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- EMDB-0062: Transient state structure of CRISPR-Cpf1 (Cas12a). I2 conformation -

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Basic information

Entry
Database: EMDB / ID: EMD-0062
TitleTransient state structure of CRISPR-Cpf1 (Cas12a). I2 conformation
Map dataTransition state CRISPR-Cpf1 (Cas12a)I2 conformation
Sample
  • Complex: TRansition state complex I2 conformation
    • Complex: CRISPR-associated endonuclease Cas12a
      • Protein or peptide: CRISPR-associated endonuclease Cas12a
    • Complex: Nucleic acids
      • RNA: RNA (28-MER)
      • DNA: DNA (5'-D(P*TP*GP*AP*CP*TP*TP*CP*TP*CP*TP*AP*AP*CP*AP*AP*GP*CP*TP*CP*G)-3')
      • DNA: DNA (5'-D(P*CP*GP*AP*GP*CP*TP*CP*GP*TP*TP*AP*GP*AP*GP*AP*AP*GP*T)-3')
KeywordsCRISPR genome editing ribonucleoprotein complex / HYDROLASE
Function / homology
Function and homology information


Bacillus subtilis ribonuclease / deoxyribonuclease I / deoxyribonuclease I activity / defense response to virus / lyase activity / DNA binding / RNA binding
Similarity search - Function
: / CRISPR-associated endonuclease Cpf1 PI domain / : / CRISPR-associated endonuclease Cpf1 REC2 domain / CRISPR-associated endonuclease Cas12a / Cas12a, REC1 domain / Cas12a, RuvC nuclease domain / Cas12a, nuclease domain / Alpha helical recognition lobe domain / Nuclease domain / RuvC nuclease domain
Similarity search - Domain/homology
CRISPR-associated endonuclease Cas12a
Similarity search - Component
Biological speciesFrancisella tularensis subsp. novicida U112 (bacteria) / Francisella tularensis subsp. novicida (strain U112) (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.24 Å
AuthorsMontoya G / Mesa P
Funding support Denmark, 1 items
OrganizationGrant numberCountry
Novo Nordisk FoundationNNF14CC0001 Denmark
CitationJournal: Cell / Year: 2018
Title: Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity.
Authors: Stefano Stella / Pablo Mesa / Johannes Thomsen / Bijoya Paul / Pablo Alcón / Simon B Jensen / Bhargav Saligram / Matias E Moses / Nikos S Hatzakis / Guillermo Montoya /
Abstract: Cas12a, also known as Cpf1, is a type V-A CRISPR-Cas RNA-guided endonuclease that is used for genome editing based on its ability to generate specific dsDNA breaks. Here, we show cryo-EM structures ...Cas12a, also known as Cpf1, is a type V-A CRISPR-Cas RNA-guided endonuclease that is used for genome editing based on its ability to generate specific dsDNA breaks. Here, we show cryo-EM structures of intermediates of the cleavage reaction, thus visualizing three protein regions that sense the crRNA-DNA hybrid assembly triggering the catalytic activation of Cas12a. Single-molecule FRET provides the thermodynamics and kinetics of the conformational activation leading to phosphodiester bond hydrolysis. These findings illustrate why Cas12a cuts its target DNA and unleashes unspecific cleavage activity, degrading ssDNA molecules after activation. In addition, we show that other crRNAs are able to displace the R-loop inside the protein after target DNA cleavage, terminating indiscriminate ssDNA degradation. We propose a model whereby the conformational activation of the enzyme results in indiscriminate ssDNA cleavage. The displacement of the R-loop by a new crRNA molecule will reset Cas12a specificity, targeting new DNAs.
History
DepositionJun 18, 2018-
Header (metadata) releaseAug 22, 2018-
Map releaseDec 19, 2018-
UpdateMay 15, 2024-
Current statusMay 15, 2024Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6gtd
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_0062.png

Downloads & links

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Map

FileDownload / File: emd_0062.map.gz / Format: CCP4 / Size: 52.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationTransition state CRISPR-Cpf1 (Cas12a)I2 conformation
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.83 Å/pix.
x 240 pix.
= 199.68 Å		0.83 Å/pix.
x 240 pix.
= 199.68 Å		0.83 Å/pix.
x 240 pix.
= 199.68 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.832 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.03 / Movie #1: 0.025
Minimum - Maximum-0.09460564 - 0.18113866
Average (Standard dev.)0.00014667785 (±0.0054226513)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions240240240
Spacing240240240
CellA=B=C: 199.68001 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z0.8320.8320.832
M x/y/z240240240
origin x/y/z0.0000.0000.000
length x/y/z199.680199.680199.680
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS240240240
D min/max/mean-0.0950.1810.000

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Supplemental data

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Sample components

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Entire : TRansition state complex I2 conformation

EntireName: TRansition state complex I2 conformation
Components
  • Complex: TRansition state complex I2 conformation
    • Complex: CRISPR-associated endonuclease Cas12a
      • Protein or peptide: CRISPR-associated endonuclease Cas12a
    • Complex: Nucleic acids
      • RNA: RNA (28-MER)
      • DNA: DNA (5'-D(P*TP*GP*AP*CP*TP*TP*CP*TP*CP*TP*AP*AP*CP*AP*AP*GP*CP*TP*CP*G)-3')
      • DNA: DNA (5'-D(P*CP*GP*AP*GP*CP*TP*CP*GP*TP*TP*AP*GP*AP*GP*AP*AP*GP*T)-3')

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Supramolecule #1: TRansition state complex I2 conformation

SupramoleculeName: TRansition state complex I2 conformation / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Molecular weightTheoretical: 180 KDa

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Supramolecule #2: CRISPR-associated endonuclease Cas12a

SupramoleculeName: CRISPR-associated endonuclease Cas12a / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1
Source (natural)Organism: Francisella tularensis subsp. novicida U112 (bacteria)

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Supramolecule #3: Nucleic acids

SupramoleculeName: Nucleic acids / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2-#4
Source (natural)Organism: Francisella tularensis subsp. novicida U112 (bacteria)

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Macromolecule #1: CRISPR-associated endonuclease Cas12a

MacromoleculeName: CRISPR-associated endonuclease Cas12a / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: deoxyribonuclease I
Source (natural)Organism: Francisella tularensis subsp. novicida (strain U112) (bacteria)
Strain: U112
Molecular weightTheoretical: 155.639828 KDa
Recombinant expressionOrganism: Escherichia coli BL21 (bacteria)
SequenceString: MSIYQEFVNK YSLSKTLRFE LIPQGKTLEN IKARGLILDD EKRAKDYKKA KQIIDKYHQF FIEEILSSVC ISEDLLQNYS DVYFKLKKS DDDNLQKDFK SAKDTIKKQI SEYIKDSEKF KNLFNQNLID AKKGQESDLI LWLKQSKDNG IELFKANSDI T DIDEALEI ...String:
MSIYQEFVNK YSLSKTLRFE LIPQGKTLEN IKARGLILDD EKRAKDYKKA KQIIDKYHQF FIEEILSSVC ISEDLLQNYS DVYFKLKKS DDDNLQKDFK SAKDTIKKQI SEYIKDSEKF KNLFNQNLID AKKGQESDLI LWLKQSKDNG IELFKANSDI T DIDEALEI IKSFKGWTTY FKGFHENRKN VYSSNDIPTS IIYRIVDDNL PKFLENKAKY ESLKDKAPEA INYEQIKKDL AE ELTFDID YKTSEVNQRV FSLDEVFEIA NFNNYLNQSG ITKFNTIIGG KFVNGENTKR KGINEYINLY SQQINDKTLK KYK MSVLFK QILSDTESKS FVIDKLEDDS DVVTTMQSFY EQIAAFKTVE EKSIKETLSL LFDDLKAQKL DLSKIYFKND KSLT DLSQQ VFDDYSVIGT AVLEYITQQI APKNLDNPSK KEQELIAKKT EKAKYLSLET IKLALEEFNK HRDIDKQCRF EEILA NFAA IPMIFDEIAQ NKDNLAQISI KYQNQGKKDL LQASAEDDVK AIKDLLDQTN NLLHKLKIFH ISQSEDKANI LDKDEH FYL VFEECYFELA NIVPLYNKIR NYITQKPYSD EKFKLNFENS TLANGWDKNK EPDNTAILFI KDDKYYLGVM NKKNNKI FD DKAIKENKGE GYKKIVYKLL PGANKMLPKV FFSAKSIKFY NPSEDILRIR NHSTHTKNGS PQKGYEKFEF NIEDCRKF I DFYKQSISKH PEWKDFGFRF SDTQRYNSID EFYREVENQG YKLTFENISE SYIDSVVNQG KLYLFQIYNK DFSAYSKGR PNLHTLYWKA LFDERNLQDV VYKLNGEAEL FYRKQSIPKK ITHPAKEAIA NKNKDNPKKE SVFEYDLIKD KRFTEDKFFF HCPITINFK SSGANKFNDE INLLLKEKAN DVHILSIDRG ERHLAYYTLV DGKGNIIKQD TFNIIGNDRM KTNYHDKLAA I EKDRDSAR KDWKKINNIK EMKEGYLSQV VHEIAKLVIE YNAIVVFQDL NFGFKRGRFK VEKQVYQKLE KMLIEKLNYL VF KDNEFDK TGGVLRAYQL TAPFETFKKM GKQTGIIYYV PAGFTSKICP VTGFVNQLYP KYESVSKSQE FFSKFDKICY NLD KGYFEF SFDYKNFGDK AAKGKWTIAS FGSRLINFRN SDKNHNWDTR EVYPTKELEK LLKDYSIEYG HGECIKAAIC GESD KKFFA KLTSVLNTIL QMRNSKTGTE LDYLISPVAD VNGNFFDSRQ APKNMPQDAD ANGAYHIGLK GLMLLGRIKN NQEGK KLNL VIKNEEYFEF VQNRNNGSEF ELENLYFQGE LRRQASALEH HHHHH

UniProtKB: CRISPR-associated endonuclease Cas12a

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Macromolecule #2: RNA (28-MER)

MacromoleculeName: RNA (28-MER) / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Francisella tularensis subsp. novicida U112 (bacteria)
Molecular weightTheoretical: 13.749146 KDa
SequenceString:
AAUUUCUACU GUUGUAGAUG AGAAGUCAUU UAAUAAGGCC ACU

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Macromolecule #3: DNA (5'-D(P*TP*GP*AP*CP*TP*TP*CP*TP*CP*TP*AP*AP*CP*AP*AP*GP*CP*TP...

MacromoleculeName: DNA (5'-D(P*TP*GP*AP*CP*TP*TP*CP*TP*CP*TP*AP*AP*CP*AP*AP*GP*CP*TP*CP*G)-3')
type: dna / ID: 3 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Francisella tularensis subsp. novicida U112 (bacteria)
Molecular weightTheoretical: 16.898826 KDa
SequenceString: (DA)(DT)(DT)(DG)(DC)(DT)(DT)(DG)(DC)(DT) (DC)(DG)(DA)(DT)(DG)(DC)(DA)(DT)(DG)(DC) (DA)(DG)(DT)(DG)(DG)(DC)(DC)(DT)(DT) (DA)(DT)(DT)(DA)(DA)(DA)(DT)(DG)(DA)(DC) (DT) (DT)(DC)(DT)(DC)(DT)(DA) ...String:
(DA)(DT)(DT)(DG)(DC)(DT)(DT)(DG)(DC)(DT) (DC)(DG)(DA)(DT)(DG)(DC)(DA)(DT)(DG)(DC) (DA)(DG)(DT)(DG)(DG)(DC)(DC)(DT)(DT) (DA)(DT)(DT)(DA)(DA)(DA)(DT)(DG)(DA)(DC) (DT) (DT)(DC)(DT)(DC)(DT)(DA)(DA)(DC) (DG)(DA)(DG)(DC)(DT)(DC)(DG)

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Macromolecule #4: DNA (5'-D(P*CP*GP*AP*GP*CP*TP*CP*GP*TP*TP*AP*GP*AP*GP*AP*AP*GP*T)-3')

MacromoleculeName: DNA (5'-D(P*CP*GP*AP*GP*CP*TP*CP*GP*TP*TP*AP*GP*AP*GP*AP*AP*GP*T)-3')
type: dna / ID: 4 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Francisella tularensis subsp. novicida U112 (bacteria)
Molecular weightTheoretical: 16.992936 KDa
SequenceString: (DC)(DG)(DA)(DG)(DC)(DT)(DC)(DG)(DT)(DT) (DA)(DG)(DA)(DG)(DA)(DA)(DG)(DT)(DC)(DA) (DT)(DT)(DT)(DA)(DA)(DT)(DA)(DA)(DG) (DG)(DC)(DC)(DA)(DC)(DT)(DG)(DC)(DA)(DT) (DG) (DC)(DA)(DT)(DC)(DG)(DA) ...String:
(DC)(DG)(DA)(DG)(DC)(DT)(DC)(DG)(DT)(DT) (DA)(DG)(DA)(DG)(DA)(DA)(DG)(DT)(DC)(DA) (DT)(DT)(DT)(DA)(DA)(DT)(DA)(DA)(DG) (DG)(DC)(DC)(DA)(DC)(DT)(DG)(DC)(DA)(DT) (DG) (DC)(DA)(DT)(DC)(DG)(DA)(DG)(DC) (DA)(DA)(DG)(DC)(DA)(DA)(DT)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7
GridModel: C-flat-1.2/1.3 4C / Material: COPPER
VitrificationCryogen name: ETHANE / Instrument: FEI VITROBOT MARK III

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: COUNTING / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.24 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 124000
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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