SASDFE2: wild-type human Latency Associated Peptide (LAP) (Latency Associa...

基本情報

登録情報

データベース: SASBDB / ID: SASDFE2

試料

wild-type human Latency Associated Peptide (LAP)

• Latency Associated Peptide (protein), LAP, Homo sapiens

機能・相同性

分子機能:

cellular response to acetaldehyde / frontal suture morphogenesis / Influenza Virus Induced Apoptosis / adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains / positive regulation of microglia differentiation / regulation of interleukin-23 production / branch elongation involved in mammary gland duct branching / positive regulation of primary miRNA processing / columnar/cuboidal epithelial cell maturation / negative regulation of skeletal muscle tissue development / macrophage derived foam cell differentiation / response to laminar fluid shear stress / embryonic liver development / regulation of enamel mineralization / regulation of branching involved in mammary gland duct morphogenesis / regulation of cartilage development / TGFBR2 MSI Frameshift Mutants in Cancer / regulation of striated muscle tissue development / regulation of blood vessel remodeling / regulation of protein import into nucleus / regulatory T cell differentiation / tolerance induction to self antigen / extracellular matrix assembly / negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target / negative regulation of hyaluronan biosynthetic process / type III transforming growth factor beta receptor binding / myofibroblast differentiation / positive regulation of cardiac muscle cell differentiation / positive regulation of odontogenesis / connective tissue replacement involved in inflammatory response wound healing / Langerhans cell differentiation / TGFBR2 Kinase Domain Mutants in Cancer / positive regulation of smooth muscle cell differentiation / positive regulation of exit from mitosis / negative regulation of macrophage cytokine production / odontoblast differentiation / SMAD2/3 Phosphorylation Motif Mutants in Cancer / TGFBR1 KD Mutants in Cancer / positive regulation of isotype switching to IgA isotypes / positive regulation of mesenchymal stem cell proliferation / positive regulation of receptor signaling pathway via STAT / membrane protein intracellular domain proteolysis / positive regulation of extracellular matrix assembly / retina vasculature development in camera-type eye / heart valve morphogenesis / bronchiole development / mammary gland branching involved in thelarche / hyaluronan catabolic process / TGFBR3 regulates TGF-beta signaling / positive regulation of vasculature development / lens fiber cell differentiation / ATP biosynthetic process / negative regulation of extracellular matrix disassembly / type II transforming growth factor beta receptor binding / receptor catabolic process / positive regulation of branching involved in ureteric bud morphogenesis / TGFBR1 LBD Mutants in Cancer / positive regulation of chemotaxis / type I transforming growth factor beta receptor binding / response to salt / germ cell migration / positive regulation of mononuclear cell migration / endoderm development / negative regulation of biomineral tissue development / phospholipid homeostasis / negative regulation of cell-cell adhesion mediated by cadherin / negative regulation of myoblast differentiation / positive regulation of vascular permeability / response to cholesterol / oligodendrocyte development / cell-cell junction organization / negative regulation of interleukin-17 production / phosphate-containing compound metabolic process / surfactant homeostasis / deubiquitinase activator activity / sprouting angiogenesis / negative regulation of release of sequestered calcium ion into cytosol / positive regulation of chemokine (C-X-C motif) ligand 2 production / negative regulation of ossification / digestive tract development / aortic valve morphogenesis / RUNX3 regulates CDKN1A transcription / response to vitamin D / face morphogenesis / positive regulation of fibroblast migration / ureteric bud development / neural tube development / positive regulation of regulatory T cell differentiation / positive regulation of peptidyl-tyrosine phosphorylation / Molecules associated with elastic fibres / negative regulation of phagocytosis / positive regulation of epidermal growth factor receptor signaling pathway / negative regulation of neuroblast proliferation / lung alveolus development / Syndecan interactions / ventricular cardiac muscle tissue morphogenesis / muscle cell cellular homeostasis / cellular response to insulin-like growth factor stimulus / negative regulation of fat cell differentiation / odontogenesis of dentin-containing tooth

ドメイン・相同性:

Transforming growth factor beta-1 proprotein / Transforming growth factor-beta / TGF-beta, propeptide / TGF-beta propeptide / Transforming growth factor beta, conserved site / TGF-beta family signature. / Transforming growth factor-beta-related / Transforming growth factor-beta (TGF-beta) family / Transforming growth factor-beta, C-terminal / Transforming growth factor beta like domain / TGF-beta family profile. / Cystine-knot cytokine

構成要素:

Transforming growth factor beta-1 proprotein

• 生物種: Homo sapiens (ヒト)
• 引用: 日付: 2019 Jul 1; タイトル: Structural consequences of transforming growth factor beta-1 activation from near-therapeutic X-ray doses; 著者: Stachowski T / Grant T

登録者

• Tim Stachowski (Hauptman-Woodward Medical Research Institute, New York, USA)

モデル

試料

• 試料: 名称: wild-type human Latency Associated Peptide (LAP) / 試料濃度: 4.5 mg/ml
• バッファ: 名称: phosphate buffered saline / pH: 7.4

要素 #1479

名称: LAP / タイプ: protein / 記述: Latency Associated Peptide / 分子量: 29.212 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: P01137
配列:

LSTSKTIDME LVKRKRIEAI RGQILSKLRL ASPPSQGEVP PGPLPEAVLA LYNSTRDRVA GESAEPEPEP EADYYAKEVT RVLMVETHNE IYDKFKQSTH SIYMFFNTSE LREAVPEPVL LSRAELRLLR LKLKVEQHVE LYQKYSNNSW RYLSNRLLAP SDSPEWLSFD VTGVVRQWLS RGGEIEGFRL SAHCSCDSRD NTLQVDINGF TTGRRGDLAT IHGMNRPFLL LMATPLERAQ HLQSSRHRAH HHHHH

実験情報

• ビーム: 設備名称: Advanced Light Source (ALS) 12.3.1 (SIBYLS) / 地域: Berkeley, CA / 国: USA / 線源: X-ray synchrotron / 波長: 0.1127 Å / スペクトロメータ・検出器間距離: 2 mm
• 検出器: 名称: Pilatus3 X 2M / Pixsize x: 172 mm

スキャン

タイトル: wild-type human Latency Associated Peptide (LAP)
測定日: 2018年10月4日 / 保管温度: 4 °C / セル温度: 10 °C / 照射時間: 0.1 sec. / フレーム数: 4 / 単位: 1/A /

	Min	Max
Q	0.0114	0.4038

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 702 /

	Min	Max
Q	0.011428	0.402113
P(R) point	1	702
R	0	175

結果

カーブのタイプ: single_conc /

	実験値	Porod
分子量	91 kDa	110 kDa
体積	-	179 nm3

	P(R)	Guinier	Guinier error
前方散乱 I0	28.63	28.17	0.077
慣性半径, Rg	4.36 nm	4.1 nm	0.018

	Min	Max
D	-	17.5
Guinier point	1	34