- EMDB-0204: OBP chaperonin in the nucleotide-free state -
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基本情報
登録情報
データベース: EMDB / ID: EMD-0204
タイトル
OBP chaperonin in the nucleotide-free state
マップデータ
A symmetry-free cryo-EM structure of a single-ring chaperonin encoded by gene 246 of bacteriophage OBP Pseudomonas fluorescens in the nucleotide-free state.
ジャーナル: J Struct Biol / 年: 2020 タイトル: Cryo-EM reveals an asymmetry in a novel single-ring viral chaperonin. 著者: Tatiana B Stanishneva-Konovalova / Pavel I Semenyuk / Lidia P Kurochkina / Evgeny B Pichkur / Alexander L Vasilyev / Mikhail V Kovalchuk / Mikhail P Kirpichnikov / Olga S Sokolova / 要旨: Chaperonins are ubiquitously present protein complexes, which assist the proper folding of newly synthesized proteins and prevent aggregation of denatured proteins in an ATP-dependent manner. They ...Chaperonins are ubiquitously present protein complexes, which assist the proper folding of newly synthesized proteins and prevent aggregation of denatured proteins in an ATP-dependent manner. They are classified into group I (bacterial, mitochondrial, chloroplast chaperonins) and group II (archaeal and eukaryotic cytosolic variants). However, both of these groups do not include recently discovered viral chaperonins. Here, we solved the symmetry-free cryo-EM structures of a single-ring chaperonin encoded by the gene 246 of bacteriophage OBP Pseudomonas fluorescens, in the nucleotide-free, ATPγS-, and ADP-bound states, with resolutions of 4.3 Å, 5.0 Å, and 6 Å, respectively. The structure of OBP chaperonin reveals a unique subunit arrangement, with three pairs of subunits and one unpaired subunit. Each pair combines subunits in two possible conformations, differing in nucleotide-binding affinity. The binding of nucleotides results in the increase of subunits' conformational variability. Due to its unique structural and functional features, OBP chaperonin can represent a new group.
ダウンロード / ファイル: emd_0204.map.gz / 形式: CCP4 / 大きさ: 64 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
A symmetry-free cryo-EM structure of a single-ring chaperonin encoded by gene 246 of bacteriophage OBP Pseudomonas fluorescens in the nucleotide-free state.
ボクセルのサイズ
X=Y=Z: 0.86 Å
密度
表面レベル
登録者による: 0.09 / ムービー #1: 0.09
最小 - 最大
-0.06988939 - 0.22754554
平均 (標準偏差)
0.0021357222 (±0.02195315)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
0
0
0
サイズ
256
256
256
Spacing
256
256
256
セル
A=B=C: 220.16 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
0.86
0.86
0.86
M x/y/z
256
256
256
origin x/y/z
0.000
0.000
0.000
length x/y/z
220.160
220.160
220.160
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
0
0
0
NC/NR/NS
256
256
256
D min/max/mean
-0.070
0.228
0.002
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添付データ
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追加マップ: A symmetry-free cryo-EM structure of a single-ring chaperonin...
A symmetry-free cryo-EM structure of a single-ring chaperonin encoded by gene 246 of bacteriophage OBP Pseudomonas fluorescens in the nucleotide-free state.