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- SASDDG8: Integrin beta4, fragment of the cytoplasmic region that includes ... -

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Basic information

Entry
Database: SASBDB / ID: SASDDG8
SampleIntegrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463A
  • Integrin beta-4 (1436-1666) R1463A (protein), B4 CS-FnIII-3,4, Homo sapiens
Function / homologyIsoform Beta-4A of Integrin beta-4
Function and homology information
Biological speciesHomo sapiens (human)
CitationDate: 2018 Aug 31
Title: Integrin α6β4 recognition of a linear motif of bullous pemphigoid antigen BP230 controls its recruitment to hemidesmosomes: Supplemental figures S1 to S10 and table S1
Authors: Manso J / Gómez-Hernández M / Carabias A / Alonso-García N / García-Rubio I / Kreft M / Sonnenberg A
Contact author
  • Jose M de Pereda (USAL, La Universidad de Salamanca, Salamanca, Spain)

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Structure visualization

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Models

Model #2064
Type: dummy / Software: (r9988) / Radius of dummy atoms: 1.50 A / Chi-square value: 0.988 / P-value: 0.665832
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463A
Specimen concentration: 0.90-7.10
BufferName: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT
Concentration: 20.00 mM / pH: 7.5 / Composition: 150 mM NaCl, 5% glycerol, 3 mM DTT
Entity #1101Name: B4 CS-FnIII-3,4 / Type: protein / Description: Integrin beta-4 (1436-1666) R1463A / Formula weight: 25.52 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P16144-2
Sequence: GSHMLPRDYS TLTSVSSHDS RLTAGVPDTP TALVFSALGP TSLRVSWQEP RCERPLQGYS VEYQLLNGGE LHRLNIPNPA QTSVVVEDLL PNHSYVFRVR AQSQEGWGRE REGVITIESQ VHPQSPLCPL PGSAFTLSTP SAPGPLVFTA LSPDSLQLSW ERPRRPNGDI ...Sequence:
GSHMLPRDYS TLTSVSSHDS RLTAGVPDTP TALVFSALGP TSLRVSWQEP RCERPLQGYS VEYQLLNGGE LHRLNIPNPA QTSVVVEDLL PNHSYVFRVR AQSQEGWGRE REGVITIESQ VHPQSPLCPL PGSAFTLSTP SAPGPLVFTA LSPDSLQLSW ERPRRPNGDI VGYLVTCEMA QGGGPATAFR VDGDSPESRL TVPGLSENVP YKFKVQARTT EGFGPEREGI ITIES

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Experimental information

BeamInstrument name: PETRA III EMBL P12 / City: Hamburg / : Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3.1 mm
DetectorName: Pilatus 2M
Scan
Title: Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463A
Measurement date: Nov 26, 2013 / Cell temperature: 10 °C / Exposure time: 0.05 sec. / Number of frames: 30 / Unit: 1/nm /
MinMax
Q0.0565 4.4504
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1120 /
MinMax
Q0.0564852 3.00069
P(R) point1 1120
R0 9.5
Result
Type of curve: merged
Comments: 'Synchrotron SAXS data from solutions of a fragment of the cytoplasmic region of the human integrin Beta4 subunit. This fragment includes the final part of a region named the
ExperimentalPorod
MW22.71 kDa24.1 kDa
Volume-36.1 nm3

P(R)Guinier
Forward scattering, I00.01637 0.0164
Radius of gyration, Rg2.444 nm2.417 nm

MinMax
D-9.5
Guinier point1 184

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