+
データを開く
-
基本情報
登録情報 | データベース: SASBDB / ID: SASDEV6 |
---|---|
![]() | KRAB-associated protein 1 (KAP1); TRIM28; full length protein
|
機能・相同性 | ![]() convergent extension involved in axis elongation / : / Krueppel-associated box domain binding / embryonic placenta morphogenesis / positive regulation of DNA methylation-dependent heterochromatin formation / suppression of viral release by host / epigenetic programming of gene expression / negative regulation of single stranded viral RNA replication via double stranded DNA intermediate / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() 類似検索 - 分子機能 |
生物種 | ![]() ![]() |
![]() | ![]() タイトル: KAP1 is an antiparallel dimer with a functional asymmetry. 著者: Giulia Fonti / Maria J Marcaida / Louise C Bryan / Sylvain Träger / Alexandra S Kalantzi / Pierre-Yves Jl Helleboid / Davide Demurtas / Mark D Tully / Sergei Grudinin / Didier Trono / Beat ...著者: Giulia Fonti / Maria J Marcaida / Louise C Bryan / Sylvain Träger / Alexandra S Kalantzi / Pierre-Yves Jl Helleboid / Davide Demurtas / Mark D Tully / Sergei Grudinin / Didier Trono / Beat Fierz / Matteo Dal Peraro / ![]() ![]() 要旨: KAP1 (KRAB domain-associated protein 1) plays a fundamental role in regulating gene expression in mammalian cells by recruiting different transcription factors and altering the chromatin state. In ...KAP1 (KRAB domain-associated protein 1) plays a fundamental role in regulating gene expression in mammalian cells by recruiting different transcription factors and altering the chromatin state. In doing so, KAP1 acts both as a platform for macromolecular interactions and as an E3 small ubiquitin modifier ligase. This work sheds light on the overall organization of the full-length protein combining solution scattering data, integrative modeling, and single-molecule experiments. We show that KAP1 is an elongated antiparallel dimer with an asymmetry at the C-terminal domains. This conformation is consistent with the finding that the Really Interesting New Gene (RING) domain contributes to KAP1 auto-SUMOylation. Importantly, this intrinsic asymmetry has key functional implications for the KAP1 network of interactions, as the heterochromatin protein 1 (HP1) occupies only one of the two putative HP1 binding sites on the KAP1 dimer, resulting in an unexpected stoichiometry, even in the context of chromatin fibers. |
![]() |
|
-
構造の表示
構造ビューア | 分子: ![]() ![]() |
---|
-
ダウンロードとリンク
-モデル
モデル #2557 | ![]() タイプ: dummy / ダミー原子の半径: 1.90 A / 対称性 ![]() ![]() |
---|---|
モデル #2558 | ![]() タイプ: atomic / 対称性 ![]() ![]() |
-
試料
![]() | 名称: KRAB-associated protein 1 (KAP1); TRIM28; full length protein 試料濃度: 15 mg/ml |
---|---|
バッファ | 名称: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP / pH: 7.5 |
要素 #1350 | 名称: KAP1FL / タイプ: protein 記述: Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28 分子量: 91.542 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: Q13263 配列: MGSSHHHHHH SQDPNSSSEN LYFQGAAASA AAASAAAASA AAASAGSPGP GEGSAGGEKR STAPSAAASA SASAAASSPA GGGAEALELL EHCGVCRERL RPEREPRLLP CLHSACSACL GPAAPAAANS SGDGGAAGDG TVVDCPVCKQ QCFSKDIVEN YFMRDSGSKA ...配列: MGSSHHHHHH SQDPNSSSEN LYFQGAAASA AAASAAAASA AAASAGSPGP GEGSAGGEKR STAPSAAASA SASAAASSPA GGGAEALELL EHCGVCRERL RPEREPRLLP CLHSACSACL GPAAPAAANS SGDGGAAGDG TVVDCPVCKQ QCFSKDIVEN YFMRDSGSKA ATDAQDANQC CTSCEDNAPA TSYCVECSEP LCETCVEAHQ RVKYTKDHTV RSTGPAKSRD GERTVYCNVH KHEPLVLFCE SCDTLTCRDC QLNAHKDHQY QFLEDAVRNQ RKLLASLVKR LGDKHATLQK STKEVRSSIR QVSDVQKRVQ VDVKMAILQI MKELNKRGRV LVNDAQKVTE GQQERLERQH WTMTKIQKHQ EHILRFASWA LESDNNTALL LSKKLIYFQL HRALKMIVDP VEPHGEMKFQ WDLNAWTKSA EAFGKIVAER PGTNSTGPAP MAPPRAPGPL SKQGSGSSQP MEVQEGYGFG SGDDPYSSAE PHVSGVKRSR SGEGEVSGLM RKVPRVSLER LDLDLTADSQ PPVFKVFPGS TTEDYNLIVI ERGAAAAATG QPGTAPAGTP GAPPLAGMAI VKEEETEAAI GAPPTATEGP ETKPVLMALA EGPGAEGPRL ASPSGSTSSG LEVVAPEGTS APGGGPGTLD DSATICRVCQ KPGDLVMCNQ CEFCFHLDCH LPALQDVPGE EWSCSLCHVL PDLKEEDGSL SLDGADSTGV VAKLSPANQR KCERVLLALF CHEPCRPLHQ LATDSTFSLD QPGGTLDLTL IRARLQEKLS PPYSSPQEFA QDVGRMFKQF NKLTEDKADV QSIIGLQRFF ETRMNEAFGD TKFSAVLVEP PPMSLPGAGL SSQELSGGPG DGP |
-実験情報
ビーム | 設備名称: ESRF BM29 / 地域: Grenoble / 国: France ![]() ![]() | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
検出器 | 名称: Pilatus 1M / タイプ: Dectris / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||
スキャン | 測定日: 2017年7月6日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 1 sec. / 単位: 1/nm /
| ||||||||||||||||||||||||||||||
距離分布関数 P(R) |
| ||||||||||||||||||||||||||||||
結果 |
|