|Entry||Database: EMDB / ID: EMD-0465|
|Title||Pseudomonas aeruginosa bacterial flagellar motor|
|Sample||Pseudomonas aeruginosa (bacteria)|
|Biological species||Pseudomonas aeruginosa (bacteria)|
|Method||subtomogram averaging / cryo EM / Resolution: 59 Å|
|Authors||Kaplan M / Ghosal D / Subramanian P / Oikonomou CO / Kjaer A / Pirbadian S / Ortega DR / Briegel A / El-Naggar MY / Jensen GJ|
Journal: Elife / Year: 2019
Title: The presence and absence of periplasmic rings in bacterial flagellar motors correlates with stator type.
Authors: Mohammed Kaplan / Debnath Ghosal / Poorna Subramanian / Catherine M Oikonomou / Andreas Kjaer / Sahand Pirbadian / Davi R Ortega / Ariane Briegel / Mohamed Y El-Naggar / Grant J Jensen /
Abstract: The bacterial flagellar motor, a cell-envelope-embedded macromolecular machine that functions as a cellular propeller, exhibits significant structural variability between species. Different torque- ...The bacterial flagellar motor, a cell-envelope-embedded macromolecular machine that functions as a cellular propeller, exhibits significant structural variability between species. Different torque-generating stator modules allow motors to operate in different pH, salt or viscosity levels. How such diversity evolved is unknown. Here, we use electron cryo-tomography to determine the in situ macromolecular structures of three Gammaproteobacteria motors: , , and , providing the first views of intact motors with dual stator systems. Complementing our imaging with bioinformatics analysis, we find a correlation between the motor's stator system and its structural elaboration. Motors with a single H-driven stator have only the core periplasmic P- and L-rings; those with dual H-driven stators have an elaborated P-ring; and motors with Na or Na/H-driven stators have both their P- and L-rings embellished. Our results suggest an evolution of structural elaboration that may have enabled pathogenic bacteria to colonize higher-viscosity environments in animal hosts.
|Structure viewer||EM map: |
Downloads & links
|File||Download / File: emd_0465.map.gz / Format: CCP4 / Size: 1.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)|
|Projections & slices|
Images are generated by Spider.
generated in cubic-lattice coordinate
|Voxel size||X=Y=Z: 13.06 Å|
|Symmetry||Space group: 1|
CCP4 map header:
-Entire Pseudomonas aeruginosa
|Entire||Name: Pseudomonas aeruginosa (bacteria) / Number of components: 1|
-Component #1: cellular-component, Pseudomonas aeruginosa
|Cellular-component||Name: Pseudomonas aeruginosa / Recombinant expression: No|
|Source||Species: Pseudomonas aeruginosa (bacteria)|
|Specimen||Specimen state: Cell / Method: cryo EM|
|Sample solution||pH: 7|
|Vitrification||Cryogen name: OTHER|
-Electron microscopy imaging
Model: Tecnai Polara / Image courtesy: FEI Company
|Imaging||Microscope: FEI POLARA 300|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 1 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Imaging mode: BRIGHT FIELD|
|Specimen Holder||Model: OTHER|
|Camera||Detector: GATAN K2 SUMMIT (4k x 4k)|
|Processing||Method: subtomogram averaging / Applied symmetry: C2 (2 fold cyclic) / Number of subtomograms: 144|
|3D reconstruction||Resolution: 59 Å / Resolution method: FSC 0.143 CUT-OFF|
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