+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-0338 | |||||||||
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Title | MS2 / F-pilus complex | |||||||||
Map data | ||||||||||
Sample |
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Biological species | Enterobacterio phage MS2 (virus) | |||||||||
Method | subtomogram averaging / cryo EM / Resolution: 32.3 Å | |||||||||
Authors | Hu B | |||||||||
Funding support | United States, 1 items
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Citation | Journal: Nat Commun / Year: 2019 Title: Structural basis for the adsorption of a single-stranded RNA bacteriophage. Authors: Ran Meng / Mengqiu Jiang / Zhicheng Cui / Jeng-Yih Chang / Kailu Yang / Joanita Jakana / Xinzhe Yu / Zhao Wang / Bo Hu / Junjie Zhang / Abstract: Single-stranded RNA bacteriophages (ssRNA phages) infect Gram-negative bacteria via a single maturation protein (Mat), which attaches to a retractile pilus of the host. Here we present structures of ...Single-stranded RNA bacteriophages (ssRNA phages) infect Gram-negative bacteria via a single maturation protein (Mat), which attaches to a retractile pilus of the host. Here we present structures of the ssRNA phage MS2 in complex with the Escherichia coli F-pilus, showing a network of hydrophobic and electrostatic interactions at the Mat-pilus interface. Moreover, binding of the pilus induces slight orientational variations of the Mat relative to the rest of the phage capsid, priming the Mat-connected genomic RNA (gRNA) for its release from the virions. The exposed tip of the attached Mat points opposite to the direction of the pilus retraction, which may facilitate the translocation of the gRNA from the capsid into the host cytosol. In addition, our structures determine the orientation of the assembled F-pilin subunits relative to the cell envelope, providing insights into the F-like type IV secretion systems. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_0338.map.gz | 1.9 MB | EMDB map data format | |
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Header (meta data) | emd-0338-v30.xml emd-0338.xml | 7.5 KB 7.5 KB | Display Display | EMDB header |
Images | emd_0338.png | 55.1 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-0338 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-0338 | HTTPS FTP |
-Related structure data
Related structure data | 0448C 0450C 0451C 0453C 9397C 9399C 6nm5C C: citing same article (ref.) |
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Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_0338.map.gz / Format: CCP4 / Size: 52.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Voxel size | X=Y=Z: 9 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Sample components
-Entire : MS2 / F-pilus complex
Entire | Name: MS2 / F-pilus complex |
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Components |
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-Supramolecule #1: MS2 / F-pilus complex
Supramolecule | Name: MS2 / F-pilus complex / type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: Enterobacterio phage MS2 (virus) |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | subtomogram averaging |
Aggregation state | cell |
-Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI POLARA 300 |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELDBright-field microscopy |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 1.7 e/Å2 |
Experimental equipment | Model: Tecnai Polara / Image courtesy: FEI Company |
-Image processing
Extraction | Number tomograms: 30 / Number images used: 1245 |
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Final angle assignment | Type: OTHER |
Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 32.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 1245 |