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- SASDD99: Structure of Halobacterium salinarum VNG0258H/RosR in the presenc... -

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Basic information

Entry
Database: SASBDB / ID: SASDD99
SampleStructure of Halobacterium salinarum VNG0258H/RosR in the presence of 2 M NaCl
  • VNG0258H/RosR (protein), VNG0258H/RosR, Halobacterium salinarum NRC-1
Function / homologyTranscription regulator PadR, N-terminal / Transcriptional regulator PadR-like family / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily / metal ion binding / PadR family transcription regulator RosR
Function and homology information
Biological speciesHalobacterium salinarum NRC-1 (Halophile)
CitationDate: 2018 Aug
Title: The 3-D structure of VNG0258H/RosR – a haloarchaeal DNA-binding protein in its ionic shell
Authors: Kutnowski N / Shmuely H / Dahan I / Shmulevich F / Davidov G / Shahar A / Eichler J / Zarivach R
Contact author
  • Nitzan kutnowski (Ben-Gurion University of the Negev)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #2135
Type: dummy / Radius of dummy atoms: 2.80 A / Chi-square value: 1.100401 / P-value: 0.031878
Search similar-shape structures of this assembly by Omokage search (details)
Model #2143
Type: dummy / Software: (5) / Radius of dummy atoms: 2.00 A / Chi-square value: 1.100401 / P-value: 0.031878
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Structure of Halobacterium salinarum VNG0258H/RosR in the presence of 2 M NaCl
Specimen concentration: 8 mg/ml
BufferName: 50 mM HEPES, 2 M NaCl, 0.02% NaN3 / pH: 7
Entity #1167Name: VNG0258H/RosR / Type: protein / Description: VNG0258H/RosR / Formula weight: 14.533 / Num. of mol.: 2 / Source: Halobacterium salinarum NRC-1 / References: UniProt: Q9HSF4
Sequence:
MSEAQPDARS DARDLTAFQK NILTVLGEEA RYGLAIKREL EEYYGEEVNH GRLYPNLDDL VNKGLVEKSE LDKRTNEYAL TNEGFDAVVD DLEWTLSKFV ADADRRERVE TIVADDAAAL EHHHHHH

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.09919 Å / Dist. spec. to detc.: 2.864 mm
DetectorName: Pilatus 1M / Pixsize x: 172 mm
Scan
Title: Structure of Halobacterium salinarum VNG0258H/RosR in the presence of 2 M NaCl
Measurement date: Mar 7, 2015 / Storage temperature: 25 °C / Cell temperature: 25 °C / Exposure time: 2 sec. / Number of frames: 10 / Unit: 1/nm /
MinMax
Q0.0337 4.9752
Distance distribution function P(R)
Sofotware P(R): GNOM 4.6 / Number of points: 276 /
MinMax
Q0.2684 2.874
P(R) point50 325
R0 7.7
Result
Type of curve: single_conc
Comments: Individual (top) and spatially averaged (DAMFILT, bottom) ab initio models are displayed.
ExperimentalPorod
MW32.07 kDa38.22 kDa
Volume-63.45 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I08.223 0.014 8.53 0.01
Radius of gyration, Rg2.23 nm0.007 2.41 nm0.27

MinMax
D-7.7
Guinier point26 83

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