SASDF34: Free Nuclear receptor CoRepressor NID (spanning from residue Gln2...

Basic information

Entry

Database: SASBDB / ID: SASDF34

Sample

Free Nuclear receptor CoRepressor NID (spanning from residue Gln2059 to Glu2325)

• Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) (protein), N-CoR-NID, Mouse (Mus musculus)

Function / homology

Function:

Transcriptional regulation of white adipocyte differentiation / NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis / definitive erythrocyte differentiation / NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux / CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / Downregulation of SMAD2/3:SMAD4 transcriptional activity / Nuclear Receptor transcription pathway / Notch-HLH transcription pathway / Regulation of lipid metabolism by PPARalpha / thalamus development / negative regulation of androgen receptor signaling pathway / Cytoprotection by HMOX1 / nuclear retinoic acid receptor binding / negative regulation of JNK cascade / regulation of thyroid hormone mediated signaling pathway / negative regulation of glycolytic process / peroxisome proliferator activated receptor binding / histone deacetylase regulator activity / nuclear thyroid hormone receptor binding / negative regulation of fatty acid metabolic process / locomotor rhythm / histone deacetylase complex / cellular response to Thyroglobulin triiodothyronine / regulation of multicellular organism growth / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / spindle assembly / epidermis development / establishment of skin barrier / nuclear retinoid X receptor binding / transcription repressor complex / negative regulation of miRNA transcription / cholesterol homeostasis / nuclear estrogen receptor binding / nuclear receptor binding / RNA polymerase II transcription regulatory region sequence-specific DNA binding / circadian regulation of gene expression / mitotic spindle / histone deacetylase binding / transcription corepressor activity / T cell differentiation in thymus / gene expression / chromatin organization / in utero embryonic development / transcription regulator complex / RNA polymerase II-specific DNA-binding transcription factor binding / sequence-specific DNA binding / protein stabilization / transcription cis-regulatory region binding / protein domain specific binding / negative regulation of gene expression / negative regulation of DNA-templated transcription / chromatin binding / positive regulation of cell population proliferation / chromatin / regulation of transcription by RNA polymerase II / perinuclear region of cytoplasm / negative regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / nucleus / cytosol / cytoplasm

Domain/homology:

N-CoR, GPS2-interacting domain / G-protein pathway suppressor 2-interacting domain / SANT domain profile. / SANT domain / Myb domain / Myb-like DNA-binding domain / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / SANT/Myb domain / Homeobox-like domain superfamily

Component:

Nuclear receptor corepressor 1

• Biological species: Mouse (Mus musculus)
• Citation: Journal: Structure / Year: 2019; Title: Interplay of Protein Disorder in Retinoic Acid Receptor Heterodimer and Its Corepressor Regulates Gene Expression.; Authors: Tiago N Cordeiro / Nathalie Sibille / Pierre Germain / Philippe Barthe / Abdelhay Boulahtouf / Fréderic Allemand / Rémy Bailly / Valérie Vivat / Christine Ebel / Alessandro Barducci / William Bourguet / Albane le Maire / Pau Bernadó / ; Abstract: In its unliganded form, the retinoic acid receptor (RAR) in heterodimer with the retinoid X receptor (RXR) exerts a strong repressive activity facilitated by the recruitment of transcriptional corepressors in the promoter region of target genes. By integrating complementary structural, biophysical, and computational information, we demonstrate that intrinsic disorder is a required feature for the precise regulation of RAR activity. We show that structural dynamics of RAR and RXR H12 regions is an essential mechanism for RAR regulation. Unexpectedly we found that, while mainly disordered, the corepressor N-CoR presents evolutionary conserved structured regions involved in transient intramolecular contacts. In the presence of RXR/RAR, N-CoR exploits its multivalency to form a cooperative multisite complex that displays equilibrium between different conformational states that can be tuned by cognate ligands and receptor mutations. This equilibrium is key to preserving the repressive basal state while allowing the conversion to a transcriptionally active form.

Contact author

• Tiago Cordeiro (Instituto de Tecnologia Química e Biológica António Xavier, Universidade de Lisboa, Oeiras, Portugal)

Models

Sample

• Sample: Name: Free Nuclear receptor CoRepressor NID (spanning from residue Gln2059 to Glu2325); Specimen concentration: 2 mg/ml
• Buffer: Name: 50mM Tris HCl, 150mM NaCl, 2mM TCEP. / pH: 7.5

Entity #1515

Name: N-CoR-NID / Type: protein
Description: Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID)
Formula weight: 29.139 / Num. of mol.: 1 / Source: Mouse (Mus musculus) / References: UniProt: Q60974
Sequence:

GPHMQVPRTH RLITLADHIC QIITQDFARN QVPSQASTST FQTSPSALSS TPVRTKTSSR YSPESQSQTV LHPRPGPRVS PENLVDKSRG SRPGKSPERS HIPSEPYEPI SPPQGPAVHE KQDSMLLLSQ RGVDPAEQRS DSRSPGSISY LPSFFTKLES TSPMVKSKKQ EIFRKLNSSG GGDSDMAAAQ PGTEIFNLPA VTTSGAVSSR SHSFADPASN LGLEDIIRKA LMGSFDDKVE DHGVVMSHPV GIMPGSASTS VVTSSEARRD E

Experimental information

• Beam: Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.099 Å / Dist. spec. to detc.: 2.9 mm
• Detector: Name: Pilatus 1M / Type: Dectris / Pixsize x: 172 mm

Scan

Title: Free Nuclear receptor CoRepressor NID (spanning from residue Gln2059 to Glu2325)
Measurement date: Jun 20, 2016 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 1 sec. / Number of frames: 15 / Unit: 1/nm /

	Min	Max
Q	0.1007	4.9518

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 796 /

	Min	Max
Q	0.119548	3.87118
P(R) point	1	796
R	0	17.7

Result

Type of curve: single_conc
Comments: Several datasets were collected at multiple concentrations ranging between 1.3 and 2.0 mg/ml. Only the data of 2mg/ml is shown.

	Experimental	Standard	Standard error	Porod
MW	26.3 kDa	26.3 kDa	3	-
Volume	-	-	-	102 nm3

	P(R)	P(R) error	Guinier	Guinier error
Forward scattering, I0	27.08	0.04	26.64	0.12
Radius of gyration, Rg	5.025 nm	0.1	4.72 nm	0.12

	Min	Max	Error
D	-	17.7	0.6
Guinier point	5	37	-