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Open data
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Basic information
Entry | ![]() |
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![]() | Toxin GraT
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Function / homology | Toxin HigB-1 / RelE-like toxin of type II toxin-antitoxin system HigB / Toxin-antitoxin system, RelE/ParE toxin domain superfamily / molecular function activator activity / Putative Killer protein![]() |
Biological species | ![]() |
![]() | ![]() Title: A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Authors: Ariel Talavera / Hedvig Tamman / Andres Ainelo / Albert Konijnenberg / San Hadži / Frank Sobott / Abel Garcia-Pino / Rita Hõrak / Remy Loris / ![]() ![]() ![]() ![]() Abstract: Bacterial toxin-antitoxin (TA) modules are tightly regulated to maintain growth in favorable conditions or growth arrest during stress. A typical regulatory strategy involves the antitoxin binding ...Bacterial toxin-antitoxin (TA) modules are tightly regulated to maintain growth in favorable conditions or growth arrest during stress. A typical regulatory strategy involves the antitoxin binding and repressing its own promoter while the toxin often acts as a co-repressor. Here we show that Pseudomonas putida graTA-encoded antitoxin GraA and toxin GraT differ from other TA proteins in the sense that not the antitoxin but the toxin possesses a flexible region. GraA auto-represses the graTA promoter: two GraA dimers bind cooperatively at opposite sides of the operator sequence. Contrary to other TA modules, GraT is a de-repressor of the graTA promoter as its N-terminal disordered segment prevents the binding of the GraTA complex to the operator. Removal of this region restores operator binding and abrogates Gr aT toxicity. GraTA represents a TA module where a flexible region in the toxin rather than in the antitoxin controls operon expression and toxin activity. |
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Structure visualization
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Downloads & links
-Data source
SASBDB page | ![]() |
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-Related structure data
Related structure data | ![]() 6f8hC ![]() 6f8sC ![]() 6fixC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
-Models
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Sample
![]() | Name: Toxin GraT / Specimen concentration: 12.5 mg/ml |
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Buffer | Name: 50 mM Tris 250 mM NaCl 2 mM TCEP / pH: 8 |
Entity #1379 | Name: GraT / Type: protein / Description: Putative killer protein, Toxin GraT. / Formula weight: 11.104 / Num. of mol.: 1 / Source: Pseudomonas putida / References: UniProt: Q88MI5 Sequence: MIRSFSCADT EALFTTGKTR RGSDIKSVAE RKLAMLDAAT ELRDLRSPPG NRLESLSGNR ADQHSIRVND QWRLCFTWTE HGPVNVEIVD YENLYFQ |
-Experimental information
Beam | Instrument name: SOLEIL SWING ![]() ![]() | |||||||||||||||||||||||||||||||||
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Detector | Name: Eiger 4M / Pixsize x: 75 mm | |||||||||||||||||||||||||||||||||
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Distance distribution function P(R) |
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