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- SASDCU5: SAXS dilution series on ground Doryteuthis squid lens 100% layer -

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Basic information

Entry
Database: SASBDB / ID: SASDCU5
SampleSAXS dilution series on ground Doryteuthis squid lens 100% layer
  • S-crystallin, Q25367_DOROP (protein), S-crystallin, Q25367, Doryteuthis pealeii
Function / homology
Function and homology information


glutathione metabolic process
Similarity search - Function
S-crystallin / Glutathione S-transferase, C-terminal domain / Glutathione S-transferase, N-terminal domain / Glutathione transferase family / Glutathione S-transferase, C-terminal / Soluble glutathione S-transferase N-terminal domain profile. / Glutathione S-transferase, C-terminal-like / Soluble glutathione S-transferase C-terminal domain profile. / Glutathione S-transferase, N-terminal / Glutathione S-transferase, C-terminal domain superfamily / Thioredoxin-like superfamily
Similarity search - Domain/homology
Biological speciesDoryteuthis pealeii (longfin inshore squid)
CitationJournal: Science / Year: 2017
Title: Eye patches: Protein assembly of index-gradient squid lenses.
Authors: J Cai / J P Townsend / T C Dodson / P A Heiney / A M Sweeney /
Abstract: A parabolic relationship between lens radius and refractive index allows spherical lenses to avoid spherical aberration. We show that in squid, patchy colloidal physics resulted from an evolutionary ...A parabolic relationship between lens radius and refractive index allows spherical lenses to avoid spherical aberration. We show that in squid, patchy colloidal physics resulted from an evolutionary radiation of globular S-crystallin proteins. Small-angle x-ray scattering experiments on lens tissue show colloidal gels of S-crystallins at all radial positions. Sparse lens materials form via low-valence linkages between disordered loops protruding from the protein surface. The loops are polydisperse and bind via a set of hydrogen bonds between disordered side chains. Peripheral lens regions with low particle valence form stable, volume-spanning gels at low density, whereas central regions with higher average valence gel at higher densities. The proteins demonstrate an evolved set of linkers for self-assembly of nanoparticles into volumetric materials.
Contact author
  • Jing Cai (Penn, University of Pennsylvania, Philadelphia, PA, United States)

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Models

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Sample

SampleName: SAXS dilution series on ground Doryteuthis squid lens 100% layer
BufferName: PBS, phosphate buffered saline / pH: 7
Entity #672Name: S-crystallin, Q25367 / Type: protein / Description: S-crystallin, Q25367_DOROP / Formula weight: 27.289 / Num. of mol.: 2 / Source: Doryteuthis pealeii / References: UniProt: Q25367_DOR
Sequence: MPTYTLYYFN GRGRAEVLRM MFAAANVKYM DKRFEFNEWD KYRKDMPSMC VPVLEMDGGN KMPETMAIPR YLAREFGFYG KSNMDMMRCD YIADCFYEIM HDYMRYFHEK NGRFRSSMEG SGNGMNSPTG ASGDMNSNSN FDTYLQWRFM NTCNRVLPFL ERTLEMQNGG ...Sequence:
MPTYTLYYFN GRGRAEVLRM MFAAANVKYM DKRFEFNEWD KYRKDMPSMC VPVLEMDGGN KMPETMAIPR YLAREFGFYG KSNMDMMRCD YIADCFYEIM HDYMRYFHEK NGRFRSSMEG SGNGMNSPTG ASGDMNSNSN FDTYLQWRFM NTCNRVLPFL ERTLEMQNGG KAFFMGDQMM WCDMMCHCAL EGPFMENQSM LSKHPKLMAL RTRIASHPKI SGYLKSRSNT NW

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Experimental information

BeamInstrument name: National Synchrotron Light Source (NSLS) X9A
City: Brookhaven, NY / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.1 Å
DetectorName: Pilatus 300K / Type: 20Hz
Scan
Title: SAXS dilution series on ground Doryteuthis squid lens 100% layer
Measurement date: Nov 12, 2013 / Unit: 1/A /
MinMax
Q0.008 1.72
ResultExperimental MW: 55 kDa / I0 from Guinier: 794 / Rg from Guinier: 2.4 nm / Type of curve: single_conc

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