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- SASDDB8: Periplasmic domain of inner membrane protein GspL, dimer -

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Basic information

Entry
Database: SASBDB / ID: SASDDB8
SamplePeriplasmic domain of inner membrane protein GspL, dimer
  • Type II secretion system protein L, periplasmic domain (protein), GspLperi, Pseudomonas aeruginosa
Function / homology
Function and homology information


Gram-negative-bacterium-type cell wall / protein secretion by the type II secretion system / type II protein secretion system complex / plasma membrane
Similarity search - Function
Type II secretion system protein GspL / GspL, cytoplasmic actin-ATPase-like domain / GspL periplasmic domain / Type II secretion system (T2SS), protein L / GspL periplasmic domain / ATPase, nucleotide binding domain
Similarity search - Domain/homology
Type II secretion system protein L
Similarity search - Component
Biological speciesPseudomonas aeruginosa (bacteria)
CitationDate: 2018 Dec
Title: Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa
Authors: Fulara A / Vandenberghe I / Read R / Devreese B
Contact author
  • Aleksandra Fulara (Ghent University, Gent, Belgium)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2072
Type: mix / Radius of dummy atoms: 1.90 A / Symmetry: none / Chi-square value: 5.788 / P-value: 0.000794
Search similar-shape structures of this assembly by Omokage search (details)
Model #2073
Type: mix / Radius of dummy atoms: 1.90 A / Chi-square value: 5.788 / P-value: 0.000794
Search similar-shape structures of this assembly by Omokage search (details)
Model #2074
Type: mix / Radius of dummy atoms: 1.90 A / Chi-square value: 5.788 / P-value: 0.000794
Search similar-shape structures of this assembly by Omokage search (details)
Model #2075
Type: mix / Radius of dummy atoms: 1.90 A / Chi-square value: 5.788 / P-value: 0.000794
Search similar-shape structures of this assembly by Omokage search (details)
Model #2076
Type: mix / Radius of dummy atoms: 1.90 A / Chi-square value: 5.788 / P-value: 0.000794
Search similar-shape structures of this assembly by Omokage search (details)
Model #2077
Type: mix / Radius of dummy atoms: 1.90 A / Chi-square value: 5.788 / P-value: 0.000794
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Periplasmic domain of inner membrane protein GspL, dimer
Specimen concentration: 2.00-13.70
BufferName: 50 mM TRIS, 100 mM NaCl / pH: 7.5 / Comment: pH=7.5 at 25°C
Entity #1111Name: GspLperi / Type: protein
Description: Type II secretion system protein L, periplasmic domain
Formula weight: 13.966 / Num. of mol.: 2 / Source: Pseudomonas aeruginosa / References: UniProt: P25060
Sequence:
WSHPQFEKGG GDRYAAQSAE LYRQLFPEDR KLINLRAQFD QHLADSASSG GEGQLLGLLG QAATVIGGEP TVSVEQLDFS AARGDVALQV RAPGFDVLER LRSRLSESGL AVQLGSASRD GSTVSARLVI GG

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Experimental information

BeamInstrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.1033 Å
DetectorName: AVIEX PCCD170170 / Type: CCD
Scan
Title: Periplasmic domain of inner membrane protein GspL, dimer
Measurement date: Apr 8, 2016 / Storage temperature: 4 °C / Cell temperature: 15 °C / Number of frames: 19 / Unit: 1/A /
MinMax
Q0.008 0.6139
Distance distribution function P(R)
Sofotware P(R): GNOM 4.6 / Number of points: 426 /
MinMax
Q0.0355 0.5166
P(R) point51 476
R0 75
Result
D max: 7.5 / Type of curve: sec /
ExperimentalStandard
MW22.8 kDa36.4 kDa

P(R)GuinierGuinier error
Forward scattering, I00.03865 0.0391339 2.5E-5
Radius of gyration, Rg2.21 nm2.243 nm0.07

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