+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDF54 |
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Sample | RXRΔH12/RAR Heterodimer : N-CoRNID Complex (1:1)
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Function / homology | Function and homology information Transcriptional regulation of granulopoiesis / Carnitine metabolism / Transcriptional regulation of white adipocyte differentiation / Signaling by Retinoic Acid / NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis / definitive erythrocyte differentiation / NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux / CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / SUMOylation of intracellular receptors / Downregulation of SMAD2/3:SMAD4 transcriptional activity ...Transcriptional regulation of granulopoiesis / Carnitine metabolism / Transcriptional regulation of white adipocyte differentiation / Signaling by Retinoic Acid / NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis / definitive erythrocyte differentiation / NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux / CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / SUMOylation of intracellular receptors / Downregulation of SMAD2/3:SMAD4 transcriptional activity / Recycling of bile acids and salts / Synthesis of bile acids and bile salts / Nuclear Receptor transcription pathway / ventricular cardiac muscle cell differentiation / visceral serous pericardium development / Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol / Synthesis of bile acids and bile salts via 27-hydroxycholesterol / HDACs deacetylate histones / mesenchyme development / positive regulation of translational initiation by iron / Endogenous sterols / Notch-HLH transcription pathway / maternal placenta development / Regulation of lipid metabolism by PPARalpha / thalamus development / secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development / retinoic acid-responsive element binding / Cytoprotection by HMOX1 / positive regulation of thyroid hormone receptor signaling pathway / angiogenesis involved in coronary vascular morphogenesis / cardiac muscle cell differentiation / nuclear retinoic acid receptor binding / camera-type eye development / regulation of thyroid hormone receptor signaling pathway / peroxisome proliferator activated receptor binding / positive regulation of vitamin D receptor signaling pathway / histone deacetylase regulator activity / nuclear thyroid hormone receptor binding / cardiac muscle cell proliferation / ventricular cardiac muscle tissue morphogenesis / regulation of myelination / nuclear steroid receptor activity / RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding / regulation of branching involved in prostate gland morphogenesis / locomotor rhythm / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / cellular response to Thyroglobulin triiodothyronine / regulation of multicellular organism growth / epidermis development / retinoic acid receptor signaling pathway / establishment of skin barrier / positive regulation of bone mineralization / nuclear retinoid X receptor binding / heart morphogenesis / response to retinoic acid / response to glucocorticoid / transcription repressor complex / embryo implantation / hormone-mediated signaling pathway / cholesterol homeostasis / nuclear estrogen receptor binding / RNA polymerase II transcription regulatory region sequence-specific DNA binding / circadian regulation of gene expression / mRNA transcription by RNA polymerase II / placenta development / chromatin DNA binding / transcription coactivator binding / histone deacetylase binding / RNA polymerase II transcription regulator complex / transcription corepressor activity / nuclear receptor activity / chromatin organization / heart development / gene expression / T cell differentiation in thymus / in utero embryonic development / transcription regulator complex / sequence-specific DNA binding / cell differentiation / protein stabilization / transcription cis-regulatory region binding / positive regulation of apoptotic process / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / protein domain specific binding / negative regulation of cell population proliferation / axon / negative regulation of gene expression / negative regulation of DNA-templated transcription / chromatin binding / positive regulation of cell population proliferation / regulation of DNA-templated transcription / protein-containing complex binding / chromatin / regulation of transcription by RNA polymerase II / perinuclear region of cytoplasm / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / mitochondrion Similarity search - Function |
Biological species | Mouse (Mus musculus) |
Citation | Journal: Structure / Year: 2019 Title: Interplay of Protein Disorder in Retinoic Acid Receptor Heterodimer and Its Corepressor Regulates Gene Expression. Authors: Tiago N Cordeiro / Nathalie Sibille / Pierre Germain / Philippe Barthe / Abdelhay Boulahtouf / Fréderic Allemand / Rémy Bailly / Valérie Vivat / Christine Ebel / Alessandro Barducci / ...Authors: Tiago N Cordeiro / Nathalie Sibille / Pierre Germain / Philippe Barthe / Abdelhay Boulahtouf / Fréderic Allemand / Rémy Bailly / Valérie Vivat / Christine Ebel / Alessandro Barducci / William Bourguet / Albane le Maire / Pau Bernadó / Abstract: In its unliganded form, the retinoic acid receptor (RAR) in heterodimer with the retinoid X receptor (RXR) exerts a strong repressive activity facilitated by the recruitment of transcriptional ...In its unliganded form, the retinoic acid receptor (RAR) in heterodimer with the retinoid X receptor (RXR) exerts a strong repressive activity facilitated by the recruitment of transcriptional corepressors in the promoter region of target genes. By integrating complementary structural, biophysical, and computational information, we demonstrate that intrinsic disorder is a required feature for the precise regulation of RAR activity. We show that structural dynamics of RAR and RXR H12 regions is an essential mechanism for RAR regulation. Unexpectedly we found that, while mainly disordered, the corepressor N-CoR presents evolutionary conserved structured regions involved in transient intramolecular contacts. In the presence of RXR/RAR, N-CoR exploits its multivalency to form a cooperative multisite complex that displays equilibrium between different conformational states that can be tuned by cognate ligands and receptor mutations. This equilibrium is key to preserving the repressive basal state while allowing the conversion to a transcriptionally active form. |
Contact author |
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-Structure visualization
-Downloads & links
-Data source
SASBDB page | SASDF54 |
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-Related structure data
Related structure data | C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-External links
Related items in Molecule of the Month |
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-Models
-Sample
Sample | Name: RXRΔH12/RAR Heterodimer : N-CoRNID Complex (1:1) / Specimen concentration: 0.80-11.20 / Entity id: 1515 / 1536 / 1538 |
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Buffer | Name: 50mM Tris HCl, 150mM NaCl, 2mM TCEP. / pH: 7.5 |
Entity #1515 | Name: N-CoR-NID / Type: protein Description: Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) Formula weight: 29.139 / Num. of mol.: 1 / Source: Mouse (Mus musculus) / References: UniProt: Q60974 Sequence: GPHMQVPRTH RLITLADHIC QIITQDFARN QVPSQASTST FQTSPSALSS TPVRTKTSSR YSPESQSQTV LHPRPGPRVS PENLVDKSRG SRPGKSPERS HIPSEPYEPI SPPQGPAVHE KQDSMLLLSQ RGVDPAEQRS DSRSPGSISY LPSFFTKLES TSPMVKSKKQ ...Sequence: GPHMQVPRTH RLITLADHIC QIITQDFARN QVPSQASTST FQTSPSALSS TPVRTKTSSR YSPESQSQTV LHPRPGPRVS PENLVDKSRG SRPGKSPERS HIPSEPYEPI SPPQGPAVHE KQDSMLLLSQ RGVDPAEQRS DSRSPGSISY LPSFFTKLES TSPMVKSKKQ EIFRKLNSSG GGDSDMAAAQ PGTEIFNLPA VTTSGAVSSR SHSFADPASN LGLEDIIRKA LMGSFDDKVE DHGVVMSHPV GIMPGSASTS VVTSSEARRD E |
Entity #1536 | Name: RXR / Type: protein Description: Retinoid-X receptor alpha (RXR-alpha) Ligand Binding Domain (LBD) Formula weight: 26.47 / Num. of mol.: 1 / Source: Mouse (Mus musculus) / References: UniProt: P28700 Sequence: SANEDMPVEK ILEAELAVEP KTETYVEANM GLNPSSPNDP VTNICQAADK QLFTLVEWAK RIPHFSELPL DDQVILLRAG WNELLIASAS HRSIAVKDGI LLATGLHVHR NSAHSAGVGA IFDRVLTELV SKMRDMQMDK TELGCLRAIV LFNPDSKGLS NPAEVEALRE ...Sequence: SANEDMPVEK ILEAELAVEP KTETYVEANM GLNPSSPNDP VTNICQAADK QLFTLVEWAK RIPHFSELPL DDQVILLRAG WNELLIASAS HRSIAVKDGI LLATGLHVHR NSAHSAGVGA IFDRVLTELV SKMRDMQMDK TELGCLRAIV LFNPDSKGLS NPAEVEALRE KVYASLEAYC KHKYPEQPGR FAKLLLRLPA LRSIGLKCLE HLFFFKLIGD TPIDTFLMEM LEAPHQAT |
Entity #1538 | Name: RXRΔH12 / Type: protein Description: Retinoid-X receptor alpha (RXR-alpha) Δ helix12 Formula weight: 23.5 / Num. of mol.: 1 / Source: Mouse (Mus musculus) / References: UniProt: P28700 Sequence: STSSANEDMP VEKILEAELA VEPKTETYVE ANMGLNPSSP NDPVTNICQA ADKQLFTLVE WAKRIPHFSE LPLDDQVILL RAGWNELLIA SASHRSIAVK DGILLATGLH VHRNSAHSAG VGAIFDRVLT ELVSKMRDMQ MDKTELGCLR AIVLFNPDSK GLSNPAEVEA ...Sequence: STSSANEDMP VEKILEAELA VEPKTETYVE ANMGLNPSSP NDPVTNICQA ADKQLFTLVE WAKRIPHFSE LPLDDQVILL RAGWNELLIA SASHRSIAVK DGILLATGLH VHRNSAHSAG VGAIFDRVLT ELVSKMRDMQ MDKTELGCLR AIVLFNPDSK GLSNPAEVEA LREKVYASLE AYCKHKYPEQ PGRFAKLLLR LPALRSIGLK CLE |
-Experimental information
Beam | Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.099 Å / Dist. spec. to detc.: 2.9 mm | ||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||
Scan | Title: RXRΔH12/RAR Heterodimer : N-CoRNID Complex (1:1) / Measurement date: Jul 23, 2014 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 10 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 686 /
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Result | Type of curve: merged /
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