SASDC42: Ethylene Receptor 1 Cytosolic Domain (Ethylene Receptor 1, ETR1(-ΔTM))

基本情報

登録情報

データベース: SASBDB / ID: SASDC42

試料

Ethylene Receptor 1 Cytosolic Domain

• Ethylene Receptor 1 (protein), ETR1(-ΔTM), Arabidopsis thaliana

機能・相同性

分子機能:

ethylene receptor activity / regulation of seedling development / detection of ethylene stimulus / ethylene binding / seed dormancy process / defense response by callose deposition in cell wall / negative regulation of ethylene-activated signaling pathway / sugar mediated signaling pathway / response to gibberellin / response to insect / phloem or xylem histogenesis / cytokinin metabolic process / response to ethylene / regulation of stomatal movement / response to auxin / protein histidine kinase activity / response to abscisic acid / hydrogen peroxide biosynthetic process / histidine kinase / phosphorelay sensor kinase activity / response to salt stress / response to molecule of bacterial origin / defense response / response to heat / defense response to bacterium / cell division / endoplasmic reticulum membrane / endoplasmic reticulum / ATP binding / identical protein binding / metal ion binding

ドメイン・相同性:

Ethylene receptor / His Kinase A (phospho-acceptor) domain / His Kinase A (phosphoacceptor) domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily / Signal transduction histidine kinase-related protein, C-terminal / Histidine kinase domain / Histidine kinase domain profile. / Response regulator receiver domain / cheY-homologous receiver domain / Signal transduction response regulator, receiver domain / Response regulatory domain profile. / GAF domain / CheY-like superfamily / Domain present in phytochromes and cGMP-specific phosphodiesterases. / GAF domain / GAF-like domain superfamily / Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase / Histidine kinase-like ATPases / Histidine kinase/HSP90-like ATPase / Histidine kinase/HSP90-like ATPase superfamily

構成要素:

Ethylene receptor 1

• 生物種: Arabidopsis thaliana (シロイヌナズナ)
• 引用: ジャーナル: J Biol Chem / 年: 2015; タイトル: Structural model of the cytosolic domain of the plant ethylene receptor 1 (ETR1).; 著者: Hubert Mayerhofer / Saravanan Panneerselvam / Heidi Kaljunen / Anne Tuukkanen / Haydyn D T Mertens / Jochen Mueller-Dieckmann / ; 要旨: Ethylene initiates important aspects of plant growth and development through disulfide-linked receptor dimers located in the endoplasmic reticulum. The receptors feature a small transmembrane, ethylene binding domain followed by a large cytosolic domain, which serves as a scaffold for the assembly of large molecular weight complexes of different ethylene receptors and other cellular participants of the ethylene signaling pathway. Here we report the crystallographic structures of the ethylene receptor 1 (ETR1) catalytic ATP-binding and the ethylene response sensor 1 dimerization histidine phosphotransfer (DHp) domains and the solution structure of the entire cytosolic domain of ETR1, all from Arabidopsis thaliana. The isolated dimeric ethylene response sensor 1 DHp domain is asymmetric, the result of different helical bending angles close to the conserved His residue. The structures of the catalytic ATP-binding, DHp, and receiver domains of ethylene receptors and of a homologous, but dissimilar, GAF domain were refined against experimental small angle x-ray scattering data, leading to a structural model of the entire cytosolic domain of the ethylene receptor 1. The model illustrates that the cytosolic domain is shaped like a dumbbell and that the receiver domain is flexible and assumes a position different from those observed in prokaryotic histidine kinases. Furthermore the cytosolic domain of ETR1 plays a key role, interacting with all other receptors and several participants of the ethylene signaling pathway. Our model, therefore, provides the first step toward a detailed understanding of the molecular mechanics of this important signal transduction process in plants.

登録者

• Anne Tuukkanen
• Haydyn Mertens

モデル

試料

• 試料: 名称: Ethylene Receptor 1 Cytosolic Domain / Dry vol: 78367 / 試料濃度: 0.70-4.20 / 濃度測定法: A280
• バッファ: 名称: 20 mM TRIS 150 mM NaCl 1mM DTT 250mM NDSB / 濃度: 20.00 mM / pH: 8.8 / 組成: 150 mM NaCl, 1mM DTT, 250mM NDSB

要素 #515

名称: ETR1(-ΔTM) / タイプ: protein / 記述: Ethylene Receptor 1 / 分子量: 64.555 / 分子数: 2 / 由来: Arabidopsis thaliana / 参照: UniProt: P49333
配列:

DRHTILKTTL VELGRTLALE ECALWMPTRT GLELQLSYTL RHQHPVEYTV PIQLPVINQV FGTSRAVKIS PNSPVARLRP VSGKYMLGEV VAVRVPLLHL SNFQINDWPE LSTKRYALMV LMLPSDSARQ WHVHELELVE VVADQVAVAL SHAAILEESM RARDLLMEQN VALDLARREA ETAIRARNDF LAVMNHEMRT PMHAIIALSS LLQETELTPE QRLMVETILK SSNLLATLMN DVLDLSRLED GSLQLELGTF NLHTLFREVL NLIKPIAVVK KLPITLNLAP DLPEFVVGDE KRLMQIILNI VGNAVKFSKQ GSISVTALVT KSDTRAADFF VVPTGSHFYL RVKVKDSGAG INPQDIPKIF TKFAQTQSLA TRSSGGSGLG LAISKRFVNL MEGNIWIESD GLGKGCTAIF DVKLGISERS NESKQSGIPK VPAIPRHSNF TGLKVLVMDE NGVSRMVTKG LLVHLGCEVT TVSSNEECLR VVSHEHKVVF MDVCMPGVEN YQIALRIHEK FTKQRHQRPL LVALSGNTDK STKEKCMSFG LDGVLLKPVS LDNIRDVLSD LLEPRVLYEG M

実験情報

• ビーム: 設備名称: PETRA III P12 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron / 波長: 1.24 Å / スペクトロメータ・検出器間距離: 3.1 mm
• 検出器: 名称: Pilatus 2M

スキャン

タイトル: ETR1-delTM / 測定日: 2013年3月29日 / 保管温度: 10 °C / セル温度: 10 °C / 照射時間: 0.05 sec. / フレーム数: 20 / 単位: 1/nm /

	Min	Max
Q	0.0758	4.6653

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 482 /

	Min	Max
Q	0.123649	1.40266
P(R) point	1	482
R	0	19

結果

カーブのタイプ: merged / Standard: BSA / コメント: ETR1 in the absence of NDSB

	実験値	Experimental error	Standard	Standard error	Porod	Porod error
分子量	53 kDa	5	53 kDa	5	161.2 kDa	24
体積	-	-	-	-	274 nm3	42

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	1164	40	1124	25
慣性半径, Rg	5.9 nm	0.6	5.5 nm	1.1

	Min	Max	Error
D	-	19	0.5
Guinier point	19	42	-