+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDDC3 |
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試料 | Human mitochondrial cysteine desulfurase-ISCU (NFS1, ISD11, Acp and ISCU heterodimer complex)
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機能・相同性 | 機能・相同性情報 negative regulation of iron ion import across plasma membrane / molybdopterin cofactor metabolic process / Molybdenum cofactor biosynthesis / [4Fe-4S] cluster assembly / iron incorporation into metallo-sulfur cluster / L-cysteine desulfurase complex / Mitochondrial iron-sulfur cluster biogenesis / mitochondrial [2Fe-2S] assembly complex / positive regulation of aconitate hydratase activity / positive regulation of mitochondrial electron transport, NADH to ubiquinone ...negative regulation of iron ion import across plasma membrane / molybdopterin cofactor metabolic process / Molybdenum cofactor biosynthesis / [4Fe-4S] cluster assembly / iron incorporation into metallo-sulfur cluster / L-cysteine desulfurase complex / Mitochondrial iron-sulfur cluster biogenesis / mitochondrial [2Fe-2S] assembly complex / positive regulation of aconitate hydratase activity / positive regulation of mitochondrial electron transport, NADH to ubiquinone / cysteine desulfurase / cysteine desulfurase activity / Mo-molybdopterin cofactor biosynthetic process / iron-sulfur cluster assembly complex / [2Fe-2S] cluster assembly / iron-sulfur cluster assembly / lipid biosynthetic process / lipid A biosynthetic process / iron-sulfur cluster binding / phosphopantetheine binding / acyl binding / acyl carrier activity / ferrous iron binding / 2 iron, 2 sulfur cluster binding / fatty acid biosynthetic process / pyridoxal phosphate binding / Maturation of replicase proteins / intracellular iron ion homeostasis / molecular adaptor activity / nuclear body / ミトコンドリアマトリックス / response to xenobiotic stimulus / iron ion binding / 中心体 / lipid binding / protein homodimerization activity / ミトコンドリア / zinc ion binding / 核質 / metal ion binding / 細胞核 / 細胞質基質 / 細胞質 類似検索 - 分子機能 |
生物種 | Homo sapiens (ヒト) Escherichia coli (strain K12) (大腸菌) |
引用 | ジャーナル: Structure / 年: 2018 タイトル: Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. 著者: Kai Cai / Ronnie O Frederick / Hesam Dashti / John L Markley / 要旨: Cysteine desulfurase plays a central role in mitochondrial iron-sulfur cluster biogenesis by generating sulfur through the conversion of L-cysteine to L-alanine and by serving as the platform for ...Cysteine desulfurase plays a central role in mitochondrial iron-sulfur cluster biogenesis by generating sulfur through the conversion of L-cysteine to L-alanine and by serving as the platform for assembling other components of the biosynthetic machinery, including ISCU, frataxin, and ferredoxin. The human mitochondrial cysteine desulfurase complex consists of two copies each of NFS1, ISD11, and acyl carrier protein. We describe results from chemical crosslinking coupled with tandem mass spectrometry and small-angle X-ray scattering studies that are consistent with a closed NFS1 dimer rather than an open one for both the cysteine desulfurase-ISCU and cysteine desulfurase-ISCU-frataxin complexes. We present a structural model for the cysteine desulfurase-ISCU-frataxin complex derived from chemical crosslinking restraints in conjunction with the recent crystal structure of the cysteine desulfurase-ISCU-zinc complex and distance constraints from nuclear magnetic resonance. |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-モデル
モデル #1771 | タイプ: atomic / ダミー原子の半径: 1.90 A / カイ2乗値: 6.620329 / P-value: 0.000007 Omokage検索でこの集合体の類似形状データを探す (詳細) |
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モデル #1773 | タイプ: atomic / ダミー原子の半径: 1.90 A Omokage検索でこの集合体の類似形状データを探す (詳細) |
-試料
試料 | 名称: Human mitochondrial cysteine desulfurase-ISCU (NFS1, ISD11, Acp and ISCU heterodimer complex) 試料濃度: 2.00-10.00 / Entity id: 958 / 959 / 960 / 961 |
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バッファ | 名称: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP / pH: 7.5 / コメント: HNT Buffer |
要素 #958 | 名称: NFS1 / タイプ: protein / 記述: Cysteine desulfurase, mitochondrial / 分子量: 44.913 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: Q9Y697 配列: GPVLRPLYMD VQATTPLDPR VLDAMLPYLI NYYGNPHSRT HAYGWESEAA MERARQQVAS LIGADPREII FTSGATESNN IAIKGVARFY RSRKKHLITT QTEHKCVLDS CRSLEAEGFQ VTYLPVQKSG IIDLKELEAA IQPDTSLVSV MTVNNEIGVK QPIAEIGRIC ...配列: GPVLRPLYMD VQATTPLDPR VLDAMLPYLI NYYGNPHSRT HAYGWESEAA MERARQQVAS LIGADPREII FTSGATESNN IAIKGVARFY RSRKKHLITT QTEHKCVLDS CRSLEAEGFQ VTYLPVQKSG IIDLKELEAA IQPDTSLVSV MTVNNEIGVK QPIAEIGRIC SSRKVYFHTD AAQAVGKIPL DVNDMKIDLM SISGHKIYGP KGVGAIYIRR RPRVRVEALQ SGGGQERGMR SGTVPTPLVV GLGAACEVAQ QEMEYDHKRI SKLSERLIQN IMKSLPDVVM NGDPKHHYPG CINLSFAYVE GESLLMALKD VALSSGSACT SASLEPSYVL RAIGTDEDLA HSSIRFGIGR FTTEEEVDYT VEKCIQHVKR LREMSPLWEM VQDGIDLKSI KWTQH |
要素 #959 | 名称: ISD11 / タイプ: protein / 記述: LYR motif-containing protein 4 / 分子量: 11.581 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: Q9HD34 配列: MAASSRAQVL SLYRAMLRES KRFSAYNYRT YAVRRIRDAF RENKNVKDPV EIQTLVNKAK RDLGVIRRQV HIGQLYSTDK LIIENRDMPR THHHHHH |
要素 #960 | タイプ: protein / 記述: Acyl carrier protein / 分子量: 11.5 / 分子数: 2 / 由来: Escherichia coli (strain K12) / 参照: UniProt: P0A6A8 配列: PspP0A6A8A CPECOLIAcy lcarrierpr oteinOSEsc herichiaco li(strainK12)GNacpPP E1SV2MSTIE ERVKKIIGEQ LGVKQEEVTN NASFVEDLGA DSLDTVELVM ALEEEFDTEI PDEEAEKITT VQAAIDYING HQA |
要素 #961 | 名称: ISCU / タイプ: protein 記述: Iron-sulfur cluster assembly enzyme ISCU, mitochondrial 分子量: 14.655 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: Q9H1K1 配列: RLYHKKVVDH YENPRNVGSL DKTSKNVGTG LVGAPACGDV MKLQIQVDEK GKIVDARFKT FGCGSAIASS SLATEWVKGK TVEEALTIKN TDIAKELCLP PVKLHCSMLA EDAIKAALAD YKLKQEPKKG EAEKK |
-実験情報
ビーム | 設備名称: NMRFAM Bruker Nanostar / 地域: Madison, WI / 国: USA / 線源: X-ray in houseX線 / 波長: 0.15418 Å / スペクトロメータ・検出器間距離: 1 mm | |||||||||||||||||||||||||||||||||
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検出器 | 名称: VÅNTEC-2000 / タイプ: MikroGap / Pixsize x: 200 mm | |||||||||||||||||||||||||||||||||
スキャン | タイトル: Human mitochondrial cysteine desulfurase-ISCU (NFS1, ISD11, Acp and ISCU heterodimer complex) 測定日: 2017年5月22日 / 保管温度: 25 °C / セル温度: 25 °C / 照射時間: 3600 sec. / フレーム数: 4 / 単位: 1/A /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 4.5a / ポイント数: 130 /
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結果 | カーブのタイプ: single_conc コメント: The solution SAXS study of cysteine desulfurase complex containing the scaffold protein ISCU
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