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Yorodumi- SASDCQ5: SAXS on intact Doryteuthis squid lens 100% layer (S-crystallin, Q... -
+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDCQ5 |
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Sample | SAXS on intact Doryteuthis squid lens 100% layer
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Function / homology | Function and homology information |
Biological species | Doryteuthis pealeii (longfin inshore squid) |
Citation | Journal: Science / Year: 2017 Title: Eye patches: Protein assembly of index-gradient squid lenses. Authors: J Cai / J P Townsend / T C Dodson / P A Heiney / A M Sweeney / Abstract: A parabolic relationship between lens radius and refractive index allows spherical lenses to avoid spherical aberration. We show that in squid, patchy colloidal physics resulted from an evolutionary ...A parabolic relationship between lens radius and refractive index allows spherical lenses to avoid spherical aberration. We show that in squid, patchy colloidal physics resulted from an evolutionary radiation of globular S-crystallin proteins. Small-angle x-ray scattering experiments on lens tissue show colloidal gels of S-crystallins at all radial positions. Sparse lens materials form via low-valence linkages between disordered loops protruding from the protein surface. The loops are polydisperse and bind via a set of hydrogen bonds between disordered side chains. Peripheral lens regions with low particle valence form stable, volume-spanning gels at low density, whereas central regions with higher average valence gel at higher densities. The proteins demonstrate an evolved set of linkers for self-assembly of nanoparticles into volumetric materials. |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Data source
SASBDB page | SASDCQ5 |
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-Related structure data
Related structure data | C: citing same article (ref.) |
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Similar structure data |
-External links
Related items in Molecule of the Month |
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-Models
Model #1259 | Type: dummy / Software: (1.1.2) / Radius of dummy atoms: 4.20 A / Symmetry: None / Chi-square value: 63.552784 Search similar-shape structures of this assembly by Omokage search (details) |
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-Sample
Sample | Name: SAXS on intact Doryteuthis squid lens 100% layer |
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Buffer | Name: Tissue fluid / pH: 7 |
Entity #672 | Name: S-crystallin, Q25367 / Type: protein / Description: S-crystallin, Q25367_DOROP / Formula weight: 27.289 / Num. of mol.: 2 / Source: Doryteuthis pealeii / References: UniProt: Q25367_DOR Sequence: MPTYTLYYFN GRGRAEVLRM MFAAANVKYM DKRFEFNEWD KYRKDMPSMC VPVLEMDGGN KMPETMAIPR YLAREFGFYG KSNMDMMRCD YIADCFYEIM HDYMRYFHEK NGRFRSSMEG SGNGMNSPTG ASGDMNSNSN FDTYLQWRFM NTCNRVLPFL ERTLEMQNGG ...Sequence: MPTYTLYYFN GRGRAEVLRM MFAAANVKYM DKRFEFNEWD KYRKDMPSMC VPVLEMDGGN KMPETMAIPR YLAREFGFYG KSNMDMMRCD YIADCFYEIM HDYMRYFHEK NGRFRSSMEG SGNGMNSPTG ASGDMNSNSN FDTYLQWRFM NTCNRVLPFL ERTLEMQNGG KAFFMGDQMM WCDMMCHCAL EGPFMENQSM LSKHPKLMAL RTRIASHPKI SGYLKSRSNT NW |
-Experimental information
Beam | Instrument name: National Synchrotron Light Source (NSLS) X9A City: Brookhaven, NY / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.103318 Å | ||||||
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Detector | Name: Pilatus 300K / Type: 20Hz | ||||||
Scan | Title: SAXS on intact Doryteuthis squid lens 100% layer / Measurement date: Jul 17, 2014 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 60 sec. / Number of frames: 1 / Unit: 1/A /
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Result | Experimental MW: 50 kDa / Rg from PR: 2.4 nm / Type of curve: single_conc /
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