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- EMDB-0499: CryoEM structure of Helicobacter pylori urea channel in open state. -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-0499 | |||||||||
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Title | CryoEM structure of Helicobacter pylori urea channel in open state. | |||||||||
![]() | em-volume_P1.map | |||||||||
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![]() | Helicobacter pylori / urea channel / open state / TRANSPORT PROTEIN | |||||||||
Function / homology | AmiS/UreI transporter / AmiS/UreI transporter superfamily / AmiS/UreI family transporter / identical protein binding / plasma membrane / Acid-activated urea channel![]() | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.7 Å | |||||||||
![]() | Cui YX / Zhou K | |||||||||
Funding support | ![]()
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![]() | ![]() Title: pH-dependent gating mechanism of the urea channel revealed by cryo-EM. Authors: Yanxiang Cui / Kang Zhou / David Strugatsky / Yi Wen / George Sachs / Z Hong Zhou / Keith Munson / ![]() Abstract: The urea channel of (UreI) is an ideal drug target for preventing gastric cancer but incomplete understanding of its gating mechanism has hampered development of inhibitors for the eradication of . ...The urea channel of (UreI) is an ideal drug target for preventing gastric cancer but incomplete understanding of its gating mechanism has hampered development of inhibitors for the eradication of . Here, we present the cryo-EM structures of UreI in closed and open conformations, both at a resolution of 2.7 Å. Our hexameric structures of this small membrane protein (~21 kDa/protomer) resolve its periplasmic loops and carboxyl terminus that close and open the channel, and define a gating mechanism that is pH dependent and requires cooperativity between protomers in the hexamer. Gating is further associated with well-resolved changes in the channel-lining residues that modify the shape and length of the urea pore. Site-specific mutations in the periplasmic domain and urea pore identified key residues important for channel function. Drugs blocking the urea pore based on our structures should lead to a new strategy for eradication. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 14.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 19.9 KB 19.9 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 5.7 KB | Display | ![]() |
Images | ![]() | 3 MB | ||
Masks | ![]() | 15.6 MB | ![]() | |
Filedesc metadata | ![]() | 6.3 KB | ||
Others | ![]() ![]() | 11.1 MB 11.1 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 813.2 KB | Display | ![]() |
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Full document | ![]() | 812.7 KB | Display | |
Data in XML | ![]() | 11.4 KB | Display | |
Data in CIF | ![]() | 15.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6nskMC ![]() 0498C ![]() 6nsjC C: citing same article ( M: atomic model generated by this map |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | em-volume_P1.map | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.07 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: em-half-volume P1
File | emd_0499_half_map_1.map | ||||||||||||
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Annotation | em-half-volume_P1 | ||||||||||||
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Density Histograms |
-Half map: em-half-volume P2
File | emd_0499_half_map_2.map | ||||||||||||
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Annotation | em-half-volume_P2 | ||||||||||||
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Density Histograms |
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Sample components
-Entire : urea channel of Helicobacter pylori
Entire | Name: urea channel of Helicobacter pylori |
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Components |
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-Supramolecule #1: urea channel of Helicobacter pylori
Supramolecule | Name: urea channel of Helicobacter pylori / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 20 KDa |
-Macromolecule #1: Acid-activated urea channel
Macromolecule | Name: Acid-activated urea channel / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() Strain: J99 / ATCC 700824 |
Molecular weight | Theoretical: 22.533258 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MLGLVLLYVG IVLISNGICG LTKVDPKSTA VMNFFVGGLS IVCNVVVITY SALHPTAPVE GHHHHHHAED IVQVSHHLTS FYGPATGLL FGFTYLYAAI NHTFGLDWRP YSWYSLFVAI NTVPAAILSH YSDMLDDHKV LGITEGDWWA IIWLAWGVLW L TAFIENIL ...String: MLGLVLLYVG IVLISNGICG LTKVDPKSTA VMNFFVGGLS IVCNVVVITY SALHPTAPVE GHHHHHHAED IVQVSHHLTS FYGPATGLL FGFTYLYAAI NHTFGLDWRP YSWYSLFVAI NTVPAAILSH YSDMLDDHKV LGITEGDWWA IIWLAWGVLW L TAFIENIL KIPLGKFTPW LAIIEGILTA WIPAWLLFIQ HWV UniProtKB: Acid-activated urea channel |
-Macromolecule #2: 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHATE
Macromolecule | Name: 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHATE / type: ligand / ID: 2 / Number of copies: 18 / Formula: XP4 |
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Molecular weight | Theoretical: 591.777 Da |
Chemical component information | ![]() ChemComp-XP4: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | 2D array |
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Sample preparation
Concentration | 2 mg/mL | ||||||||
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Buffer | pH: 7 Component:
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Grid | Model: Quantifoil R2/1 / Material: COPPER / Mesh: 300 / Support film - Material: FORMVAR / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE | ||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Temperature | Min: 98.0 K / Max: 100.0 K |
Specialist optics | Energy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Dimensions - Width: 3710 pixel / Digitization - Dimensions - Height: 3838 pixel / Digitization - Frames/image: 2-59 / Number grids imaged: 1 / Number real images: 2901 / Average exposure time: 9.0 sec. / Average electron dose: 43.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 70.0 µm / Calibrated defocus max: 2.6 µm / Calibrated defocus min: 1.2 µm / Calibrated magnification: 46730 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.4 µm / Nominal defocus min: 1.4000000000000001 µm / Nominal magnification: 130000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |