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TitleA dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 972, Year 2019
Publish dateFeb 27, 2019
AuthorsAriel Talavera / Hedvig Tamman / Andres Ainelo / Albert Konijnenberg / San Hadži / Frank Sobott / Abel Garcia-Pino / Rita Hõrak / Remy Loris /
PubMed AbstractBacterial toxin-antitoxin (TA) modules are tightly regulated to maintain growth in favorable conditions or growth arrest during stress. A typical regulatory strategy involves the antitoxin binding ...Bacterial toxin-antitoxin (TA) modules are tightly regulated to maintain growth in favorable conditions or growth arrest during stress. A typical regulatory strategy involves the antitoxin binding and repressing its own promoter while the toxin often acts as a co-repressor. Here we show that Pseudomonas putida graTA-encoded antitoxin GraA and toxin GraT differ from other TA proteins in the sense that not the antitoxin but the toxin possesses a flexible region. GraA auto-represses the graTA promoter: two GraA dimers bind cooperatively at opposite sides of the operator sequence. Contrary to other TA modules, GraT is a de-repressor of the graTA promoter as its N-terminal disordered segment prevents the binding of the GraTA complex to the operator. Removal of this region restores operator binding and abrogates Gr aT toxicity. GraTA represents a TA module where a flexible region in the toxin rather than in the antitoxin controls operon expression and toxin activity.
External linksNat Commun / PubMed:30814507 / PubMed Central
MethodsSAS (X-ray synchrotron) / X-ray diffraction
Resolution2.002 - 3.8 Å
Structure data

SASDE48: Toxin GraT (Putative killer protein, Toxin GraT., GraT)
Method: SAXS/SANS

PDB-6f8h:
antitoxin GraA
Method: X-RAY DIFFRACTION / Resolution: 2.002 Å

PDB-6f8s:
Toxin-Antitoxin complex GraTA
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-6fix:
antitoxin GraA in complex with its operator
Method: X-RAY DIFFRACTION / Resolution: 3.8 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • pseudomonas putida (bacteria)
  • pseudomonas putida (strain atcc 47054 / dsm 6125 / ncimb 11950 / kt2440) (bacteria)
KeywordsANTITOXIN / GraA / HigA / TOXIN / GraT / HigB / GraTA / HigBA / operator / DNA

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