SASDF34: Free Nuclear receptor CoRepressor NID (spanning from residue Gln2...

基本情報

登録情報

データベース: SASBDB / ID: SASDF34

試料

Free Nuclear receptor CoRepressor NID (spanning from residue Gln2059 to Glu2325)

• Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) (protein), N-CoR-NID, Mouse (Mus musculus)

機能・相同性

分子機能:

Transcriptional regulation of white adipocyte differentiation / NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis / NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux / definitive erythrocyte differentiation / CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / Downregulation of SMAD2/3:SMAD4 transcriptional activity / Nuclear Receptor transcription pathway / HDACs deacetylate histones / Notch-HLH transcription pathway / MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis / Regulation of lipid metabolism by PPARalpha / Cytoprotection by HMOX1 / thalamus development / nuclear retinoic acid receptor binding / regulation of thyroid hormone receptor signaling pathway / nuclear thyroid hormone receptor binding / histone deacetylase regulator activity / locomotor rhythm / cellular response to Thyroglobulin triiodothyronine / regulation of multicellular organism growth / epidermis development / establishment of skin barrier / nuclear retinoid X receptor binding / transcription repressor complex / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / cholesterol homeostasis / circadian regulation of gene expression / transcription corepressor activity / chromatin organization / T cell differentiation in thymus / gene expression / transcription regulator complex / sequence-specific DNA binding / in utero embryonic development / transcription cis-regulatory region binding / protein stabilization / protein domain specific binding / negative regulation of DNA-templated transcription / chromatin binding / regulation of transcription by RNA polymerase II / negative regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / nucleus / cytosol / cytoplasm

ドメイン・相同性:

N-CoR, GPS2-interacting domain / : / G-protein pathway suppressor 2-interacting domain / SANT domain profile. / Myb domain / SANT domain / Myb-like DNA-binding domain / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / SANT/Myb domain / Homeobox-like domain superfamily

構成要素:

Nuclear receptor corepressor 1

• 生物種: Mouse (Mus musculus)
• 引用: ジャーナル: Structure / 年: 2019; タイトル: Interplay of Protein Disorder in Retinoic Acid Receptor Heterodimer and Its Corepressor Regulates Gene Expression.; 著者: Tiago N Cordeiro / Nathalie Sibille / Pierre Germain / Philippe Barthe / Abdelhay Boulahtouf / Fréderic Allemand / Rémy Bailly / Valérie Vivat / Christine Ebel / Alessandro Barducci / William Bourguet / Albane le Maire / Pau Bernadó / ; 要旨: In its unliganded form, the retinoic acid receptor (RAR) in heterodimer with the retinoid X receptor (RXR) exerts a strong repressive activity facilitated by the recruitment of transcriptional corepressors in the promoter region of target genes. By integrating complementary structural, biophysical, and computational information, we demonstrate that intrinsic disorder is a required feature for the precise regulation of RAR activity. We show that structural dynamics of RAR and RXR H12 regions is an essential mechanism for RAR regulation. Unexpectedly we found that, while mainly disordered, the corepressor N-CoR presents evolutionary conserved structured regions involved in transient intramolecular contacts. In the presence of RXR/RAR, N-CoR exploits its multivalency to form a cooperative multisite complex that displays equilibrium between different conformational states that can be tuned by cognate ligands and receptor mutations. This equilibrium is key to preserving the repressive basal state while allowing the conversion to a transcriptionally active form.

登録者

• Tiago Cordeiro (Instituto de Tecnologia Química e Biológica António Xavier, Universidade de Lisboa, Oeiras, Portugal)

モデル

試料

• 試料: 名称: Free Nuclear receptor CoRepressor NID (spanning from residue Gln2059 to Glu2325); 試料濃度: 2 mg/ml
• バッファ: 名称: 50mM Tris HCl, 150mM NaCl, 2mM TCEP. / pH: 7.5

要素 #1515

名称: N-CoR-NID / タイプ: protein
記述: Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID)
分子量: 29.139 / 分子数: 1 / 由来: Mouse (Mus musculus) / 参照: UniProt: Q60974
配列:

GPHMQVPRTH RLITLADHIC QIITQDFARN QVPSQASTST FQTSPSALSS TPVRTKTSSR YSPESQSQTV LHPRPGPRVS PENLVDKSRG SRPGKSPERS HIPSEPYEPI SPPQGPAVHE KQDSMLLLSQ RGVDPAEQRS DSRSPGSISY LPSFFTKLES TSPMVKSKKQ EIFRKLNSSG GGDSDMAAAQ PGTEIFNLPA VTTSGAVSSR SHSFADPASN LGLEDIIRKA LMGSFDDKVE DHGVVMSHPV GIMPGSASTS VVTSSEARRD E

実験情報

• ビーム: 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotron / 波長: 0.099 Å / スペクトロメータ・検出器間距離: 2.9 mm
• 検出器: 名称: Pilatus 1M / タイプ: Dectris / Pixsize x: 172 mm

スキャン

タイトル: Free Nuclear receptor CoRepressor NID (spanning from residue Gln2059 to Glu2325)
測定日: 2016年6月20日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 15 / 単位: 1/nm /

	Min	Max
Q	0.1007	4.9518

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 796 /

	Min	Max
Q	0.119548	3.87118
P(R) point	1	796
R	0	17.7

結果

カーブのタイプ: single_conc
コメント: Several datasets were collected at multiple concentrations ranging between 1.3 and 2.0 mg/ml. Only the data of 2mg/ml is shown.

	実験値	Standard	Standard error	Porod
分子量	26.3 kDa	26.3 kDa	3	-
体積	-	-	-	102 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	27.08	0.04	26.64	0.12
慣性半径, Rg	5.025 nm	0.1	4.72 nm	0.12

	Min	Max	Error
D	-	17.7	0.6
Guinier point	5	37	-