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- SASDGF2: Putative mitochondrial transferase CAF17 bound to mitochondrial i... -

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Basic information

Entry
Database: SASBDB / ID: SASDGF2
SamplePutative mitochondrial transferase CAF17 bound to mitochondrial iron-sulfur cluster assembly ISCA2 protein (human IBA57-ISCA2 complex)
  • Putative transferase CAF17, mitochondrial (protein), IBA57, Homo sapiens
  • Iron-sulfur cluster assembly 2 homolog, mitochondrial (protein), ISCA2, Homo sapiens
Function / homology
Function and homology information


mitochondrial [4Fe-4S] assembly complex / Transferases; Transferring one-carbon groups / protein maturation by [4Fe-4S] cluster transfer / Mitochondrial iron-sulfur cluster biogenesis / Maturation of TCA enzymes and regulation of TCA cycle / heme biosynthetic process / iron-sulfur cluster assembly / 2 iron, 2 sulfur cluster binding / 4 iron, 4 sulfur cluster binding / transferase activity ...mitochondrial [4Fe-4S] assembly complex / Transferases; Transferring one-carbon groups / protein maturation by [4Fe-4S] cluster transfer / Mitochondrial iron-sulfur cluster biogenesis / Maturation of TCA enzymes and regulation of TCA cycle / heme biosynthetic process / iron-sulfur cluster assembly / 2 iron, 2 sulfur cluster binding / 4 iron, 4 sulfur cluster binding / transferase activity / mitochondrial matrix / iron ion binding / mitochondrion / RNA binding / identical protein binding
Similarity search - Function
YgfZ/GcvT conserved site / FeS cluster insertion, C-terminal, conserved site / YgfZ/GcvT / Hypothetical hesB/yadR/yfhF family signature. / FeS cluster insertion protein / FeS cluster biogenesis / HesB-like domain superfamily / Iron-sulphur cluster biosynthesis / GTP-binding protein TrmE/Aminomethyltransferase GcvT, domain 1
Similarity search - Domain/homology
Putative transferase CAF17, mitochondrial / Iron-sulfur cluster assembly 2 homolog, mitochondrial
Similarity search - Component
Biological speciesHomo sapiens (human)
Contact author
  • Stefano Da Vela (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #3577
Type: atomic / Radius of dummy atoms: 1.90 A / Chi-square value: 1.365
Search similar-shape structures of this assembly by Omokage search (details)
Model #3578
Type: dummy / Radius of dummy atoms: 1.90 A
Comment: Monsa model (together with the ISCA2, see entry SASDGE2)
Chi-square value: 1.197
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Putative mitochondrial transferase CAF17 bound to mitochondrial iron-sulfur cluster assembly ISCA2 protein (human IBA57-ISCA2 complex)
Specimen concentration: 0.9 mg/ml / Entity id: 1801 / 1802
BufferName: 50 mM phosphate, 150 mM NaCl, 5 mM DTT / pH: 7
Entity #1801Name: IBA57 / Type: protein / Description: Putative transferase CAF17, mitochondrial / Formula weight: 33.242 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q5T440
Sequence: AGAAWACFRL DGRTLLRVRG PDAAPFLLGL LTNELPLPSP AAAGAPPAAR AGYAHFLNVQ GRTLYDVILY GLQEHSEVSG FLLECDSSVQ GALQKHLALY RIRRKVTVEP HPELRVWAVL PSSPEACGAA SLQERAGAAA ILIRDPRTAR MGWRLLTQDE GPALVPGGRL ...Sequence:
AGAAWACFRL DGRTLLRVRG PDAAPFLLGL LTNELPLPSP AAAGAPPAAR AGYAHFLNVQ GRTLYDVILY GLQEHSEVSG FLLECDSSVQ GALQKHLALY RIRRKVTVEP HPELRVWAVL PSSPEACGAA SLQERAGAAA ILIRDPRTAR MGWRLLTQDE GPALVPGGRL GDLWDYHQHR YLQGVPEGVR DLPPGVALPL ESNLAFMNGV SFTKGCYIGQ ELTARTHHMG VIRKRLFPVR FLDPLPTSGI TPGATVLTAS GQTVGKFRAG QGNVGLALLW SEKIKGPLHI RASEGAQVAL AASVPDWWP
Entity #1802Name: ISCA2 / Type: protein
Description: Iron-sulfur cluster assembly 2 homolog, mitochondrial
Formula weight: 12.041 / Num. of mol.: 2 / Source: Homo sapiens / References: UniProt: Q86U28
Sequence:
EAGEGQIRLT DSCVQRLLEI TEGSEFLRLQ VEGGGCSGFQ YKFSLDTVIN PDDRVFEQGG ARVVVDSDSL AFVKGAQVDF SQELIRSSFQ VLNNPQAQQG CSCGSSFSIK L

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3 mm
DetectorName: Pilatus 6M
Scan
Title: Putative mitochondrial transferase CAF17 bound to mitochondrial iron-sulfur cluster assembly ISCA2 protein (human IBA57-ISCA2 complex)
Measurement date: Nov 22, 2018 / Storage temperature: 10 °C / Cell temperature: 20 °C / Exposure time: 0.095 sec. / Number of frames: 40 / Unit: 1/nm /
MinMax
Q0.0859 2.9981
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 902 /
MinMax
Q0.0941803 2.5742
P(R) point1 902
R0 16.38
Result
Type of curve: single_conc
ExperimentalStandardPorod
MW76.35 kDa79.9 kDa71 kDa
Volume--114 nm3

P(R)GuinierGuinier error
Forward scattering, I00.06335 0.06197 0.00025
Radius of gyration, Rg4.313 nm3.95 nm0.12

MinMax
D-16.38
Guinier point4 89

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