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- SASDCK3: aldehyde dehydrogenase (Aldehyde-alcohol dehydrogenase, AdhE) -

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Open data


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Basic information

Entry
Database: SASBDB / ID: SASDCK3
Samplealdehyde dehydrogenase
  • Aldehyde-alcohol dehydrogenase (protein), AdhE, Escherichia coli O157:H7
Function / homology
Function and homology information


acetaldehyde dehydrogenase (acetylating) / acetaldehyde dehydrogenase (acetylating) activity / alcohol metabolic process / carbon utilization / alcohol dehydrogenase (NAD+) activity / alcohol dehydrogenase / metal ion binding
Similarity search - Function
Bifunctional aldehyde-alcohol dehydrogenase / Bifunctional aldehyde-alcohol dehydrogenase, C-terminal domain / Iron-type alcohol dehydrogenase-like / Iron-containing alcohol dehydrogenases signature 2. / Iron-containing alcohol dehydrogenases signature 1. / Alcohol dehydrogenase, iron-type, conserved site / Alcohol dehydrogenase, iron-type/glycerol dehydrogenase GldA / Iron-containing alcohol dehydrogenase / Aldehyde dehydrogenase domain / Aldehyde dehydrogenase family ...Bifunctional aldehyde-alcohol dehydrogenase / Bifunctional aldehyde-alcohol dehydrogenase, C-terminal domain / Iron-type alcohol dehydrogenase-like / Iron-containing alcohol dehydrogenases signature 2. / Iron-containing alcohol dehydrogenases signature 1. / Alcohol dehydrogenase, iron-type, conserved site / Alcohol dehydrogenase, iron-type/glycerol dehydrogenase GldA / Iron-containing alcohol dehydrogenase / Aldehyde dehydrogenase domain / Aldehyde dehydrogenase family / Aldehyde dehydrogenase, C-terminal / Aldehyde dehydrogenase, N-terminal / Aldehyde/histidinol dehydrogenase
Similarity search - Domain/homology
Bifunctional aldehyde-alcohol dehydrogenase AdhE
Similarity search - Component
Biological speciesEscherichia coli O157:H7 (bacteria)
Contact author
  • Liyana Azmi (University of Glasgow, Glasgow, Scotland, UK)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #1260
Type: dummy / Radius of dummy atoms: 2.40 A / Symmetry: P1 / Chi-square value: 1.774
Search similar-shape structures of this assembly by Omokage search (details)
Model #1261
Type: dummy / Software: (5.0 r9217) / Radius of dummy atoms: 2.50 A / Symmetry: P1 / Comment: this is the averaged representation of the model / Chi-square value: 1.774 / P-value: 0.459900
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: aldehyde dehydrogenase / Specimen concentration: 2 mg/ml
BufferName: 30 mM HEPES, 150 mM NaCl, 5% (v/v) glycerol / pH: 7.5
Entity #603Name: AdhE / Type: protein / Description: Aldehyde-alcohol dehydrogenase / Formula weight: 48.038 / Num. of mol.: 1 / Source: Escherichia coli O157:H7 / References: UniProt: P0A9Q8
Sequence: MAVTNVAELN ALVERVKKAQ REYASFTQEQ VDKIFRAAAL AAADARIPLA KMAVAESGMG IVEDKVIKNH FASEYIYNAY KDEKTCGVLS EDDTFGTITI AEPIGIICGI VPTTNPTSTA IFKSLISLKT RNAIIFSPHP RAKDATNKAA DIVLQAAIAA GAPKDLIGWI ...Sequence:
MAVTNVAELN ALVERVKKAQ REYASFTQEQ VDKIFRAAAL AAADARIPLA KMAVAESGMG IVEDKVIKNH FASEYIYNAY KDEKTCGVLS EDDTFGTITI AEPIGIICGI VPTTNPTSTA IFKSLISLKT RNAIIFSPHP RAKDATNKAA DIVLQAAIAA GAPKDLIGWI DQPSVELSNA LMHHPDINLI LATGGPGMVK AAYSSGKPAI GVGAGNTPVV IDETADIKRA VASVLMSKTF DNGVICASEQ SVVVVDSVYD AVRERFATHG GYLLQGKELK AVQDVILKNG ALNAAIVGQP AYKIAELAGF SVPENTKILI GEVTVVDESE PFAHEKLSPT LAMYRAKDFE DAVEKAEKLV AMGGIGHTSC LYTDQDNQPA RVSYFGQKMK TARILINTPA SQGGIGDLYN FKLAPSLTLG CGSWGGNSIS ENVGPKHLIN KKTVAHHHHH H

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Experimental information

BeamInstrument name: Diamond Light Source B21 / City: Oxfordshire / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 4.04 mm
DetectorName: Pilatus 2M
Scan
Title: Aldehyde dehydrogenase / Measurement date: Jan 30, 2017 / Storage temperature: 4 °C / Cell temperature: 4 °C / Exposure time: 1 sec. / Number of frames: 18 / Unit: 1/A /
MinMax
Q0.004 0.4415
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1264 /
MinMax
Q0.00859018 0.313717
P(R) point1 1264
R0 111
Result
Type of curve: single_conc
Comments: Aldehyde dehydrogenase (AldDH) is the N-terminal domain of the bifunctional alcohol/aldehyde dehydrogenase (AdhE; SASBDB entry SASDC72) spanning amino acids 1-445 of the full-length protein. ...Comments: Aldehyde dehydrogenase (AldDH) is the N-terminal domain of the bifunctional alcohol/aldehyde dehydrogenase (AdhE; SASBDB entry SASDC72) spanning amino acids 1-445 of the full-length protein. SAXS data were measured from a protein construct that included a C-terminal 6 x histidine affinity chromatography tag. The buffer subtraction was performed using Scatter. Subsequent analysis was performed using PRIMUS (ATSAS 2.7.1).
ExperimentalPorod
MW46.5 kDa46.5 kDa
Volume-74 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I00.1826 0.0002068 0.18 0.00016
Radius of gyration, Rg2.74 nm0.062 2.68 nm1.68

MinMax
D-11.1
Guinier point20 182

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