SASDEE2: The N-terminal domain of estrogen receptor alpha (ER-NTD)

Basic information

• Entry: Database: SASBDB / ID: SASDEE2

Sample

The N-terminal domain of estrogen receptor alpha

• The N-terminal domain of estrogen receptor alpha (protein), ER-NTD, Homo sapiens

Function / homology

Function:

regulation of epithelial cell apoptotic process / antral ovarian follicle growth / G protein-coupled estrogen receptor activity / regulation of branching involved in prostate gland morphogenesis / RUNX1 regulates transcription of genes involved in WNT signaling / RUNX1 regulates estrogen receptor mediated transcription / regulation of toll-like receptor signaling pathway / prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis / nuclear estrogen receptor activity / epithelial cell proliferation involved in mammary gland duct elongation / prostate epithelial cord elongation / epithelial cell development / negative regulation of smooth muscle cell apoptotic process / mammary gland branching involved in pregnancy / uterus development / vagina development / androgen metabolic process / TFIIB-class transcription factor binding / steroid hormone mediated signaling pathway / mammary gland alveolus development / intracellular estrogen receptor signaling pathway / cellular response to estrogen stimulus / estrogen response element binding / positive regulation of phospholipase C activity / intracellular steroid hormone receptor signaling pathway / negative regulation of canonical NF-kappaB signal transduction / Nuclear signaling by ERBB4 / RNA polymerase II preinitiation complex assembly / protein localization to chromatin / TBP-class protein binding / TFAP2 (AP-2) family regulates transcription of growth factors and their receptors / steroid binding / nitric-oxide synthase regulator activity / ESR-mediated signaling / 14-3-3 protein binding / transcription corepressor binding / negative regulation of miRNA transcription / cellular response to estradiol stimulus / transcription coregulator binding / stem cell differentiation / nuclear estrogen receptor binding / positive regulation of nitric-oxide synthase activity / euchromatin / SUMOylation of intracellular receptors / negative regulation of DNA-binding transcription factor activity / transcription coactivator binding / beta-catenin binding / Nuclear Receptor transcription pathway / response to estrogen / male gonad development / Constitutive Signaling by Aberrant PI3K in Cancer / Regulation of RUNX2 expression and activity / nuclear receptor activity / positive regulation of nitric oxide biosynthetic process / positive regulation of DNA-binding transcription factor activity / positive regulation of fibroblast proliferation / Ovarian tumor domain proteases / sequence-specific double-stranded DNA binding / response to estradiol / PIP3 activates AKT signaling / phospholipase C-activating G protein-coupled receptor signaling pathway / ATPase binding / positive regulation of cytosolic calcium ion concentration / fibroblast proliferation / regulation of inflammatory response / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / DNA-binding transcription activator activity, RNA polymerase II-specific / Estrogen-dependent gene expression / transcription regulator complex / Extra-nuclear estrogen signaling / calmodulin binding / DNA-binding transcription factor activity, RNA polymerase II-specific / chromatin remodeling / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of gene expression / chromatin binding / chromatin / regulation of DNA-templated transcription / regulation of transcription by RNA polymerase II / protein kinase binding / positive regulation of DNA-templated transcription / Golgi apparatus / negative regulation of transcription by RNA polymerase II / enzyme binding / signal transduction / positive regulation of transcription by RNA polymerase II / protein-containing complex / zinc ion binding / nucleoplasm / membrane / identical protein binding / nucleus / plasma membrane / cytosol / cytoplasm

Domain/homology:

Estrogen receptor / Oestrogen-type nuclear receptor final C-terminal domain / : / Oestrogen receptor / Oestrogen-type nuclear receptor final C-terminal / Estrogen receptor/oestrogen-related receptor / Nuclear hormone receptor / Nuclear hormones receptors DNA-binding region signature. / Zinc finger, nuclear hormone receptor-type / Zinc finger, C4 type (two domains) / Nuclear hormone receptors DNA-binding domain profile. / c4 zinc finger in nuclear hormone receptors / Nuclear hormone receptor, ligand-binding domain / Nuclear hormone receptor-like domain superfamily / Ligand-binding domain of nuclear hormone receptor / Nuclear receptor (NR) ligand-binding (LBD) domain profile. / Ligand binding domain of hormone receptors / Zinc finger, NHR/GATA-type

Component:

Estrogen receptor

• Biological species: Homo sapiens (human)
• Citation: Date: 2018 Dec; Title: A Metastable Contact and Structural Disorder in the Estrogen Receptor Transactivation Domain; Authors: Peng Y / Cao S / Kiselar J / Xiao X / Du Z / Hsieh A / Ko S / Chen Y / Agrawal P / Zheng W / Shi W / Jiang W / Yang L / Chance M / Surewicz W / Buck M

Contact author

• Sichun Yang (Case Western Reserve University)

Models

• Model #2229: ; Type: atomic / Chi-square value: 0.136161 / P-value: 0.002500; Search similar-shape structures of this assembly by Omokage search (details)
• Model #2230: ; Type: atomic / Chi-square value: 0.341056 / P-value: 0.002500; Search similar-shape structures of this assembly by Omokage search (details)
• Model #2231: ; Type: atomic / Chi-square value: 0.509796 / P-value: 0.002500; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: The N-terminal domain of estrogen receptor alpha / Specimen concentration: 2.5 mg/ml
• Buffer: Name: 20 mM sodium phosphate, 50 mM NaCl, 0.05 mM TCEP / pH: 7.4

Entity #1227

Name: ER-NTD / Type: protein
Description: The N-terminal domain of estrogen receptor alpha
Formula weight: 20.175 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P03372
Sequence:

SNAMTMTLHT KASGMALLHQ IQGNELEPLN RPQLKIPLER PLGEVYLDSS KPAVYNYPEG AAYEFNAAAA ANAQVYGQTG LPYGPGSEAA AFGSNGLGGF PPLNSVSPSP LMLLHPPPQL SPFLQPHGQQ VPYYLENEPS GYTVREAGPP AFYRPNSDNR RQGGRERLAS TNDKGSMAME SAKETRY

Experimental information

• Beam: Instrument name: National Synchrotron Light Source (NSLS-II) 16-ID (LiX); City: Brookhaven, NY / 国: USA / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.09 Å / Dist. spec. to detc.: 3 mm
• Detector: Name: Pilatus3 S 1M / Pixsize x: 172 mm

Scan

Title: The N-terminal domain of estrogen receptor alpha / Measurement date: Jun 12, 2017 / Cell temperature: 10 °C / Exposure time: 3 sec. / Number of frames: 3 / Unit: 1/A /

	Min	Max
Q	0.029	0.265

Distance distribution function P(R)

Sofotware P(R): GNOM 4.6 / Number of points: 79 /

	Min	Max
Q	0.03	0.265
P(R) point	1	79
R	0	100

Result

Experimental MW: 20.18 kDa / Type of curve: single_conc

	P(R)	Guinier	Guinier error
Forward scattering, I0	9.237	9.21	0.1
Radius of gyration, Rg	3.11 nm	3 nm	0.16

	Min	Max
D	-	10
Guinier point	3	15