SASDDC8: Periplasmic domain of inner membrane protein GspL, tetramer

Basic information

Entry

Database: SASBDB / ID: SASDDC8

Sample

Periplasmic domain of inner membrane protein GspL, tetramer

• Type II secretion system protein L, periplasmic domain (protein), GspLperi, Pseudomonas aeruginosa

Function / homology

Function:

Gram-negative-bacterium-type cell wall / protein secretion by the type II secretion system / type II protein secretion system complex / plasma membrane

Domain/homology:

Type II secretion system protein GspL / GspL, cytoplasmic actin-ATPase-like domain / GspL periplasmic domain / Type II secretion system (T2SS), protein L / GspL periplasmic domain / ATPase, nucleotide binding domain

Component:

Type II secretion system protein L

• Biological species: Pseudomonas aeruginosa (bacteria)
• Citation: Date: 2018 Dec; Title: Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa; Authors: Fulara A / Vandenberghe I / Read R / Devreese B

Contact author

• Aleksandra Fulara (Ghent University, Gent, Belgium)

Models

Sample

• Sample: Name: Periplasmic domain of inner membrane protein GspL, tetramer
• Buffer: Name: 50 mM TRIS, 100 mM NaCl / pH: 7.5 / Comment: pH=7.5 at 25°C

Entity #1111

Name: GspLperi / Type: protein
Description: Type II secretion system protein L, periplasmic domain
Formula weight: 13.966 / Num. of mol.: 2 / Source: Pseudomonas aeruginosa / References: UniProt: P25060
Sequence:

WSHPQFEKGG GDRYAAQSAE LYRQLFPEDR KLINLRAQFD QHLADSASSG GEGQLLGLLG QAATVIGGEP TVSVEQLDFS AARGDVALQV RAPGFDVLER LRSRLSESGL AVQLGSASRD GSTVSARLVI GG

Experimental information

• Beam: Instrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.1033 Å
• Detector: Name: AVIEX PCCD170170 / Type: CCD

Scan

Title: Periplasmic domain of inner membrane protein GspL, tetramer
Measurement date: Apr 8, 2016 / Cell temperature: 15 °C / Number of frames: 15 / Unit: 1/A /

	Min	Max
Q	0.0068	0.6139

Distance distribution function P(R)

Sofotware P(R): GNOM 4.6 / Number of points: 432 /

	Min	Max
Q	0.02868	0.5166
P(R) point	39	470
R	0	105

Result

D max: 10.5 / Type of curve: sec /

	Experimental	Standard
MW	56 kDa	57 kDa

	P(R)	Guinier	Guinier error
Forward scattering, I0	0.03726	0.0379622	2.3E-5
Radius of gyration, Rg	3.098 nm	3.175 nm	0.37