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- SASDGL2: Ring opening PaaZ from the phenylacetate degradation pathway (E. ... -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDGL2
SampleRing opening PaaZ from the phenylacetate degradation pathway (E. coli K12)
  • Bifunctional protein PaaZ (protein), PaaZ, Escherichia coli (strain K12)
Function / homology
Function and homology information


3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase / oxepin-CoA hydrolase / hydrolase activity, acting on acid carbon-carbon bonds, in ketonic substances / ether hydrolase activity / oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor / phenylacetate catabolic process / oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor / enoyl-CoA hydratase activity / identical protein binding
Similarity search - Function
Phenylacetic acid degradation protein PaaN / MaoC-like dehydratase domain / MaoC like domain / HotDog domain superfamily / Aldehyde dehydrogenase domain / Aldehyde dehydrogenase family / Aldehyde dehydrogenase, N-terminal / Aldehyde dehydrogenase, C-terminal / Aldehyde/histidinol dehydrogenase
Similarity search - Domain/homology
Bifunctional protein PaaZ
Similarity search - Component
Biological speciesEscherichia coli (strain K12) (bacteria)
Contact author
  • Lokesh Gakhar (University of Iowa, Iowa, USA)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #3569
Type: atomic / Chi-square value: 3.382
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Ring opening PaaZ from the phenylacetate degradation pathway (E. coli K12)
Specimen concentration: 1.50-4.50
BufferName: 25 mM HEPES, 50 mM NaCl / pH: 7.4
Entity #1796Name: PaaZ / Type: protein / Description: Bifunctional protein PaaZ / Formula weight: 73.002 / Num. of mol.: 6 / Source: Escherichia coli (strain K12) / References: UniProt: P77455
Sequence: MQQLASFLSG TWQSGRGRSR LIHHAISGEA LWEVTSEGLD MAAARQFAIE KGAPALRAMT FIERAAMLKA VAKHLLSEKE RFYALSAQTG ATRADSWVDI EGGIGTLFTY ASLGSRELPD DTLWPEDELI PLSKEGGFAA RHLLTSKSGV AVHINAFNFP CWGMLEKLAP ...Sequence:
MQQLASFLSG TWQSGRGRSR LIHHAISGEA LWEVTSEGLD MAAARQFAIE KGAPALRAMT FIERAAMLKA VAKHLLSEKE RFYALSAQTG ATRADSWVDI EGGIGTLFTY ASLGSRELPD DTLWPEDELI PLSKEGGFAA RHLLTSKSGV AVHINAFNFP CWGMLEKLAP TWLGGMPAII KPATATAQLT QAMVKSIVDS GLVPEGAISL ICGSAGDLLD HLDSQDVVTF TGSAATGQML RVQPNIVAKS IPFTMEADSL NCCVLGEDVT PDQPEFALFI REVVREMTTK AGQKCTAIRR IIVPQALVNA VSDALVARLQ KVVVGDPAQE GVKMGALVNA EQRADVQEKV NILLAAGCEI RLGGQADLSA AGAFFPPTLL YCPQPDETPA VHATEAFGPV ATLMPAQNQR HALQLACAGG GSLAGTLVTA DPQIARQFIA DAARTHGRIQ ILNEESAKES TGHGSPLPQL VHGGPGRAGG GEELGGLRAV KHYMQRTAVQ GSPTMLAAIS KQWVRGAKVE EDRIHPFRKY FEELQPGDSL LTPRRTMTEA DIVNFACLSG DHFYAHMDKI AAAESIFGER VVHGYFVLSA AAGLFVDAGV GPVIANYGLE SLRFIEPVKP GDTIQVRLTC KRKTLKKQRS AEEKPTGVVE WAVEVFNQHQ TPVALYSILT LVARQHGDFV D

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Experimental information

BeamInstrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS)
City: Berkeley, CA / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.103 Å / Dist. spec. to detc.: 1.5 mm
DetectorName: MAR 165 CCD
Scan
Title: Ring opening PaaZ from the phenylacetate degradation pathway (E. coli K12)
Measurement date: Feb 24, 2015 / Storage temperature: 4 °C / Cell temperature: 10 °C / Exposure time: 5 sec. / Number of frames: 1 / Unit: 1/A /
MinMax
Q0.0119 0.3189
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 207 /
MinMax
Q0.013683 0.138954
P(R) point1 207
R0 200.2
Result
Type of curve: merged /
ExperimentalPorod
MW433.6 kDa400 kDa
Volume-636 nm3

P(R)GuinierGuinier error
Forward scattering, I06450 6483.9 -
Radius of gyration, Rg6.21 nm6.2 nm0.1

MinMax
D-20.02
Guinier point4 16

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