+
データを開く
-
基本情報
登録情報 | データベース: EMDB / ID: EMD-10068 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
タイトル | Cryo-EM structures of Lsg1-TAP pre-60S ribosomal particles | |||||||||
![]() | ||||||||||
![]() |
| |||||||||
![]() | 60S subunit / eIF6 / Nmd3 / Lsg1 / RIBOSOME | |||||||||
機能・相同性 | ![]() budding cell bud growth / protein tyrosine/serine/threonine phosphatase activity / ascospore wall assembly / 加水分解酵素 / preribosome binding / positive regulation of autophagosome assembly / mating projection tip / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cellular response to nitrogen starvation / nucleocytoplasmic transport ...budding cell bud growth / protein tyrosine/serine/threonine phosphatase activity / ascospore wall assembly / 加水分解酵素 / preribosome binding / positive regulation of autophagosome assembly / mating projection tip / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cellular response to nitrogen starvation / nucleocytoplasmic transport / pre-mRNA 5'-splice site binding / maturation of 5.8S rRNA / response to cycloheximide / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / ribosomal large subunit binding / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / negative regulation of mRNA splicing, via spliceosome / Formation of a pool of free 40S subunits / preribosome, large subunit precursor / L13a-mediated translational silencing of Ceruloplasmin expression / 加水分解酵素; 酸無水物に作用; リン含有酸無水物に作用 / translational elongation / ribosomal large subunit export from nucleus / ribosomal subunit export from nucleus / regulation of translational fidelity / protein-RNA complex assembly / maturation of LSU-rRNA / translation initiation factor activity / Neutrophil degranulation / protein tyrosine phosphatase activity / protein-tyrosine-phosphatase / protein tyrosine phosphatase activity, metal-dependent / histone H2AXY142 phosphatase activity / non-membrane spanning protein tyrosine phosphatase activity / assembly of large subunit precursor of preribosome / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cytosolic ribosome assembly / ribosomal large subunit biogenesis / meiotic cell cycle / translational initiation / macroautophagy / maintenance of translational fidelity / autophagy / cytoplasmic stress granule / metallopeptidase activity / rRNA processing / protein transport / mitotic cell cycle / ribosome biogenesis / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / protein-macromolecule adaptor activity / cytosolic large ribosomal subunit / sequence-specific DNA binding / cytoplasmic translation / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / response to antibiotic / GTPase activity / mRNA binding / GTP binding / nucleolus / proteolysis / RNA binding / zinc ion binding / nucleoplasm / metal ion binding / nucleus / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.2 Å | |||||||||
![]() | Kargas V / Warren AJ | |||||||||
資金援助 | ![]()
| |||||||||
![]() | ![]() タイトル: Mechanism of completion of peptidyltransferase centre assembly in eukaryotes. 著者: Vasileios Kargas / Pablo Castro-Hartmann / Norberto Escudero-Urquijo / Kyle Dent / Christine Hilcenko / Carolin Sailer / Gertrude Zisser / Maria J Marques-Carvalho / Simone Pellegrino / ...著者: Vasileios Kargas / Pablo Castro-Hartmann / Norberto Escudero-Urquijo / Kyle Dent / Christine Hilcenko / Carolin Sailer / Gertrude Zisser / Maria J Marques-Carvalho / Simone Pellegrino / Leszek Wawiórka / Stefan Mv Freund / Jane L Wagstaff / Antonina Andreeva / Alexandre Faille / Edwin Chen / Florian Stengel / Helmut Bergler / Alan John Warren / ![]() ![]() ![]() ![]() 要旨: During their final maturation in the cytoplasm, pre-60S ribosomal particles are converted to translation-competent large ribosomal subunits. Here, we present the mechanism of peptidyltransferase ...During their final maturation in the cytoplasm, pre-60S ribosomal particles are converted to translation-competent large ribosomal subunits. Here, we present the mechanism of peptidyltransferase centre (PTC) completion that explains how integration of the last ribosomal proteins is coupled to release of the nuclear export adaptor Nmd3. Single-particle cryo-EM reveals that eL40 recruitment stabilises helix 89 to form the uL16 binding site. The loading of uL16 unhooks helix 38 from Nmd3 to adopt its mature conformation. In turn, partial retraction of the L1 stalk is coupled to a conformational switch in Nmd3 that allows the uL16 P-site loop to fully accommodate into the PTC where it competes with Nmd3 for an overlapping binding site (base A2971). Our data reveal how the central functional site of the ribosome is sculpted and suggest how the formation of translation-competent 60S subunits is disrupted in leukaemia-associated ribosomopathies. | |||||||||
履歴 |
|
-
構造の表示
ムービー |
![]() |
---|---|
構造ビューア | EMマップ: ![]() ![]() ![]() |
添付画像 |
-
ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 18.3 MB | ![]() | |
---|---|---|---|---|
ヘッダ (付随情報) | ![]() ![]() | 65.6 KB 65.6 KB | 表示 表示 | ![]() |
画像 | ![]() | 75.7 KB | ||
Filedesc metadata | ![]() | 14.7 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 6rzzMC ![]() 4560C ![]() 4630C ![]() 4636C ![]() 4884C ![]() 6qikC ![]() 6qt0C ![]() 6qtzC ![]() 6ri5C ![]() 6s05C C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
---|---|
類似構造データ |
-
リンク
EMDBのページ | ![]() ![]() |
---|---|
「今月の分子」の関連する項目 |
-
マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.065 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
|
-添付データ
-
試料の構成要素
+全体 : Lsg1-TAP 60s ribosomal subunit
+超分子 #1: Lsg1-TAP 60s ribosomal subunit
+分子 #1: 25S ribosomal RNA
+分子 #47: 5S ribosomal RNA
+分子 #48: 5.8S ribosomal RNA
+分子 #2: 60S ribosomal protein L2-A
+分子 #3: 60S ribosomal protein L3
+分子 #4: 60S ribosomal protein L4-A
+分子 #5: 60S ribosomal protein L11-A
+分子 #6: 60S ribosomal protein L9-A
+分子 #7: 60S ribosomal protein L6-A
+分子 #8: 60S ribosomal protein L8-A
+分子 #9: 60S ribosomal protein L16-B
+分子 #10: 60S ribosomal protein L13-A
+分子 #11: 60S ribosomal protein L23-A
+分子 #12: 60S ribosomal protein L14-A
+分子 #13: 60S ribosomal protein L28
+分子 #14: 60S ribosomal protein L15-A
+分子 #15: 60S ribosomal protein L5
+分子 #16: 60S ribosomal protein L18-A
+分子 #17: 60S ribosomal protein L19-A
+分子 #18: 60S ribosomal protein L20-A
+分子 #19: 60S ribosomal protein L21-A
+分子 #20: 60S ribosomal protein L17-A
+分子 #21: 60S ribosomal protein L22-A
+分子 #22: 60S ribosomal protein L25
+分子 #23: 60S ribosomal protein L26-A
+分子 #24: 60S ribosomal protein L27-A
+分子 #25: 60S ribosomal protein L35-A
+分子 #26: 60S ribosomal protein L29
+分子 #27: 60S ribosomal protein L7-A
+分子 #28: 60S ribosomal protein L30
+分子 #29: 60S ribosomal protein L31-A
+分子 #30: 60S ribosomal protein L32
+分子 #31: 60S ribosomal protein L33-A
+分子 #32: 60S ribosomal protein L34-A
+分子 #33: 60S ribosomal protein L36-A
+分子 #34: 60S ribosomal protein L37-A
+分子 #35: 60S ribosomal protein L38
+分子 #36: 60S ribosomal protein L39
+分子 #37: 60S ribosomal protein L42-A
+分子 #38: 60S ribosomal protein L43-A
+分子 #39: Eukaryotic translation initiation factor 6
+分子 #40: Large subunit GTPase 1
+分子 #41: uL1
+分子 #42: Probable metalloprotease ARX1
+分子 #43: Tyrosine-protein phosphatase YVH1
+分子 #44: Cytoplasmic 60S subunit biogenesis factor REI1
+分子 #45: 60S ribosomal protein L24-A
+分子 #46: 60S ribosomal export protein NMD3
+分子 #49: ZINC ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
---|---|
![]() | 単粒子再構成法 |
試料の集合状態 | particle |
-
試料調製
緩衝液 | pH: 7.5 |
---|---|
凍結 | 凍結剤: ETHANE |
-
電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
---|---|
撮影 | フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 平均電子線量: 63.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
-
画像解析
初期モデル | モデルのタイプ: OTHER |
---|---|
最終 再構成 | 想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 3.2 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION (ver. 2.1) / 使用した粒子像数: 216403 |
初期 角度割当 | タイプ: ANGULAR RECONSTITUTION |
最終 角度割当 | タイプ: ANGULAR RECONSTITUTION |