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- EMDB-0661: 70S Elongation Competent Ribosome -

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Basic information

Entry
Database: EMDB / ID: EMD-0661
Title70S Elongation Competent Ribosome
Map data
Sample70S elongation competent ribosome:
(nucleic-acidNucleic acid) x 4 / (50S ribosomal protein ...) x 29 / (30S ribosomal protein ...) x 20
Function / homology
Function and homology information


ribosomal small subunit assembly / small ribosomal subunit rRNA binding / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / mRNA 5'-UTR binding / small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / large ribosomal subunit / cytosolic small ribosomal subunit / ribosomal large subunit assembly ...ribosomal small subunit assembly / small ribosomal subunit rRNA binding / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / mRNA 5'-UTR binding / small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / large ribosomal subunit / cytosolic small ribosomal subunit / ribosomal large subunit assembly / 5S rRNA binding / transferase activity / tRNA binding / ribosome / rRNA binding / structural constituent of ribosome / translation / mRNA binding / metal ion binding / cytosol
Ribosomal protein S8 superfamily / Ribosomal protein S11 superfamily / Ribosomal Protein L26/L24, KOW domain / Ribosomal protein L19 superfamily / Ribosomal protein L33 superfamily / Ribosomal protein S21 superfamily / Ribosomal protein L35 superfamily / RNA-binding S4 domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein S4/S9 N-terminal domain ...Ribosomal protein S8 superfamily / Ribosomal protein S11 superfamily / Ribosomal Protein L26/L24, KOW domain / Ribosomal protein L19 superfamily / Ribosomal protein L33 superfamily / Ribosomal protein S21 superfamily / Ribosomal protein L35 superfamily / RNA-binding S4 domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein S4/S9 N-terminal domain / Ribosomal protein L10e/L16 superfamily / Ribosomal protein L30, ferredoxin-like fold domain superfamily / Ribosomal protein L13 superfamily / Ribosomal protein S18 superfamily / Ribosomal protein L14 superfamily / Ribosomal protein S10 domain superfamily / Ribosomal protein S7 domain superfamily / Ribosomal protein L31 superfamily / Ribosomal protein S12/S23 / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L5 / Ribosomal protein S5, N-terminal domain / Ribosomal protein L30p/L7e / Ribosomal protein S2 / Ribosomal protein S15 / Ribosomal protein L11, RNA binding domain / Ribosomal protein L3 / Ribosomal protein L23 / Ribosomal protein S7p/S5e / Ribosomal protein S14p/S29e / Ribosomal protein L16p/L10e / Ribosomal protein L14p/L23e / Ribosomal protein L22p/L17e / Ribosomal protein S19 / Ribosomal protein S3, C-terminal domain / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L6, alpha-beta domain superfamily / Ribosomal protein L6 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S7 domain / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S19, superfamily / Ribosomal protein L4 domain superfamily / Ribosomal protein L13, conserved site / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L5 domain superfamily / 30s ribosomal protein S13, C-terminal / Ribosomal protein S4/S9 / Ribosomal protein L2, conserved site / Ribosomal protein L2, C-terminal / Ribosomal Proteins L2, RNA binding domain / Ribosomal protein L35 / Ribosomal protein L18e/L15P / Ribosomal protein L34, conserved site / Ribosomal protein S16 domain superfamily / Ribosomal protein S10 domain / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein S6 superfamily / Ribosomal protein S20 superfamily / Ribosomal protein S3, C-terminal domain superfamily / Ribosomal protein L22/L17 superfamily / Ribosomal protein L17 superfamily / Ribosomal L18e/L15P superfamily / L21-like superfamily / Ribosomal protein L29/L35 superfamily / Ribosomal protein L36 superfamily / Ribosomal protein L31 type A / Ribosomal protein L20, C-terminal / L28p-like / Ribosomal protein L5, N-terminal / Ribosomal protein L5, C-terminal / Ribosomal protein L15 / Ribosomal protein L25 / Ribosomal protein L21-like / Ribosomal protein S10p/S20e / Ribosomal protein S17 / Ribosomal protein L19 signature. / Ribosomal protein S10 signature. / Ribosomal protein L6 signature 1. / Ribosomal protein L15 signature. / Ribosomal protein L3 signature. / Ribosomal protein L2 signature. / Ribosomal protein L22 signature. / Ribosomal protein S15 signature. / Ribosomal protein S9 signature. / Ribosomal protein S3 signature. / Ribosomal protein L11 signature. / Ribosomal protein L5 signature. / Ribosomal protein S19 signature. / Ribosomal protein S18 signature. / Ribosomal protein S17 signature. / Ribosomal protein S12 signature. / Ribosomal protein S11 signature. / Ribosomal protein S14 signature. / Ribosomal protein L29 signature. / Ribosomal protein S7 signature. / Ribosomal protein L13 signature. / Ribosomal protein S2 signature 1. / Ribosomal protein L20 signature.
50S ribosomal protein L17 / 50S ribosomal protein L19 / 50S ribosomal protein L35 / 50S ribosomal protein L2 / 50S ribosomal protein L31 / 30S ribosomal protein S19 / 30S ribosomal protein S8 / 50S ribosomal protein L9 / 30S ribosomal protein S18 / 50S ribosomal protein L6 ...50S ribosomal protein L17 / 50S ribosomal protein L19 / 50S ribosomal protein L35 / 50S ribosomal protein L2 / 50S ribosomal protein L31 / 30S ribosomal protein S19 / 30S ribosomal protein S8 / 50S ribosomal protein L9 / 30S ribosomal protein S18 / 50S ribosomal protein L6 / 30S ribosomal protein S6 / 30S ribosomal protein S4 / 30S ribosomal protein S11 / 50S ribosomal protein L16 / 50S ribosomal protein L3 / 50S ribosomal protein L18 / 50S ribosomal protein L20 / 30S ribosomal protein S20 / 30S ribosomal protein S9 / 30S ribosomal protein S2 / 50S ribosomal protein L13 / 50S ribosomal protein L5 / 50S ribosomal protein L21 / 50S ribosomal protein L27 / 50S ribosomal protein L29 / 50S ribosomal protein L14 / 50S ribosomal protein L30 / 50S ribosomal protein L15 / 50S ribosomal protein L32 / 30S ribosomal protein S17 / 30S ribosomal protein S14 / 50S ribosomal protein L23 / 50S ribosomal protein L36 / 50S ribosomal protein L24 / 50S ribosomal protein L11 / 30S ribosomal protein S21 / 30S ribosomal protein S13 / 50S ribosomal protein L22 / 30S ribosomal protein S7 / 30S ribosomal protein S3 / 30S ribosomal protein S5 / 50S ribosomal protein L34 / 30S ribosomal protein S16 / 30S ribosomal protein S15 / 50S ribosomal protein L33 / 30S ribosomal protein S10 / 50S ribosomal protein L25 / 50S ribosomal protein L4 / 30S ribosomal protein S12
Biological speciesEscherichia coli (E. coli) / Escherichia coli
Methodsingle particle reconstruction / cryo EM / Resolution: 3.9 Å
AuthorsFrank J / Gonzalez Jr RL / Kaledhonkar S / Fu Z / Caban K / Li W / Chen B / Sun M
CitationJournal: Nature / Year: 2019
Title: Late steps in bacterial translation initiation visualized using time-resolved cryo-EM.
Authors: Sandip Kaledhonkar / Ziao Fu / Kelvin Caban / Wen Li / Bo Chen / Ming Sun / Ruben L Gonzalez / Joachim Frank /
Abstract: The initiation of bacterial translation involves the tightly regulated joining of the 50S ribosomal subunit to an initiator transfer RNA (fMet-tRNA)-containing 30S ribosomal initiation complex to ...The initiation of bacterial translation involves the tightly regulated joining of the 50S ribosomal subunit to an initiator transfer RNA (fMet-tRNA)-containing 30S ribosomal initiation complex to form a 70S initiation complex, which subsequently matures into a 70S elongation-competent complex. Rapid and accurate formation of the 70S initiation complex is promoted by initiation factors, which must dissociate from the 30S initiation complex before the resulting 70S elongation-competent complex can begin the elongation of translation. Although comparisons of the structures of the 30S and 70S initiation complexes have revealed that the ribosome, initiation factors and fMet-tRNA can acquire different conformations in these complexes, the timing of conformational changes during formation of the 70S initiation complex, the structures of any intermediates formed during these rearrangements, and the contributions that these dynamics might make to the mechanism and regulation of initiation remain unknown. Moreover, the absence of a structure of the 70S elongation-competent complex formed via an initiation-factor-catalysed reaction has precluded an understanding of the rearrangements to the ribosome, initiation factors and fMet-tRNA that occur during maturation of a 70S initiation complex into a 70S elongation-competent complex. Here, using time-resolved cryogenic electron microscopy, we report the near-atomic-resolution view of how a time-ordered series of conformational changes drive and regulate subunit joining, initiation factor dissociation and fMet-tRNA positioning during formation of the 70S elongation-competent complex. Our results demonstrate the power of time-resolved cryogenic electron microscopy to determine how a time-ordered series of conformational changes contribute to the mechanism and regulation of one of the most fundamental processes in biology.
Validation ReportPDB-ID: 6o9j

SummaryFull reportAbout validation report
DateDeposition: Mar 14, 2019 / Header (metadata) release: May 29, 2019 / Map release: May 29, 2019 / Update: May 29, 2019

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.04
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  • Surface view colored by height
  • Surface level: 0.04
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: : PDB-6o9j
  • Surface level: 0.04
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_0661.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.66 Å/pix.
x 256 pix.
= 424.96 Å
1.66 Å/pix.
x 256 pix.
= 424.96 Å
1.66 Å/pix.
x 256 pix.
= 424.96 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.66 Å
Density
Contour LevelBy AUTHOR: 0.03 / Movie #1: 0.04
Minimum - Maximum-0.14872783 - 0.30176935
Average (Standard dev.)0.0015355911 (±0.012583342)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 424.96 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.661.661.66
M x/y/z256256256
origin x/y/z0.0000.0000.000
length x/y/z424.960424.960424.960
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS256256256
D min/max/mean-0.1490.3020.002

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Supplemental data

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Sample components

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Entire 70S elongation competent ribosome

EntireName: 70S elongation competent ribosome / Number of components: 54

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Component #1: protein, 70S elongation competent ribosome

ProteinName: 70S elongation competent ribosome / Recombinant expression: No
SourceSpecies: Escherichia coli (E. coli)

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Component #2: nucleic-acid, 5S rRNA

nucleic acidName: 5S rRNA5S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GCCUGGCGGC CGUAGCGCGG UGGUCCCACC UGACCCCAUG CCGAACUCAG AAGUGAAACG CCGUAGCGCC GAUGGUAGUG UGGGGUCUCC CCAUGCGAGA GUAGGGAACU GCCAGGC
MassTheoretical: 37.848555 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #3: nucleic-acid, 23S rRNA

nucleic acidName: 23S rRNA23S ribosomal RNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GGUUAAGCGA CUAAGCGUAC ACGGUGGAUG CCCUGGCAGU CAGAGGCGAU GAAGGACGUG CUAAUCUGCG AUAAGCGUCG GUAAGGUGAU AUGAACCGUU AUAACCGGCG AUUUCCGAAU GGGGAAACCC AGUGUGUUUC GACACACUAU CAUUAACUGA AUCCAUAGGU UAAUGAGGCG AACCGGGGGA ACUGAAACAU CUAAGUACCC CGAGGAAAAG AAAUCAACCG AGAUUCCCCC AGUAGCGGCG AGCGAACGGG GAGCAGCCCA GAGCCUGAAU CAGUGUGUGU GUUAGUGGAA GCGUCUGGAA AGGCGCGCGA UACAGGGUGA CAGCCCCGUA CACAAAAAUG CACAUGCUGU GAGCUCGAUG AGUAGGGCGG GACACGUGGU AUCCUGUCUG AAUAUGGGGG GACCAUCCUC CAAGGCUAAA UACUCCUGAC UGACCGAUAG UGAACCAGUA CCGUGAGGGA AAGGCGAAAA GAACCCCGGC GAGGGGAGUG AAAAAGAACC UGAAACCGUG UACGUACAAG CAGUGGGAGC ACGCUUAGGC GUGUGACUGC GUACCUUUUG UAUAAUGGGU CAGCGACUUA UAUUCUGUAG CAAGGUUAAC CGAAUAGGGG AGCCGAAGGG AAACCGAGUC UUAACUGGGC GUUAAGUUGC AGGGUAUAGA CCCGAAACCC GGUGAUCUAG CCAUGGGCAG GUUGAAGGUU GGGUAACACU AACUGGAGGA CCGAACCGAC UAAUGUUGAA AAAUUAGCGG AUGACUUGUG GCUGGGGGUG AAAGGCCAAU CAAACCGGGA GAUAGCUGGU UCUCCCCGAA AGCUAUUUAG GUAGCGCCUC GUGAAUUCAU CUCCGGGGGU AGAGCACUGU UUCGGCAACA ACCCGAUGCA AACUGCGAAU ACCGGAGAAU GUUAUCACGG GAGACACACG GCGGGUGCUA ACGUCCGUCG UGAAGAGGGA AACAACCCAG ACCGCCAGCU AAGGUCCCAA AGUCAUGGUU AAGUGGGAAA CGAUGUGGGA AGGCCCAGAC AGCCAGGAUG UUGGCUUAGA AGCAGCCAUC AUUUAAAGAA AGCGUAAUAG CUCACUGGUC GAGUCGGCCU GCGCGGAAGA UGUAACGGGG CUAAACCAUG CACCGAAGCU GCGGCAGCGA CGCUUAUGCG UUGUUGGGUA GGGGAGCGUU CUGUAAGCCU GCGAAGGUGU GCUGUGAGGC AUGCUGGAGG UAUCAGAAGU GCGAAUGCUG ACAUAAGUAA CGAUAAAGCG GGUGAAAAGC CCGCUCGCCG GAAGACCAAG GGUUCCUGUC CAACGUUAAU CGGGGCAGGG UGAGUCGACC CCUAAGGCGA GGCCGAAAGG CGUAGUCGAU GGGAAACAGG UUAAUAUUCC UGUACUUGGU GUUACUGCGA AGGGGGGACG GAGAAGGCUA UGUUGGCCGG GCGACGGUUG UCCCGGUUUA AGCGUGUAGG CUGGUUUUCC AGGCAAAUCC GGAAAAUCAA GGCUGAGGCG UGAUGACGAG GCACUACGGU GCUGAAGCAA CAAAUGCCCU GCUUCCAGGA AAAGCCUCUA AGCAUCAGGU AACAUCAAAU CGUACCCCAA ACCGACACAG GUGGUCAGGU AGAGAAUACC AAGGCGCUUG AGAGAACUCG GGUGAAGGAA CUAGGCAAAA UGGUGCCGUA ACUUCGGGAG AAGGCACGCU GAUAUGUAGG UGAGGUCCCU CGCGGAUGGA GCUGAAAUCA GUCGAAGAUA CCAGCUGGCU GCAACUGUUU AUUAAAAACA CAGCACUGUG CAAACACGAA AGUGGACGUA UACGGUGUGA CGCCUGCCCG GUGCCGGAAG GUUAAUUGAU GGGGUUAGCG CAAGCGAAGC UCUUGAUCGA AGCCCCGGUA AACGGCGGCC GUAACUAUAA CGGUCCUAAG GUAGCGAAAU UCCUUGUCGG GUAAGUUCCG ACCUGCACGA AUGGCGUAAU GAUGGCCAGG CUGUCUCCAC CCGAGACUCA GUGAAAUUGA ACUCGCUGUG AAGAUGCAGU GUACCCGCGG CAAGACGGAA AGACCCCGUG AACCUUUACU AUAGCUUGAC ACUGAACAUU GAGCCUUGAU GGAAGUGUGG ACGCCAGUCU GCAUGGCUUU AAUGUUUGAU GUUCUAACGU UGACCCGUAA UCCGGGUUGC GGACAGUGUC UGGUGGGUAG UUUGACUGGG GCGGUCUCCU CCUAAAGAGU AACGGAGGAG CACGAAGGUU GGCUAAUCCU GGUCGGACAU CAGGAGGUUA GUGCAAUGGC AUAAGCCAGC UUGACUGCGA GCGUGACGGC GCGAGCAGGU GCGAAAGCAG GUCAUAGUGA UCCGGUGGUU CUGAAUGGAA GGGCCAUCGC UCAACGGAUA AAAGGUACUC CGGGGAUAAC AGGCUGAUAC CGCCCAAGAG UUCAUAUCGA CGGCGGUGUU UGGCACCUCG AUGUCGGCUC AUCACAUCCU GGGGCUGAAG UAGGUCCCAA GGGUAUGGCU GUUCGCCAUU UAAAGUGGUA CGCGAGCUGG GUUUAGAACG UCGUGAGACA GUUCGGUCCC UAUCUGCCGU GGGCGCUGGA GAACUGAGGG GGGCUGCUCC UAGUACGAGA GGACCGGAGU GGACGCAUCA CUGGUGUUCG GGUUGUCAUG CCAAUGGCAC UGCCCGGUAG CUAAAUGCGG AAGAGAUAAG UGCUGAAAGC AUCUAAGCAC GAAACUUGCC CCGAGAUGAG UUCUCCCUGA CCCUUUAAGG GUCCUGAAGG AACGUUGAAG ACGACGACGU UGAUAGGCCG GGUGUGUAAG CGCAGCGAUG CGUUGAGCUA ACCGGUACUA AUGAACCGUG AGGCUUAACC U
MassTheoretical: 921.273375 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #4: protein, 50S ribosomal protein L25

ProteinName: 50S ribosomal protein L25 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.713465 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #5: protein, 50S ribosomal protein L2

ProteinName: 50S ribosomal protein L2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.248758 kDa
SourceSpecies: Escherichia coli

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Component #6: protein, 50S ribosomal protein L3

ProteinName: 50S ribosomal protein L3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.277535 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #7: protein, 50S ribosomal protein L4

ProteinName: 50S ribosomal protein L4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 22.121566 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #8: protein, 50S ribosomal protein L5

ProteinName: 50S ribosomal protein L5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 20.202416 kDa
SourceSpecies: Escherichia coli

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Component #9: protein, 50S ribosomal protein L6

ProteinName: 50S ribosomal protein L6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 18.801598 kDa
SourceSpecies: Escherichia coli

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Component #10: protein, 50S ribosomal protein L9

ProteinName: 50S ribosomal protein L9 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.78902 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #11: protein, 50S ribosomal protein L13

ProteinName: 50S ribosomal protein L13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.82236 kDa
SourceSpecies: Escherichia coli

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Component #12: protein, 50S ribosomal protein L14

ProteinName: 50S ribosomal protein L14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.320714 kDa
SourceSpecies: Escherichia coli

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Component #13: protein, 50S ribosomal protein L15

ProteinName: 50S ribosomal protein L15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.008471 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #14: protein, 50S ribosomal protein L16

ProteinName: 50S ribosomal protein L16 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.312269 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #15: protein, 50S ribosomal protein L17

ProteinName: 50S ribosomal protein L17 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.393657 kDa
SourceSpecies: Escherichia coli

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Component #16: protein, 50S ribosomal protein L18

ProteinName: 50S ribosomal protein L18 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.794668 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #17: protein, 50S ribosomal protein L19

ProteinName: 50S ribosomal protein L19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.028082 kDa
SourceSpecies: Escherichia coli

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Component #18: protein, 50S ribosomal protein L20

ProteinName: 50S ribosomal protein L20 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.396828 kDa
SourceSpecies: Escherichia coli

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Component #19: protein, 50S ribosomal protein L21

ProteinName: 50S ribosomal protein L21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.586374 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #20: protein, 50S ribosomal protein L22

ProteinName: 50S ribosomal protein L22 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.253359 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #21: protein, 50S ribosomal protein L23

ProteinName: 50S ribosomal protein L23 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.093047 kDa
SourceSpecies: Escherichia coli

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Component #22: protein, 50S ribosomal protein L24

ProteinName: 50S ribosomal protein L24 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.078874 kDa
SourceSpecies: Escherichia coli

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Component #23: protein, 50S ribosomal protein L27

ProteinName: 50S ribosomal protein L27 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.015344 kDa
SourceSpecies: Escherichia coli

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Component #24: protein, 50S ribosomal protein L29

ProteinName: 50S ribosomal protein L29 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.286464 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #25: protein, 50S ribosomal protein L30

ProteinName: 50S ribosomal protein L30 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.423625 kDa
SourceSpecies: Escherichia coli

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Component #26: protein, 50S ribosomal protein L31

ProteinName: 50S ribosomal protein L31 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.887117 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #27: protein, 50S ribosomal protein L32

ProteinName: 50S ribosomal protein L32 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.332249 kDa
SourceSpecies: Escherichia coli

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Component #28: protein, 50S ribosomal protein L33

ProteinName: 50S ribosomal protein L33 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.257436 kDa
SourceSpecies: Escherichia coli

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Component #29: protein, 50S ribosomal protein L34

ProteinName: 50S ribosomal protein L34 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 5.397463 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #30: protein, 50S ribosomal protein L35

ProteinName: 50S ribosomal protein L35 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.181835 kDa
SourceSpecies: Escherichia coli

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Component #31: protein, 50S ribosomal protein L36

ProteinName: 50S ribosomal protein L36 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 4.37739 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #32: protein, 50S ribosomal protein L11

ProteinName: 50S ribosomal protein L11 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.763165 kDa
SourceSpecies: Escherichia coli

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Component #33: nucleic-acid, 16S rRNA

nucleic acidName: 16S rRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
UGAAGAGUUU GAUCAUGGCU CAGAUUGAAC GCUGGCGGCA GGCCUAACAC AUGCAAGUCG AACGGUAACA GGAAGAAGCU UGCUUCUUUG CUGACGAGUG GCGGACGGGU GAGUAAUGUC UGGGAAACUG CCUGAUGGAG GGGGAUAACU ACUGGAAACG GUAGCUAAUA CCGCAUAACG UCGCAAGACC AAAGAGGGGG ACCUUCGGGC CUCUUGCCAU CGGAUGUGCC CAGAUGGGAU UAGCUAGUAG GUGGGGUAAC GGCUCACCUA GGCGACGAUC CCUAGCUGGU CUGAGAGGAU GACCAGCCAC ACUGGAACUG AGACACGGUC CAGACUCCUA CGGGAGGCAG CAGUGGGGAA UAUUGCACAA UGGGCGCAAG CCUGAUGCAG CCAUGCCGCG UGUAUGAAGA AGGCCUUCGG GUUGUAAAGU ACUUUCAGCG GGGAGGAAGG GAGUAAAGUU AAUACCUUUG CUCAUUGACG UUACCCGCAG AAGAAGCACC GGCUAACUCC GUGCCAGCAG CCGCGGUAAU ACGGAGGGUG CAAGCGUUAA UCGGAAUUAC UGGGCGUAAA GCGCACGCAG GCGGUUUGUU AAGUCAGAUG UGAAAUCCCC GGGCUCAACC UGGGAACUGC AUCUGAUACU GGCAAGCUUG AGUCUCGUAG AGGGGGGUAG AAUUCCAGGU GUAGCGGUGA AAUGCGUAGA GAUCUGGAGG AAUACCGGUG GCGAAGGCGG CCCCCUGGAC GAAGACUGAC GCUCAGGUGC GAAAGCGUGG GGAGCAAACA GGAUUAGAUA CCCUGGUAGU CCACGCCGUA AACGAUGUCG ACUUGGAGGU UGUGCCCUUG AGGCGUGGCU UCCGGAGCUA ACGCGUUAAG UCGACCGCCU GGGGAGUACG GCCGCAAGGU UAAAACUCAA AUGAAUUGAC GGGGGCCCGC ACAAGCGGUG GAGCAUGUGG UUUAAUUCGA UGCAACGCGA AGAACCUUAC CUGGUCUUGA CAUCCACGGA AGUUUUCAGA GAUGAGAAUG UGCCUUCGGG AACCGUGAGA CAGGUGCUGC AUGGCUGUCG UCAGCUCGUG UUGUGAAAUG UUGGGUUAAG UCCCGCAACG AGCGCAACCC UUAUCCUUUG UUGCCAGCGG UCCGGCCGGG AACUCAAAGG AGACUGCCAG UGAUAAACUG GAGGAAGGUG GGGAUGACGU CAAGUCAUCA UGGCCCUUAC GACCAGGGCU ACACACGUGC UACAAUGGCG CAUACAAAGA GAAGCGACCU CGCGAGAGCA AGCGGACCUC AUAAAGUGCG UCGUAGUCCG GAUUGGAGUC UGCAACUCGA CUCCAUGAAG UCGGAAUCGC UAGUAAUCGU GGAUCAGAAU GCCACGGUGA AUACGUUCCC GGGCCUUGUA CACACCGCCC GUCACACCAU GGGAGUGGGU UGCAAAAGAA GUAGGUAGCU UAACCUUCGG GAGGGCGCUU ACCACUUUGU GAUUCAUGAC UGGGGUGAAG UCGUAACAAG GUAACCGUAG GGGAACCUGC GGUUGGAUCA
MassTheoretical: 495.927719 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #34: protein, 30S ribosomal protein S3

ProteinName: 30S ribosomal protein S3 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.078785 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #35: protein, 30S ribosomal protein S4

ProteinName: 30S ribosomal protein S4 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.383002 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #36: protein, 30S ribosomal protein S5

ProteinName: 30S ribosomal protein S5 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 15.804282 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #37: protein, 30S ribosomal protein S6

ProteinName: 30S ribosomal protein S6 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.669371 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #38: protein, 30S ribosomal protein S7

ProteinName: 30S ribosomal protein S7 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 16.764406 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #39: protein, 30S ribosomal protein S8

ProteinName: 30S ribosomal protein S8 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.015361 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #40: protein, 30S ribosomal protein S9

ProteinName: 30S ribosomal protein S9 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 14.554882 kDa
SourceSpecies: Escherichia coli (E. coli)

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Component #41: protein, 30S ribosomal protein S10

ProteinName: 30S ribosomal protein S10 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.196988 kDa
SourceSpecies: Escherichia coli (E. coli)

+
Component #42: protein, 30S ribosomal protein S11

ProteinName: 30S ribosomal protein S11 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.4872 kDa
SourceSpecies: Escherichia coli (E. coli)

+
Component #43: protein, 30S ribosomal protein S12

ProteinName: 30S ribosomal protein S12 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 13.636961 kDa
SourceSpecies: Escherichia coli (E. coli)

+
Component #44: protein, 30S ribosomal protein S13

ProteinName: 30S ribosomal protein S13 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.625753 kDa
SourceSpecies: Escherichia coli (E. coli)

+
Component #45: protein, 30S ribosomal protein S14

ProteinName: 30S ribosomal protein S14 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 11.48939 kDa
SourceSpecies: Escherichia coli (E. coli)

+
Component #46: protein, 30S ribosomal protein S15

ProteinName: 30S ribosomal protein S15 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 10.188687 kDa
SourceSpecies: Escherichia coli (E. coli)

+
Component #47: protein, 30S ribosomal protein S16

ProteinName: 30S ribosomal protein S16 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.207572 kDa
SourceSpecies: Escherichia coli (E. coli)

+
Component #48: protein, 30S ribosomal protein S17

ProteinName: 30S ribosomal protein S17 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.263946 kDa
SourceSpecies: Escherichia coli (E. coli)

+
Component #49: protein, 30S ribosomal protein S18

ProteinName: 30S ribosomal protein S18 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.466477 kDa
SourceSpecies: Escherichia coli (E. coli)

+
Component #50: protein, 30S ribosomal protein S19

ProteinName: 30S ribosomal protein S19 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.057626 kDa
SourceSpecies: Escherichia coli (E. coli)

+
Component #51: protein, 30S ribosomal protein S20

ProteinName: 30S ribosomal protein S20 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 9.50619 kDa
SourceSpecies: Escherichia coli (E. coli)

+
Component #52: protein, 30S ribosomal protein S2

ProteinName: 30S ribosomal protein S2 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 24.253943 kDa
SourceSpecies: Escherichia coli (E. coli)

+
Component #53: protein, 30S ribosomal protein S21

ProteinName: 30S ribosomal protein S21 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 6.067081 kDa
SourceSpecies: Escherichia coli (E. coli)

+
Component #54: nucleic-acid, P-tRNA

nucleic acidName: P-tRNA / Class: RNA / Structure: OTHER / Synthetic: No
Sequence:
GCCCGGAUAG CUCAGUCGGU AGAGCAGGGG AUUGAAAAUC CCCGUGUCCU UGGUUCGAUU CCGAGUCCGG GCACCA
MassTheoretical: 24.485539 kDa
SourceSpecies: Escherichia coli (E. coli)

-
Experimental details

-
Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionpH: 7.2
Support filmunspecified
VitrificationCryogen name: ETHANE

-
Electron microscopy imaging

Experimental equipment
Model: Tecnai F30 / Image courtesy: FEI Company
ImagingMicroscope: FEI TECNAI F30
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 8 e/Å2 / Illumination mode: OTHER
LensImaging mode: DARK FIELD
Specimen HolderModel: OTHER
CameraDetector: GATAN K2 SUMMIT (4k x 4k)

-
Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 46042
3D reconstructionResolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF
FSC plot (resolution estimation)

-
Atomic model buiding

Output model

+
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