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データを開く
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基本情報
登録情報 | データベース: SASBDB / ID: SASDDB3 |
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![]() | Human mitochondrial cysteine desulfurase (NFS1, ISD11 and Acp heterodimer complex)
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機能・相同性 | ![]() molybdopterin cofactor metabolic process / Molybdenum cofactor biosynthesis / [4Fe-4S] cluster assembly / iron incorporation into metallo-sulfur cluster / L-cysteine desulfurase complex / Mitochondrial iron-sulfur cluster biogenesis / mitochondrial [2Fe-2S] assembly complex / cysteine desulfurase / cysteine desulfurase activity / Mo-molybdopterin cofactor biosynthetic process ...molybdopterin cofactor metabolic process / Molybdenum cofactor biosynthesis / [4Fe-4S] cluster assembly / iron incorporation into metallo-sulfur cluster / L-cysteine desulfurase complex / Mitochondrial iron-sulfur cluster biogenesis / mitochondrial [2Fe-2S] assembly complex / cysteine desulfurase / cysteine desulfurase activity / Mo-molybdopterin cofactor biosynthetic process / iron-sulfur cluster assembly complex / [2Fe-2S] cluster assembly / lipid biosynthetic process / iron-sulfur cluster assembly / lipid A biosynthetic process / iron-sulfur cluster binding / phosphopantetheine binding / acyl binding / acyl carrier activity / fatty acid biosynthetic process / pyridoxal phosphate binding / nuclear body / mitochondrial matrix / response to xenobiotic stimulus / centrosome / lipid binding / protein homodimerization activity / mitochondrion / nucleoplasm / nucleus / metal ion binding / cytoplasm / cytosol 類似検索 - 分子機能 |
生物種 | ![]() ![]() ![]() |
![]() | ![]() タイトル: Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. 著者: Kai Cai / Ronnie O Frederick / Hesam Dashti / John L Markley / ![]() 要旨: Cysteine desulfurase plays a central role in mitochondrial iron-sulfur cluster biogenesis by generating sulfur through the conversion of L-cysteine to L-alanine and by serving as the platform for ...Cysteine desulfurase plays a central role in mitochondrial iron-sulfur cluster biogenesis by generating sulfur through the conversion of L-cysteine to L-alanine and by serving as the platform for assembling other components of the biosynthetic machinery, including ISCU, frataxin, and ferredoxin. The human mitochondrial cysteine desulfurase complex consists of two copies each of NFS1, ISD11, and acyl carrier protein. We describe results from chemical crosslinking coupled with tandem mass spectrometry and small-angle X-ray scattering studies that are consistent with a closed NFS1 dimer rather than an open one for both the cysteine desulfurase-ISCU and cysteine desulfurase-ISCU-frataxin complexes. We present a structural model for the cysteine desulfurase-ISCU-frataxin complex derived from chemical crosslinking restraints in conjunction with the recent crystal structure of the cysteine desulfurase-ISCU-zinc complex and distance constraints from nuclear magnetic resonance. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
-モデル
モデル #1774 | ![]() タイプ: atomic / ダミー原子の半径: 1.90 A / カイ2乗値: 0.925444 / P-value: 0.253126 ![]() |
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モデル #1775 | ![]() タイプ: atomic / ダミー原子の半径: 1.90 A / カイ2乗値: 2.617924 ![]() |
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試料
![]() | 名称: Human mitochondrial cysteine desulfurase (NFS1, ISD11 and Acp heterodimer complex) 試料濃度: 2.00-10.00 / Entity id: 958 / 959 / 960 |
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バッファ | 名称: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP / pH: 7.5 / コメント: HNT Buffer |
要素 #958 | 名称: NFS1 / タイプ: protein / 記述: Cysteine desulfurase, mitochondrial / 分子量: 44.913 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: Q9Y697 配列: GPVLRPLYMD VQATTPLDPR VLDAMLPYLI NYYGNPHSRT HAYGWESEAA MERARQQVAS LIGADPREII FTSGATESNN IAIKGVARFY RSRKKHLITT QTEHKCVLDS CRSLEAEGFQ VTYLPVQKSG IIDLKELEAA IQPDTSLVSV MTVNNEIGVK QPIAEIGRIC ...配列: GPVLRPLYMD VQATTPLDPR VLDAMLPYLI NYYGNPHSRT HAYGWESEAA MERARQQVAS LIGADPREII FTSGATESNN IAIKGVARFY RSRKKHLITT QTEHKCVLDS CRSLEAEGFQ VTYLPVQKSG IIDLKELEAA IQPDTSLVSV MTVNNEIGVK QPIAEIGRIC SSRKVYFHTD AAQAVGKIPL DVNDMKIDLM SISGHKIYGP KGVGAIYIRR RPRVRVEALQ SGGGQERGMR SGTVPTPLVV GLGAACEVAQ QEMEYDHKRI SKLSERLIQN IMKSLPDVVM NGDPKHHYPG CINLSFAYVE GESLLMALKD VALSSGSACT SASLEPSYVL RAIGTDEDLA HSSIRFGIGR FTTEEEVDYT VEKCIQHVKR LREMSPLWEM VQDGIDLKSI KWTQH |
要素 #959 | 名称: ISD11 / タイプ: protein / 記述: LYR motif-containing protein 4 / 分子量: 11.581 / 分子数: 2 / 由来: Homo sapiens / 参照: UniProt: Q9HD34 配列: MAASSRAQVL SLYRAMLRES KRFSAYNYRT YAVRRIRDAF RENKNVKDPV EIQTLVNKAK RDLGVIRRQV HIGQLYSTDK LIIENRDMPR THHHHHH |
要素 #960 | タイプ: protein / 記述: Acyl carrier protein / 分子量: 11.5 / 分子数: 2 / 由来: Escherichia coli (strain K12) / 参照: UniProt: P0A6A8 配列: PspP0A6A8A CPECOLIAcy lcarrierpr oteinOSEsc herichiaco li(strainK12)GNacpPP E1SV2MSTIE ERVKKIIGEQ LGVKQEEVTN NASFVEDLGA DSLDTVELVM ALEEEFDTEI PDEEAEKITT VQAAIDYING HQA |
-実験情報
ビーム | 設備名称: NMRFAM Bruker Nanostar / 地域: Madison, WI / 国: USA ![]() | ||||||||||||||||||||||||||||||||||||
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検出器 | 名称: VÅNTEC-2000 / タイプ: MikroGap / Pixsize x: 200 mm | ||||||||||||||||||||||||||||||||||||
スキャン | 測定日: 2017年5月22日 / 保管温度: 4 °C / セル温度: 25 °C / 照射時間: 3600 sec. / フレーム数: 4 / 単位: 1/A /
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距離分布関数 P(R) |
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結果 | コメント: Solution SAXS study the human mitochondrial cysteine desulfurase, which consists of three components: NFS1, ISD11 and Acp and forms a homodimer.
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