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-基本情報
登録情報 | データベース: SASBDB / ID: SASDF34 |
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試料 | Free Nuclear receptor CoRepressor NID (spanning from residue Gln2059 to Glu2325)
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機能・相同性 | 機能・相同性情報 Transcriptional regulation of white adipocyte differentiation / NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis / definitive erythrocyte differentiation / NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux / CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / Downregulation of SMAD2/3:SMAD4 transcriptional activity / Nuclear Receptor transcription pathway / HDACs deacetylate histones / Notch-HLH transcription pathway / Regulation of lipid metabolism by PPARalpha ...Transcriptional regulation of white adipocyte differentiation / NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis / definitive erythrocyte differentiation / NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux / CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / Downregulation of SMAD2/3:SMAD4 transcriptional activity / Nuclear Receptor transcription pathway / HDACs deacetylate histones / Notch-HLH transcription pathway / Regulation of lipid metabolism by PPARalpha / thalamus development / Cytoprotection by HMOX1 / nuclear retinoic acid receptor binding / regulation of thyroid hormone mediated signaling pathway / peroxisome proliferator activated receptor binding / histone deacetylase regulator activity / nuclear thyroid hormone receptor binding / locomotor rhythm / cellular response to Thyroglobulin triiodothyronine / regulation of multicellular organism growth / epidermis development / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / establishment of skin barrier / nuclear retinoid X receptor binding / transcription repressor complex / cholesterol homeostasis / nuclear estrogen receptor binding / RNA polymerase II transcription regulatory region sequence-specific DNA binding / circadian regulation of gene expression / histone deacetylase binding / transcription corepressor activity / chromatin organization / T cell differentiation in thymus / 遺伝子発現 / in utero embryonic development / transcription regulator complex / sequence-specific DNA binding / protein stabilization / transcription cis-regulatory region binding / protein domain specific binding / negative regulation of gene expression / negative regulation of DNA-templated transcription / chromatin binding / positive regulation of cell population proliferation / regulation of transcription by RNA polymerase II / perinuclear region of cytoplasm / negative regulation of transcription by RNA polymerase II / DNA binding / 核質 / 細胞核 / 細胞質基質 / 細胞質 類似検索 - 分子機能 |
生物種 | Mouse (Mus musculus) |
引用 | ジャーナル: Structure / 年: 2019 タイトル: Interplay of Protein Disorder in Retinoic Acid Receptor Heterodimer and Its Corepressor Regulates Gene Expression. 著者: Tiago N Cordeiro / Nathalie Sibille / Pierre Germain / Philippe Barthe / Abdelhay Boulahtouf / Fréderic Allemand / Rémy Bailly / Valérie Vivat / Christine Ebel / Alessandro Barducci / ...著者: Tiago N Cordeiro / Nathalie Sibille / Pierre Germain / Philippe Barthe / Abdelhay Boulahtouf / Fréderic Allemand / Rémy Bailly / Valérie Vivat / Christine Ebel / Alessandro Barducci / William Bourguet / Albane le Maire / Pau Bernadó / 要旨: In its unliganded form, the retinoic acid receptor (RAR) in heterodimer with the retinoid X receptor (RXR) exerts a strong repressive activity facilitated by the recruitment of transcriptional ...In its unliganded form, the retinoic acid receptor (RAR) in heterodimer with the retinoid X receptor (RXR) exerts a strong repressive activity facilitated by the recruitment of transcriptional corepressors in the promoter region of target genes. By integrating complementary structural, biophysical, and computational information, we demonstrate that intrinsic disorder is a required feature for the precise regulation of RAR activity. We show that structural dynamics of RAR and RXR H12 regions is an essential mechanism for RAR regulation. Unexpectedly we found that, while mainly disordered, the corepressor N-CoR presents evolutionary conserved structured regions involved in transient intramolecular contacts. In the presence of RXR/RAR, N-CoR exploits its multivalency to form a cooperative multisite complex that displays equilibrium between different conformational states that can be tuned by cognate ligands and receptor mutations. This equilibrium is key to preserving the repressive basal state while allowing the conversion to a transcriptionally active form. |
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-構造の表示
-ダウンロードとリンク
-モデル
-試料
試料 | 名称: Free Nuclear receptor CoRepressor NID (spanning from residue Gln2059 to Glu2325) 試料濃度: 2 mg/ml |
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バッファ | 名称: 50mM Tris HCl, 150mM NaCl, 2mM TCEP. / pH: 7.5 |
要素 #1515 | 名称: N-CoR-NID / タイプ: protein 記述: Nuclear receptor CoRepressor 1; Nuclear Receptor Interaction Domain (NID) 分子量: 29.139 / 分子数: 1 / 由来: Mouse (Mus musculus) / 参照: UniProt: Q60974 配列: GPHMQVPRTH RLITLADHIC QIITQDFARN QVPSQASTST FQTSPSALSS TPVRTKTSSR YSPESQSQTV LHPRPGPRVS PENLVDKSRG SRPGKSPERS HIPSEPYEPI SPPQGPAVHE KQDSMLLLSQ RGVDPAEQRS DSRSPGSISY LPSFFTKLES TSPMVKSKKQ ...配列: GPHMQVPRTH RLITLADHIC QIITQDFARN QVPSQASTST FQTSPSALSS TPVRTKTSSR YSPESQSQTV LHPRPGPRVS PENLVDKSRG SRPGKSPERS HIPSEPYEPI SPPQGPAVHE KQDSMLLLSQ RGVDPAEQRS DSRSPGSISY LPSFFTKLES TSPMVKSKKQ EIFRKLNSSG GGDSDMAAAQ PGTEIFNLPA VTTSGAVSSR SHSFADPASN LGLEDIIRKA LMGSFDDKVE DHGVVMSHPV GIMPGSASTS VVTSSEARRD E |
-実験情報
ビーム | 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotronシンクロトロン / 波長: 0.099 Å / スペクトロメータ・検出器間距離: 2.9 mm | ||||||||||||||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 1M / タイプ: Dectris / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||||||||||||||
スキャン | タイトル: Free Nuclear receptor CoRepressor NID (spanning from residue Gln2059 to Glu2325) 測定日: 2016年6月20日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 15 / 単位: 1/nm /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 5.0 / ポイント数: 796 /
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結果 | カーブのタイプ: single_conc コメント: Several datasets were collected at multiple concentrations ranging between 1.3 and 2.0 mg/ml. Only the data of 2mg/ml is shown.
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