SASDE48: Toxin GraT (Putative killer protein, Toxin GraT., GraT)

基本情報

登録情報

データベース: SASBDB / ID: SASDE48

試料

Toxin GraT

• Putative killer protein, Toxin GraT. (protein), GraT, Pseudomonas putida

• 機能・相同性: Toxin HigB-1 / RelE-like toxin of type II toxin-antitoxin system HigB / Toxin-antitoxin system, RelE/ParE toxin domain superfamily / molecular function activator activity / Killer protein
• 生物種: Pseudomonas putida (バクテリア)
• 引用: ジャーナル: Nat Commun / 年: 2019; タイトル: A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.; 著者: Ariel Talavera / Hedvig Tamman / Andres Ainelo / Albert Konijnenberg / San Hadži / Frank Sobott / Abel Garcia-Pino / Rita Hõrak / Remy Loris / ; 要旨: Bacterial toxin-antitoxin (TA) modules are tightly regulated to maintain growth in favorable conditions or growth arrest during stress. A typical regulatory strategy involves the antitoxin binding and repressing its own promoter while the toxin often acts as a co-repressor. Here we show that Pseudomonas putida graTA-encoded antitoxin GraA and toxin GraT differ from other TA proteins in the sense that not the antitoxin but the toxin possesses a flexible region. GraA auto-represses the graTA promoter: two GraA dimers bind cooperatively at opposite sides of the operator sequence. Contrary to other TA modules, GraT is a de-repressor of the graTA promoter as its N-terminal disordered segment prevents the binding of the GraTA complex to the operator. Removal of this region restores operator binding and abrogates Gr aT toxicity. GraTA represents a TA module where a flexible region in the toxin rather than in the antitoxin controls operon expression and toxin activity.

登録者

• Ariel Talavera (VUB, Vrije Universiteit Brussel, Pleinlaan 2 1050 Brussel)

モデル

試料

• 試料: 名称: Toxin GraT / 試料濃度: 12.5 mg/ml
• バッファ: 名称: 50 mM Tris 250 mM NaCl 2 mM TCEP / pH: 8

要素 #1379

名称: GraT / タイプ: protein / 記述: Putative killer protein, Toxin GraT. / 分子量: 11.104 / 分子数: 1 / 由来: Pseudomonas putida / 参照: UniProt: Q88MI5
配列:

MIRSFSCADT EALFTTGKTR RGSDIKSVAE RKLAMLDAAT ELRDLRSPPG NRLESLSGNR ADQHSIRVND QWRLCFTWTE HGPVNVEIVD YENLYFQ

実験情報

• ビーム: 設備名称: SOLEIL SWING / 地域: Saint-Aubin / 国: France / 線源: X-ray synchrotron / 波長: 0.103317 Å / スペクトロメータ・検出器間距離: 1.499 mm
• 検出器: 名称: Eiger 4M / Pixsize x: 75 mm

スキャン

タイトル: Toxin GraT / 測定日: 2018年9月21日 / 保管温度: 24.8 °C / セル温度: 25 °C / 照射時間: 0.99 sec. / フレーム数: 40 / 単位: 1/A /

	Min	Max
Q	0.0146	0.6642

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 825 /

	Min	Max
Q	0.0273667	0.526995
P(R) point	1	825
R	0	54.66

結果

カーブのタイプ: sec /

	実験値	Porod
分子量	11.1 kDa	-
体積	-	18 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	0.0143	2.0E-5	0.01421	1.5E-5
慣性半径, Rg	1.55 nm	0.3	1.509 nm	0.03

	Min	Max
D	-	5.47
Guinier point	38	122