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- SASDG94: Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino a... -

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Basic information

Entry
Database: SASBDB / ID: SASDG94
SampleAutoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (antiparallel CORAL and MultiFoXS models)
  • Cytohesin-3 (protein), Cyth3, Grp1, Mus musculus
Function / homology
Function and homology information


Intra-Golgi traffic / Golgi vesicle transport / establishment of epithelial cell polarity / regulation of ARF protein signal transduction / phosphatidylinositol-3,4,5-trisphosphate binding / bicellular tight junction / ruffle / positive regulation of cell adhesion / guanyl-nucleotide exchange factor activity / adherens junction ...Intra-Golgi traffic / Golgi vesicle transport / establishment of epithelial cell polarity / regulation of ARF protein signal transduction / phosphatidylinositol-3,4,5-trisphosphate binding / bicellular tight junction / ruffle / positive regulation of cell adhesion / guanyl-nucleotide exchange factor activity / adherens junction / nucleoplasm / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Sec7 domain / Sec7, C-terminal domain superfamily / Sec7 domain superfamily / Sec7 domain / SEC7 domain profile. / Sec7 domain / PH domain / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain / PH-like domain superfamily
Similarity search - Domain/homology
Biological speciesMus musculus (house mouse)
Contact author
  • David Lambright (UMASS, UMASS Medical School, Worcester, MA, USA)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #3655
Type: mix / Software: (CORAL05) / Radius of dummy atoms: 1.90 A / Symmetry: P1
Comment: Derived from Grp1 63-399 (2R09) and a canonical antiparallel coiled coil
Chi-square value: 2.84
Search similar-shape structures of this assembly by Omokage search (details)
Model #3656
Type: atomic / Symmetry: P1
Comment: Derived from Grp1 63-399 (2R09) and a canonical antiparallel coiled coil
Chi-square value: 0.859877586721 / P-value: 0.064794
Search similar-shape structures of this assembly by Omokage search (details)
Model #3657
Type: atomic / Symmetry: P1
Comment: Derived from Grp1 63-399 (2R09) and a canonical antiparallel coiled coil
Chi-square value: 0.859877586721 / P-value: 0.064794
Search similar-shape structures of this assembly by Omokage search (details)
Model #3658
Type: atomic / Symmetry: P1
Comment: Derived from Grp1 63-399 (2R09) and a canonical antiparallel coiled coil
Chi-square value: 0.859877586721 / P-value: 0.064794
Search similar-shape structures of this assembly by Omokage search (details)
Model #3659
Type: atomic / Symmetry: P1
Comment: Derived from Grp1 63-399 (2R09) and a canonical antiparallel coiled coil
Chi-square value: 0.859877586721 / P-value: 0.064794
Search similar-shape structures of this assembly by Omokage search (details)
Model #3660
Type: atomic / Symmetry: P1
Comment: Derived from Grp1 63-399 (2R09) and a canonical antiparallel coiled coil
Chi-square value: 0.859877586721 / P-value: 0.064794
Search similar-shape structures of this assembly by Omokage search (details)
Model #3661
Type: atomic / Symmetry: P1
Comment: Derived from Grp1 63-399 (2R09) and a canonical antiparallel coiled coil
Chi-square value: 0.859877586721 / P-value: 0.064794
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (antiparallel CORAL and MultiFoXS models)
Specimen concentration: 12 mg/ml
BufferName: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate
pH: 8
Entity #1853Name: Cyth3, Grp1 / Type: protein / Description: Cytohesin-3 / Formula weight: 46.304 / Num. of mol.: 2 / Source: Mus musculus / References: UniProt: O08967
Sequence: MGHHHHHHGS EDLSLEEREE LLDIRRRKKE LIDDIERLKY EIAEVMTEID NLTSVEESKT TQRNKQIAMG RKKFNMDPKK GIQFLIENDL LQSSPEDVAQ FLYKGEGLNK TVIGDYLGER DDFNIKVLQA FVELHEFADL NLVQALRQFL WSFRLPGEAQ KIDRMMEAFA ...Sequence:
MGHHHHHHGS EDLSLEEREE LLDIRRRKKE LIDDIERLKY EIAEVMTEID NLTSVEESKT TQRNKQIAMG RKKFNMDPKK GIQFLIENDL LQSSPEDVAQ FLYKGEGLNK TVIGDYLGER DDFNIKVLQA FVELHEFADL NLVQALRQFL WSFRLPGEAQ KIDRMMEAFA SRYCLCNPGV FQSTDTCYVL SFAIIMLNTS LHNHNVRDKP TAERFITMNR GINEGGDLPE ELLRNLYESI KNEPFKIPED DGNDLTHTFF NPDREGWLLK LGGRVKTWKR RWFILTDNCL YYFEYTTDKE PRGIIPLENL SIREVEDPRK PNCFELYNPS HKGQVIKACK TEADGRVVEG NHVVYRISAP SPEEKEEWMK SIKASISRDP FYDMLATRKR RIANKK

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Experimental information

BeamInstrument name: Advanced Photon Source (APS), Argonne National Laboratory BioCAT 18ID
City: Lemont, IL / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.10332 Å / Dist. spec. to detc.: 2.5 mm
DetectorName: MAR 165 CCD
Scan
Title: Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (antiparallel CORAL and MultiFoXS models)
Measurement date: Nov 15, 2013 / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 132 / Unit: 1/A /
MinMax
Q0.0069 0.3162
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 76 /
MinMax
Q0.00688375 0.316224
P(R) point1 76
R0 260
Result
Type of curve: sec
Comments: The protein was equilibrated with a 1.2 molar excess of inositol 1,3,4,5-tetrakis phosphate (IP4) and concentrated to 12 mg/ml, prior to injection.
ExperimentalPorod
MW93.6 kDa-
Volume-194 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I01.062 0.002 1.05 0.001
Radius of gyration, Rg5.73 nm0.06 5.46 nm0.02

MinMaxError
D-26 0.1
Guinier point1 20 -

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