or peptide: 30S ribosomal protein S2
    • Protein or peptide: 30S ribosomal protein S3
    • Protein or peptide: 30S ribosomal protein S4
    • Protein or peptide: 30S ribosomal protein S5
    • Protein or peptide: 30S ribosomal protein S6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: 30S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S9
    • Protein or peptide: 30S ribosomal protein S10
    • Protein or peptide: 30S ribosomal protein S11
    • Protein or peptide: 30S ribosomal protein S12
    • Protein or peptide: 30S ribosomal protein S13
    • Protein or peptide: 30S ribosomal protein S14
    • Protein or peptide: 30S ribosomal protein S15
    • Protein or peptide: 30S ribosomal protein S16
    • Protein or peptide: 30S ribosomal protein S17
    • Protein or peptide: 30S ribosomal protein S18
    • Protein or peptide: 30S ribosomal protein S19
    • Protein or peptide: 30S ribosomal protein S20
    • Protein or peptide: 30S ribosomal protein S21

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Supramolecule #1: Pseudomonas aeruginosa 70s ribosome from a clinical isolate

SupramoleculeName: Pseudomonas aeruginosa 70s ribosome from a clinical isolate
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#53
Source (natural)Organism: Pseudomonas aeruginos (bacteria)

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Macromolecule #1: Pseudomonas aeruginosa strain PAO1 23S ribosomal RNA

MacromoleculeName: Pseudomonas aeruginosa strain PAO1 23S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 935.990438 KDa
SequenceString: GUCAAGUGAA GAAGCGCAUA CGGUGGAUGC CUUGGCAGUC AGAGGCGAUG AAAGACGUGG UAGCCUGCGA AAAGCUUCGG GGAGUCGGC AAACAGACUU UGAUCCGGAG AUCUCUGAAU GGGGGAACCC ACCUAGGAUA ACCUAGGUAU CUUGUACUGA A UCCAUAGG ...String:
GUCAAGUGAA GAAGCGCAUA CGGUGGAUGC CUUGGCAGUC AGAGGCGAUG AAAGACGUGG UAGCCUGCGA AAAGCUUCGG GGAGUCGGC AAACAGACUU UGAUCCGGAG AUCUCUGAAU GGGGGAACCC ACCUAGGAUA ACCUAGGUAU CUUGUACUGA A UCCAUAGG UGCAAGAGGC GAACCAGGGG AACUGAAACA UCUAAGUACC CUGAGGAAAA GAAAUCAACC GAGAUUCCCU UA GUAGUGG CGAGCGAACG GGGAUUAGCC CUUAAGCUUC AUUGAUUUUA GCGGAACGCU CUGGAAAGUG CGGCCAUAGU GGG UGAUAG CCCCGUACGC GAAAGGAUCU UUGAAGUGAA AUCAAGUAGG ACGGAGCACG AGAAACUUUG UCUGAACAUG GGGG GACCA UCCUCCAAGG CUAAAUACUA CUGACUGACC GAUAGUGAAC CAGUACCGUG AGGGAAAGGC GAAAAGAACC CCGGA GAGG GGAGUGAAAU AGAACCUGAA ACCGUAUGCG UACAAGCAGU GGGAGCCUAC UUGUUAGGUG ACUGCGUACC UUUUGU AUA AUGGGUCAGC GACUUAUAUU CAGUGGCAAG CUUAACCGUA UAGGGUAGGC GUAGCGAAAG CGAGUCUUAA UAGGGCG UU UAGUCGCUGG GUAUAGACCC GAAACCGGGC GAUCUAUCCA UGAGCAGGUU GAAGGUUAGG UAACACUGAC UGGAGGAC C GAACCCACUC CCGUUGAAAA GGUAGGGGAU GACUUGUGGA UCGGAGUGAA AGGCUAAUCA AGCUCGGAGA UAGCUGGUU CUCCUCGAAA GCUAUUUAGG UAGCGCCUCA UGUAUCACUC UGGGGGGUAG AGCACUGUUU CGGCUAGGGG GUCAUCCCGA CUUACCAAA CCGAUGCAAA CUCCGAAUAC CCAGAAGUGC CGAGCAUGGG AGACACACGG CGGGUGCUAA CGUCCGUCGU G AAAAGGGA AACAACCCAG ACCGCCAGCU AAGGUCCCAA AGUUGUGGUU AAGUGGUAAA CGAUGUGGGA AGGCUUAGAC AG CUAGGAG GUUGGCUUAG AAGCAGCCAC CCUUUAAAGA AAGCGUAAUA GCUCACUAGU CGAGUCGGCC UGCGCGGAAG AUG UAACGG GGCUCAAACC ACACACCGAA GCUGCGGGUG UCACGUAAGU GACGCGGUAG AGGAGCGUUC UGUAAGCCUG UGAA GGUGA GUUGAGAAGC UUGCUGGAGG UAUCAGAAGU GCGAAUGCUG ACAUGAGUAA CGACAAUGGG UGUGAAAAAC ACCCA CGCC GAAAGACCAA GGGUUCCUGC GCAACGUUAA UCGACGCAGG GUUAGUCGGU UCCUAAGGCG AGGCUGAAAA GCGUAG UCG AUGGGAAACA GGUUAAUAUU CCUGUACUUC UGGUUACUGC GAUGGAGGGA CGGAGAAGGC UAGGCCAGCU UGGCGUU GG UUGUCCAAGU UUAAGGUGGU AGGCUGAAAU CUUAGGUAAA UCCGGGGUUU CAAGGCCGAG AGCUGAUGAC GAGUCGUC U UUUAGAUGAC GAAGUGGUUG AUGCCAUGCU UCCAAGAAAA GCUUCUAAGC UUCAGGUAAC CAGGAACCGU ACCCCAAAC CGACACAGGU GGUCGGGUAG AGAAUACCAA GGCGCUUGAG AGAACUCGGG UGAAGGAACU AGGCAAAAUG GCACCGUAAC UUCGGGAGA AGGUGCGCCG GCUAGGGUGA AGGAUUUACU CCGUAAGCUC UGGCUGGUCG AAGAUACCAG GCCGCUGCGA C UGUUUAUU AAAAACACAG CACUCUGCAA ACACGAAAGU GGACGUAUAG GGUGUGACGC CUGCCCGGUG CCGGAAGGUU AA UUGAUGG GGUUAGCGCA AGCGAAGCUC UUGAUCGAAG CCCCGGUAAA CGGCGGCCGU AACUAUAACG GUCCUAAGGU AGC GAAAUU CCUUGUCGGG UAAGUUCCGA CCUGCACGAA UGGCGUAACG AUGGCGGCGC UGUCUCCACC CGAGACUCAG UGAA AUUGA AAUCGCUGUG AAGAUGCAGU GUAUCCGCGG CUAGACGGAA AGACCCCGUG AACCUUUACU GUAGCUUUGC ACUGG ACUU UGAGCCUGCU UGUGUAGGAU AGGUGGGAGG CUUUGAAGCG UGGACGCCAG UUCGCGUGGA GCCAUCCUUG AAAUAC CAC CCUGGCAUGC UUGAGGUUCU AACUCUGGUC CGUAAUCCGG AUCGAGGACA GUGUAUGGUG GGCAGUUUGA CUGGGGC GG UCUCCUCCUA AAGAGUAACG GAGGAGUACG AAGGUGCGCU CAGACCGGUC GGAAAUCGGU CGCAGAGUAU AAAGGCAA A AGCGCGCUUG ACUGCGAGAC AGACACGUCG AGCAGGUACG AAAGUAGGUC UUAGUGAUCC GGUGGUUCUG UAUGGAAGG GCCAUCGCUC AACGGAUAAA AGGUACUCCG GGGAUAACAG GCUGAUACCG CCCAAGAGUU CAUAUCGACG GCGGUGUUUG GCACCUCGA UGUCGGCUCA UCACAUCCUG GGGCUGAAGC CGGUCCCAAG GGUAUGGCUG UUCGCCAUUU AAAGUGGUAC G CGAGCUGG GUUUAGAACG UCGUGAGACA GUUCGGUCCC UAUCUGCCGU GGACGUUUGA GAUUUGAGAG GGGCUGCUCC UA GUACGAG AGGACCGGAG UGGACGAACC UCUGGUGUUC CGGUUGUCAC GCCAGUGGCA UUGCCGGGUA GCUAUGUUCG GAA AAGAUA ACCGCUGAAA GCAUCUAAGC GGGAAACUUG CCUCAAGAUG AGAUCUCACU GGGAACUUGA UUCCCCUGAA GGGC CGUCG AAGACUACGA CGUUGAUAGG CUGGGUGUGU AAGCGUUGUG AGGCGUUGAG CUAACCAGUA CUAAUGGCCC GUGAG GCUU GACC

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Macromolecule #2: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 37.679391 KDa
SequenceString:
GCUUGACGAU CAUAGAGCGU UGGAACCACC UGAUCCCUUC CCGAACUCAG AAGUGAAACG ACGCAUCGCC GAUGGUAGUG UGGGGUCUC CCCAUGUGAG AGUAGGUCAU CGUCAAGC

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Macromolecule #33: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 33 / Number of copies: 1
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 493.068969 KDa
SequenceString: GAAGAGUUUG AUCAUGGCUC AGAUUGAACG CUGGCGGCAG GCCUAACACA UGCAAGUCGA GCGGAUGAAG GGAGCUUGCU CCUGGAUUC AGCGGCAGAC GGGUGAGUAA UGCCUAGGAA UCUGCCUGGU AGUGGGGGAU AACGUCCGGA AACGGGCGCU A AUACCGCA ...String:
GAAGAGUUUG AUCAUGGCUC AGAUUGAACG CUGGCGGCAG GCCUAACACA UGCAAGUCGA GCGGAUGAAG GGAGCUUGCU CCUGGAUUC AGCGGCAGAC GGGUGAGUAA UGCCUAGGAA UCUGCCUGGU AGUGGGGGAU AACGUCCGGA AACGGGCGCU A AUACCGCA UACGUCCUGA GGGAGAAAGU GGGGGAUCUU CGGACCUCAC GCUAUCAGAU GAGCCUAGGU CGGAUUAGCU AG UUGGUGG GGUAAAGGCC UACCAAGGCG ACGAUCCGUA ACUGGUCUGA GAGGAUGAUC AGUCACACUG GAACUGAGAC ACG GUCCAG ACUCCUACGG GAGGCAGCAG UGGGGAAUAU UGGACAAUGG GCGAAAGCCU GAUCCAGCCA UGCCGCGUGU GUGA AGAAG GUCUUCGGAU UGUAAAGCAC UUUAAGUUGG GAGGAAGGGC AGUAAGUUAA UACCUUGCUG UUUUGACGUU ACCAA CAGA AUAAGCACCG GCUAACUUCG UGCCAGCAGC CGCGGUAAUA CGAAGGGUGC AAGCGUUAAU CGGAAUUACU GGGCGU AAA GCGCGCGUAG GUGGUUCAGC AAGUUGGAUG UGAAAUCCCC GGGCUCAACC UGGGAACUGC AUCCAAAACU ACUGAGC UA GAGUACGGUA GAGGGUGGUG GAAUUUCCUG UGUAGCGGUG AAAUGCGUAG AUAUAGGAAG GAACACCAGU GGCGAAGG C GACCACCUGG ACUGAUACUG ACACUGAGGU GCGAAAGCGU GGGGAGCAAA CAGGAUUAGA UACCCUGGUA GUCCACGCC GUAAACGAUG UCGACUAGCC GUUGGGAUCC UUGAGAUCUU AGUGGCGCAG CUAACGCGAU AAGUCGACCG CCUGGGGAGU ACGGCCGCA AGGUUAAAAC UCAAAUGAAU UGACGGGGGC CCGCACAAGC GGUGGAGCAU GUGGUUUAAU UCGAAGCAAC G CGAAGAAC CUUACCUGGC CUUGACAUGC UGAGAACUUU CCAGAGAUGG AUUGGUGCCU UCGGGAACUC AGACACAGGU GC UGCAUGG CUGUCGUCAG CUCGUGUCGU GAGAUGUUGG GUUAAGUCCC GUAACGAGCG CAACCCUUGU CCUUAGUUAC CAG CACCUC GGGUGGGCAC UCUAAGGAGA CUGCCGGUGA CAAACCGGAG GAAGGUGGGG AUGACGUCAA GUCAUCAUGG CCCU UACGG CCAGGGCUAC ACACGUGCUA CAAUGGUCGG UACAAAGGGU UGCCAAGCCG CGAGGUGGAG CUAAUCCCAU AAAAC CGAU CGUAGUCCGG AUCGCAGUCU GCAACUCGAC UGCGUGAAGU CGGAAUCGCU AGUAAUCGUG AAUCAGAAUG UCACGG UGA AUACGUUCCC GGGCCUUGUA CACACCGCCC GUCACACCAU GGGAGUGGGU UGCUCCAGAA GUAGCUAGUC UAACCGC AA GGGGGACGGU UACCACGGAG UGAUUCAUGA CUGGGGUGAA GUCGUAACAA GGUAGCCGUA GGGGAACCUG CGGCUGGA U

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Macromolecule #3: 50S ribosomal protein L2

MacromoleculeName: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 29.438982 KDa
SequenceString: IVKCKPTSAG RRFVVKVVNQ ELHKGAPYAP LLEKKSKSGG RNNNGRITTR HIGGGHKQHY RLVDFRRNKD GIPAIVERVE YDPNRTAHI ALLKYADGER RYIIAPKGVA AGDQLISGIG APIKAGNSMP LRNIPVGSTV HGIELKPGKG AQIARSAGAS A QLVAREGA ...String:
IVKCKPTSAG RRFVVKVVNQ ELHKGAPYAP LLEKKSKSGG RNNNGRITTR HIGGGHKQHY RLVDFRRNKD GIPAIVERVE YDPNRTAHI ALLKYADGER RYIIAPKGVA AGDQLISGIG APIKAGNSMP LRNIPVGSTV HGIELKPGKG AQIARSAGAS A QLVAREGA YVTLRLRSGE MRKVLAECRA TLGEVSNSEH SLRSLGKAGA TRWRGVRPTV RGVAMNPVDH PHGGGEGRTS AG RHPVSPW GLQTKGKKTR SNKRTDNMIV RRRK

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Macromolecule #4: 50S ribosomal protein L3

MacromoleculeName: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 22.17915 KDa
SequenceString: IGVVGRKCGM TRIFTEEGVS IPVTVIEVEP NRVTQFKTEE TDGYRAVQVT AGERRASRVT KAQAGHFAKA NVAAGRGVWE FRLGEEQYA AGDQITVDLF QAGQMVDVTG ESKGKGFAGT IKRWNFRGQD NTHGNSVSHR VPGSIGQCQT PGRVFKGKKM S GHLGAERV ...String:
IGVVGRKCGM TRIFTEEGVS IPVTVIEVEP NRVTQFKTEE TDGYRAVQVT AGERRASRVT KAQAGHFAKA NVAAGRGVWE FRLGEEQYA AGDQITVDLF QAGQMVDVTG ESKGKGFAGT IKRWNFRGQD NTHGNSVSHR VPGSIGQCQT PGRVFKGKKM S GHLGAERV TVQSLEIVRV DAERNLLLVK GAVPGATGGD VIVRPAAKA

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Macromolecule #5: 50S ribosomal protein L4

MacromoleculeName: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 21.542744 KDa
SequenceString: QLNVNGAQAI EVSERTFGGE FNETLVHQAV VAYMAGGRQG SKAQKTRSEV SGGGKKPWRQ KGTGRARAGT IRSPIWRGGG TTFAAKPRS HEQKLNKKMY RAALRSILAE LVRLDRLVVV ADFAVDAPKT KGLVAKLDTL GLKDVLIVTD GVDENLYLAA R NLAHVDVR ...String:
QLNVNGAQAI EVSERTFGGE FNETLVHQAV VAYMAGGRQG SKAQKTRSEV SGGGKKPWRQ KGTGRARAGT IRSPIWRGGG TTFAAKPRS HEQKLNKKMY RAALRSILAE LVRLDRLVVV ADFAVDAPKT KGLVAKLDTL GLKDVLIVTD GVDENLYLAA R NLAHVDVR DVQGSDPVSL IAYDKVLVTV SAVKKFEELL G

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Macromolecule #6: 50S ribosomal protein L5

MacromoleculeName: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 19.804086 KDa
SequenceString:
RLKELYRKEI APKLKEELQL ANVMEVPRVT KITLNMGLGE AVGDKKIIEN AVADLEKITG QKPVVTYARK SIAGFKIREG WPIGVKVTL RSDRMYEFLD RLLSISLPRV RDFRGLNAKS FDGRGNYSMG VKEQIIFPEI DYDKIDALRG LDITLTTTAR T DDEGRALL RAFKFP

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Macromolecule #7: 50S ribosomal protein L6

MacromoleculeName: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 18.189924 KDa
SequenceString:
SRVAKNPVKL PAGVEIKLAG QQLSIKGAKG ALELKVHPSV EVIQDSGELR FAARNGDQQT RAMAGTTRAL VNNMVVGVSQ GFERKLQLV GVQAKGQVLS LSLGFSHPVD YELPAGIVAE TPSQTDILIK GIDKQLVGQV AAEIRDFRPP EPYKGKGVRY A DEVVRRKE A

+
Macromolecule #8: 50S ribosomal protein L9

MacromoleculeName: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 8.216423 KDa
SequenceString:
EVILLEKVAN LGNLGDKVNI KGGYARNFLL PQGKATVATA ENVAAFEARR AELEKAAAEK KAAAEARAAQ LSELVVTL

+
Macromolecule #9: 50S ribosomal protein L11

MacromoleculeName: 50S ribosomal protein L11 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 14.623716 KDa
SequenceString:
KKIQAYIKLQ VKAGQANPSP PVGPALGQHG VNIMEFCKAF NAKTQGQEPG LPTPVIITVY SDRSFTFETK STPAAVLLKK AAGITSGSA RPNSQKVGTV TRAQLEEIAK TKQADLTAAD LDAAVRTIAG SARSMGLNVE G

+
Macromolecule #10: 50S ribosomal protein L13

MacromoleculeName: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 15.927382 KDa
SequenceString:
KTYTAKPETV QRDWFVVDAA GQTLGRLATE IARRLRGKHK PEYTPHVDTG DYIVVINAEQ VRVTGAKTTD KMYYHHSGFP GGIKSINFE KLIAKAPERV IETAVKGMLP KNPLGRDMYR KLKVYKGASH PHTAQQPQEL KI

+
Macromolecule #11: 50S ribosomal protein L14

MacromoleculeName: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 13.223621 KDa
SequenceString:
MIQTQSMLDV ADNSGARRVM CIKVLGGSHR RYAGIGDIIK VTVKEAIPRG KVKKGQVMTA VVVRTKHGVR RTDGSIIRFD GNAAVLLNN KQEPIGTRIF GPVTRELRTE KFMKIVSLAP E

+
Macromolecule #12: 50S ribosomal protein L15

MacromoleculeName: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 15.204488 KDa
SequenceString:
MQLNDLRSAP GARREKHRPG RGIGSGLGKT GGRGHKGLTS RSGGKVAPGF EGGQQPLHRR LPKFGFVSLK AMDRAEVRTS ELAKVEGDV VSLQTLKDAN LINQHVQRVK VMLSGEVGRA VTLKGIAATK GARAAIEAAG GKFED

+
Macromolecule #13: 50S ribosomal protein L16

MacromoleculeName: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 15.303034 KDa
SequenceString:
MLQPKRTKFR KQMTGHNRGL AHRGSKVSFG EYALKATSRG RLTARQIESA RRALTRHVKR GGKIWIRVFP DKPVTKKPLE VRMGKGKGG VEYWVAQIQP GKVLYEIEGV SEELAREAFA LAAAKLPLAT SFVKRTV

+
Macromolecule #14: 50S ribosomal protein L17

MacromoleculeName: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 13.692035 KDa
SequenceString:
MRHRKSGRHL SRTSAHRKAM FQNMAVSLFE HELIKTTLPK AKELRRVAEP LITLAKEDSV ANRRLAFDRT RSKAAVGKLF NDLGKRYAN RPGGYLRILK CGFRAGDNAP MAYVELVDRP V

+
Macromolecule #15: 50S ribosomal protein L18

MacromoleculeName: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 12.55647 KDa
SequenceString:
SVKKETRLRR ARKARLKMRE LETVRLCVYR SSQHIYAQVI AADGGKVLAS ASTLDKDLRE GATGNIDAAK KVGQLVAERA KAAGVTQVA FDRSGFKYHG RVKALADAAR EGGLEF

+
Macromolecule #16: 50S ribosomal protein L19

MacromoleculeName: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 12.824898 KDa
SequenceString:
TNKIIQQIEA EQMNKEIPAF APGDTVIVQV KVKEGDRQRL QAFEGVVIAK RNRGLNSAFT VRKISNGVGV ERTFQTYSPI VDSLSVKRR GDVRKAKLYY LRALSGKAAR IKEKL

+
Macromolecule #17: 50S ribosomal protein L20

MacromoleculeName: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 13.266776 KDa
SequenceString:
ARVKRGVIAR RRHKKILKLA KGYYGARSRV FRVAKQAVIK AGQYAYRDRR QRKRQFRALW IARINAGARQ NGLSYSRLIA GLKKAAIEI DRKVLADLAV NEKAAFTAIV EKAKASLA

+
Macromolecule #18: 50S ribosomal protein L21

MacromoleculeName: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 11.607544 KDa
SequenceString:
MYAVIVTGGK QHKVTEGEFL KVEKLDVATG EAIDFDRVLL VANGEDVKIG LPVVEGAKVT AEVVSHGRHD KVRIIKFRRR KHHMKRQGH RQWFTEIKIT GIQ

+
Macromolecule #19: 50S ribosomal protein L22

MacromoleculeName: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 11.935996 KDa
SequenceString:
MEVAAKLSGA RISAQKARLV ADQIRGKKVG EALNLLAFSS KKAAEIMKKV LESAVANAEH NEGADVDDLK VSTVFVNEGR SLKRIMPRA KGRADRIVKR SCHITVKVAD K

+
Macromolecule #20: 50S ribosomal protein L23

MacromoleculeName: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 10.510264 KDa
SequenceString:
MNQERVFKVL LGPHISEKAT GLADGKSQFV FKVATDATKL EIKKAVESLF SVKVQRVTTL NVKGKTKRTA RGLGKRNDWK KAYIALQPG QDLDF

+
Macromolecule #21: 50S ribosomal protein L24

MacromoleculeName: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 11.423413 KDa
SequenceString:
MQKIRRDDEV IVIAGKDKGK RGKVLKVLAD DRLVVGGVNL IKRHTKPNPM LGQQGGIVEK EAPLHVSNVA IFNTETSKAD RVGFKVEDG KKIRVFKSTQ KPVQ

+
Macromolecule #22: 50S ribosomal protein L25

MacromoleculeName: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 20.346504 KDa
SequenceString:
FIVNAQVRSD LGKGASRRLR RNAGLVPAVV YGGDKEPQSV TLELREIAKL LENEAAFSHV IALNVGGVKE TVLIKALQRH PAKGFVMHA DFLRVVADHK LTAHVPLHFI NEEVAVGVKQ AGGEISHTIS EVEVSCLPKD LPEFIEVDMA KVELGQIVHL S DLKAPKGV ELVQLAHGND LAVANIHASR

+
Macromolecule #23: 50S ribosomal protein L27

MacromoleculeName: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 8.124437 KDa
SequenceString:
TRNGRDSESK RLGVKLFGGQ AVKAGNILVR LRGTKFHAGY GVGLGKDHTL FAKVDGVVKF ETKGAFGRKY VSIVAA

+
Macromolecule #24: 50S ribosomal protein L28

MacromoleculeName: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 8.956437 KDa
SequenceString:
SRVCQVTGKG PVTGNNISHA HNKTRRRFLP NLQHHRFWVE SEKRFVRLRV SAKGMRIIDK RGIEAVLADL RARGEKF

+
Macromolecule #25: Ribosomal protein uL29

MacromoleculeName: Ribosomal protein uL29 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 6.807757 KDa
SequenceString:
KANELREEKS GPVTGNNISH LLRDQFNLRM QKATGQLGQS HLLSQVKRDI ARVKTVLNQQ

+
Macromolecule #26: 50S ribosomal protein L30

MacromoleculeName: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 6.358448 KDa
SequenceString:
ATVKVTLVKS LNGRLANHKA CVKGLGLRRI NHTVEVQDTP ENRGMINKAY YLLRVEG

+
Macromolecule #27: 50S ribosomal protein L31

MacromoleculeName: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 3.368921 KDa
SequenceString:
KADIHPTYEA IEATCSCGNV IKTRSTLCKP I

+
Macromolecule #28: 50S ribosomal protein L32

MacromoleculeName: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 6.043777 KDa
SequenceString:
AVQQNKKSRS ARDMRRSHDA LESNALSVEK STGEVHLRHH VSPDGFYRGR KVV

+
Macromolecule #29: 50S ribosomal protein L33

MacromoleculeName: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 5.928945 KDa
SequenceString:
RELIRLVSSA GTGHFYTTDK NKRTKPEKIE IKKYDPVVRQ HVIYKEAKIK

+
Macromolecule #30: 50S ribosomal protein L34

MacromoleculeName: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 5.226309 KDa
SequenceString:
MKRTFQPSTL KRARVHGFRA RMATKNGRQV LSRRRAKGRK RLTV

+
Macromolecule #31: 50S ribosomal protein L35

MacromoleculeName: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 7.2568 KDa
SequenceString:
PKMKTKSGAA KRFKKTAGGL KHKHAFKSHI LTKMTTKRKR QLRGTSMLNK SDVARVERSL RLR

+
Macromolecule #32: 50S ribosomal protein L36

MacromoleculeName: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 4.447445 KDa
SequenceString:
MKVRASVKKL CRNCKIIRRD GIVRVICSAE PRHKQRQG

+
Macromolecule #34: 30S ribosomal protein S2

MacromoleculeName: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 25.366418 KDa
SequenceString: QVNMRDMLKA GVHFGHQTRY WNPKMGKFIF GARNKIHIIN LEKTLPMFNE ALTFVERLAA GKNKILFVGT KRSAGKIVRE EAARCGMPY VDHRWLGGML TNYKTIRQSI KRLRDLETQS QDGTFDKLTK KEALMRSRDL EKLERSLGGI KDMGGLPDAL F VIDVDHER ...String:
QVNMRDMLKA GVHFGHQTRY WNPKMGKFIF GARNKIHIIN LEKTLPMFNE ALTFVERLAA GKNKILFVGT KRSAGKIVRE EAARCGMPY VDHRWLGGML TNYKTIRQSI KRLRDLETQS QDGTFDKLTK KEALMRSRDL EKLERSLGGI KDMGGLPDAL F VIDVDHER IAITEANKLG IPVIGVVDTN SSPEGVDYVI PGNDDAIRAV QLYLNSMAEA VIRGKQGA

+
Macromolecule #35: 30S ribosomal protein S3

MacromoleculeName: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 23.180906 KDa
SequenceString: VHPNGIRLGI VKEHTSVWYA DRKNYADYLF ADLKVREYLQ DKLKSASVSR IDIHRPAQTA RITIHTARPG IVIGKKGEDV EKLRQDLTK QMGVPVHINI EEIRKPELDA MLVAQSVAQQ LERRVMFRRA MKRAVQNAMR IGAKGIKIQV SGRLGGAEIA R TEWYREGR ...String:
VHPNGIRLGI VKEHTSVWYA DRKNYADYLF ADLKVREYLQ DKLKSASVSR IDIHRPAQTA RITIHTARPG IVIGKKGEDV EKLRQDLTK QMGVPVHINI EEIRKPELDA MLVAQSVAQQ LERRVMFRRA MKRAVQNAMR IGAKGIKIQV SGRLGGAEIA R TEWYREGR VPLHTLRADI DYATYEAHTT YGVIGVKVWI FKGEV

+
Macromolecule #36: 30S ribosomal protein S4

MacromoleculeName: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 23.116396 KDa
SequenceString: RYIGPKCKLS RREGTDLFLK SGARALDSKC KAENVPGQHG QRRGRLSDYG LQLREKQKVR RIYGVLERQF RGYYQEASRR KGSTGENLL QLLECRLDNV VYRMGFGSTR SESRQLVSHK AITVNGQTVN IPSYQVKAGD VVAVREKSKN QLRIAQALEL C GQRGRVEW ...String:
RYIGPKCKLS RREGTDLFLK SGARALDSKC KAENVPGQHG QRRGRLSDYG LQLREKQKVR RIYGVLERQF RGYYQEASRR KGSTGENLL QLLECRLDNV VYRMGFGSTR SESRQLVSHK AITVNGQTVN IPSYQVKAGD VVAVREKSKN QLRIAQALEL C GQRGRVEW VEVDLDKKAG TFKSAPARSD LSADINENLI VELYSK

+
Macromolecule #37: 30S ribosomal protein S5

MacromoleculeName: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 15.761337 KDa
SequenceString:
GYIEKLVQVN RVAKTVKGGR IFAFTALTVV GDGKGRVGFG RGKAREVPAA IQKAMEAARR NMIQVDLNGT TLQYPTKSAH GASKVYMQP ASEGTGIIAG GAMRAVLEVA GVQNVLAKCY GSTNPVNVVY ATFKGLKNMQ APEAVAAKRG K

+
Macromolecule #38: 30S ribosomal protein S6

MacromoleculeName: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 11.613011 KDa
SequenceString:
RHYEIVFLVH PDQSEQVGGM VERYTKAIEE DGGKIHRLED WGRRQLAYAI NNVHKAHYVL MNVECSAKAL AELEDNFRAN DAVIRNLVM RRDEAVTEQS E

+
Macromolecule #39: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 17.313199 KDa
SequenceString:
RRRVAAKREV LADPKYGSQI LAKFMNHVME SGKKAVAERI VYGALDKVKE RGKADPLETF EKALDAIAPL VEVKSRRVGG ATYQVPVEV RPSRRNALAM RWLVDFARKR GEKSMALRLA GELLDAAEGK GAAVKKREDV HRMAEANKAF SHYRF

+
Macromolecule #40: 30S ribosomal protein S8

MacromoleculeName: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 14.001312 KDa
SequenceString:
SMQDPLADML TRIRNAQMAE KTVVSMPSSK LKAAVAKVLK DEGYIADFQI SSEVKPQLSI ELKYFEGKPV IEEVKRISRP GLRQYKSVE QLPAVRGGLG VSIVSTNKGV MTDRAARAAG VGGEVLCTVF

+
Macromolecule #41: 30S ribosomal protein S9

MacromoleculeName: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 14.139195 KDa
SequenceString:
ATQNYGTGRR KTATARVFLR PGTGKISINN RGLDQFFGRE TARMVVRQPL ELTETVEKFD IFVTVVGGGV SGQAGAIRHG ITRALIEYD ETLRSSLRKA GYVTRDAREV ERKKVGLRKA RKRPQYS

+
Macromolecule #42: 30S ribosomal protein S10

MacromoleculeName: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 10.886569 KDa
SequenceString:
IRLKAFDHRL IDQSTQEIVE TAKRTGAQVR GPIPLPTRKE RFTVLISPHV NKDARDQYEI RTHKRVLDIV QPTDKTVDAL MKLDLAAGV EVQISLG

+
Macromolecule #43: 30S ribosomal protein S11

MacromoleculeName: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 12.086657 KDa
SequenceString:
KTVVDGIAHI HASFNNTIVT ITDRQGNALS WATSGGSGFR GSRKSTPFAA QVAAERAGQA ALEYGLKNLD VNVKGPGPGR ESAVRALNA CGYKIASITD VTPIPHNGCR PPKKRR

+
Macromolecule #44: 30S ribosomal protein S12

MacromoleculeName: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 13.472612 KDa
SequenceString:
ATINQLVRKP RKRMVDKSDV PALQNCPQRR GVCTRVYTTT PKKPNSALRK VCRVRLTNGF EVSSYIGGEG HNLQEHSVVL IRGGRVKDL PGVRYHTVRG SLDTSGVKDR KQGRSKYGAK R

+
Macromolecule #45: 30S ribosomal protein S13

MacromoleculeName: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 12.307191 KDa
SequenceString:
RIAGVNIPDN KHTVISLTYI YGVGRTTAQS ICAATGVNPA AKIKDLSDEQ IDQLRNEVAK ITTEGDLRRE INMNIKRLMD LGCYRGLRH RRGLPVRGQR TKTNARTRKG P

+
Macromolecule #46: 30S ribosomal protein S14

MacromoleculeName: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 11.203024 KDa
SequenceString:
KESMKNRELK RQLTVAKYAK KRAELKAIIA NPNSSAEERW NAQVALQKQP RDASASRLRN RCRLTGRPHG FYRKFGLSRN KLREAAMRG DVPGLVKAS

+
Macromolecule #47: 30S ribosomal protein S15

MacromoleculeName: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 9.847271 KDa
SequenceString:
ALSVEEKAQI VNEYKQAEGD TGSPEVQVAL LSANINKLQD HFKANGKDHH SRRGLIRMVN QRRKLLDYLK GKDVSRYTAL IGRLGLR

+
Macromolecule #48: 30S ribosomal protein S16

MacromoleculeName: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 8.762938 KDa
SequenceString:
MVTIRLARGG SKKRPFYHLT VTNSRNARDG RFVERIGFFN PVATGGEVRL SVDQERATYW LGQGAQPSER VAQLLKDA

+
Macromolecule #49: 30S ribosomal protein S17

MacromoleculeName: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 8.848358 KDa
SequenceString:
RTLTGRVVSD KMDKTVTVLI ERRVKHPIYG KYVKRSTKLH AHDESNQCRI GDLVTIRETR PLAKTKAWTL VDIVER

+
Macromolecule #50: 30S ribosomal protein S18

MacromoleculeName: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 6.41141 KDa
SequenceString:
KEIDYKDLNT LKAYVSETGK IVPSRITGTK AKYQRQLATA IKRARYLALL PYTDSH

+
Macromolecule #51: 30S ribosomal protein S19

MacromoleculeName: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 9.182882 KDa
SequenceString:
PRSLKKGPFI DLHLLKKVEV AVEKNDRKPI KTWSRRSMIL PHMVGLTIAV HNGRQHVPVL VNEDMVGHKL GEFAATRTYR

+
Macromolecule #52: 30S ribosomal protein S20

MacromoleculeName: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 9.49714 KDa
SequenceString:
ANTPSAKKRA KQAEKRRSHN ASLRSMVRTY IKNVVKAIDA KDLEKAQAAF TAAVPVIDRM ADKGIIHKNK AARHKSRLSG HIKALS

+
Macromolecule #53: 30S ribosomal protein S21

MacromoleculeName: 30S ribosomal protein S21 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudomonas aeruginosa (bacteria)
Molecular weightTheoretical: 4.209891 KDa
SequenceString:
SREFYEKPTA ERRRKAAAAV KRHAKKVQRE QRRR

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Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 1.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: Gctf
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

4lf4

Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION
Final 3D classificationSoftware - Name: RELION
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.89 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION / Number images used: 319022

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