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- SASDCL2: Tandem CBD from Clostridium histolyticum ColG collagenase at pCa 3 -

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Basic information

Entry
Database: SASBDB / ID: SASDCL2
SampleTandem CBD from Clostridium histolyticum ColG collagenase at pCa 3
  • Class1 collagenase (protein), tandem CBD, Hathewaya histolytica
Function / homology
Function and homology information


tripeptidase activity / microbial collagenase / collagen metabolic process / collagen binding / metalloendopeptidase activity / endopeptidase activity / serine-type endopeptidase activity / calcium ion binding / extracellular region / zinc ion binding
Similarity search - Function
Collagenase ColG, catalytic helper subdomain / Collagenase G catalytic helper subdomain / Peptidase M9A/M9B, collagenase, bacterial / Peptidase M9, collagenase, N-terminal domain / Collagenase / Peptidase family M9 N-terminal / Polycystic kidney disease (PKD) domain profile. / PKD domain / PKD domain superfamily / PKD/Chitinase domain ...Collagenase ColG, catalytic helper subdomain / Collagenase G catalytic helper subdomain / Peptidase M9A/M9B, collagenase, bacterial / Peptidase M9, collagenase, N-terminal domain / Collagenase / Peptidase family M9 N-terminal / Polycystic kidney disease (PKD) domain profile. / PKD domain / PKD domain superfamily / PKD/Chitinase domain / Repeats in polycystic kidney disease 1 (PKD1) and other proteins / Neutral zinc metallopeptidases, zinc-binding region signature. / Immunoglobulin-like fold
Similarity search - Domain/homology
Biological speciesHathewaya histolytica (bacteria)
CitationDate: 2017 Aug
Title: Structural Characterization of Bacterial Collagenases

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Models

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Sample

SampleName: Tandem CBD from Clostridium histolyticum ColG collagenase at pCa 3
Specimen concentration: 2.00-6.00
BufferName: 10 mM HEPES 100 mM NaCL, 2% glycerol / pH: 7.5 / Comment: 0.2 mM EGTA pCa=3
Entity #608Name: tandem CBD / Type: protein / Description: Class1 collagenase / Formula weight: 27.055 / Num. of mol.: 1 / Source: Hathewaya histolytica / References: UniProt: Q9S0X0_CL
Sequence: GSPGIPGTTT PITKEMEPND DIKEANGPIV EGVTVKGDLN GSDDADTFYF DVKEDGDVTI ELPYSGSSNF TWLVYKEGDD QNHIASGIDK NNSKVGTFKS TKGRHYVFIY KHDSASNISY SLNIKGLGNE KLKEKENNDS SDKATVIPNF NTTMQGSLLG DDSRDYYSFE ...Sequence:
GSPGIPGTTT PITKEMEPND DIKEANGPIV EGVTVKGDLN GSDDADTFYF DVKEDGDVTI ELPYSGSSNF TWLVYKEGDD QNHIASGIDK NNSKVGTFKS TKGRHYVFIY KHDSASNISY SLNIKGLGNE KLKEKENNDS SDKATVIPNF NTTMQGSLLG DDSRDYYSFE VKEEGEVNIE LDKKDEFGVT WTLHPESNIN DRITYGQVDG NKVSNKVKLR PGKYYLLVYK YSGSGNYELR VNK

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Experimental information

BeamInstrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS)
City: Berkeley, CA / 国: USA / Type of source: X-ray synchrotron / Wavelength: 0.1127 Å / Dist. spec. to detc.: 1.5 mm
DetectorName: Pilatus3 X 2M / Pixsize x: 172 mm
ScanTitle: Tandem CBD from Clostridium histolyticum ColG collagenase at pCa 3
Measurement date: Sep 14, 2015 / Storage temperature: 4 °C / Cell temperature: 10 °C / Exposure time: 0.5 sec. / Number of frames: 15 / Unit: 1/A /
MinMax
Q0.0084 0.5584
ResultType of curve: extrapolated /
ExperimentalPorod
MW27.1 kDa-
Volume-35.6 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I095.97 0.08 97.1 0.5
Radius of gyration, Rg2.43 nm0.03 2.4 nm0.3

MinMax
D-7.5
Guinier point38 89

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