+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDBX3 |
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Sample | Aureobox dsDNA
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Biological species | synthetic construct (others) |
Citation | Journal: Nucleic Acids Res / Year: 2016 Title: Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes. Authors: Ankan Banerjee / Elena Herman / Manuel Serif / Manuel Maestre-Reyna / Sebastian Hepp / Richard Pokorny / Peter G Kroth / Lars-Oliver Essen / Tilman Kottke / Abstract: The modular architecture of aureochrome blue light receptors, found in several algal groups including diatoms, is unique by having the LOV-type photoreceptor domain fused to the C-terminus of its ...The modular architecture of aureochrome blue light receptors, found in several algal groups including diatoms, is unique by having the LOV-type photoreceptor domain fused to the C-terminus of its putative effector, an N-terminal DNA-binding bZIP module. The structural and functional understanding of aureochromes' light-dependent signaling mechanism is limited, despite their promise as an optogenetic tool. We show that class I aureochromes 1a and 1c from the diatom Phaeodactylum tricornutum are regulated in a light-independent circadian rhythm. These aureochromes are capable to form functional homo- and heterodimers, which recognize the ACGT core sequence within the canonical 'aureo box', TGACGT, in a light-independent manner. The bZIP domain holds a more folded and less flexible but extended conformation in the duplex DNA-bound state. FT-IR spectroscopy in the absence and the presence of DNA shows light-dependent helix unfolding in the LOV domain, which leads to conformational changes in the bZIP region. The solution structure of DNA bound to aureochrome points to a tilted orientation that was further validated by molecular dynamics simulations. We propose that aureochrome signaling relies on an allosteric pathway from LOV to bZIP that results in conformational changes near the bZIP-DNA interface without major effects on the binding affinity. |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Models
Model #458 | Type: dummy / Software: DAMFILT / Radius of dummy atoms: 2.00 A / Comment: Averaged and filtered/volume corrected model / Chi-square value: 0.677 / P-value: 0.446000 Search similar-shape structures of this assembly by Omokage search (details) |
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-Sample
Sample | Name: Aureobox dsDNA / Specimen concentration: 1.25 mg/ml |
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Buffer | Name: Tris / Concentration: 50.00 mM / pH: 8 / Composition: 50 mM boric acid, 1 mM EDTA |
Entity #311 | Type: DNA / Description: Aureobox dsDNA / Formula weight: 12.993 / Num. of mol.: 1 / Source: synthetic construct Sequence: GAATTGTTGA CGTGGAGTTG CGCAACTCCA CGTCAACAAT TC |
-Experimental information
Beam | Instrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.93 Å / Dist. spec. to detc.: 2.43 mm | ||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M | ||||||||||||||||||||||||||||||
Scan | Title: Aureobox dsDNA / Measurement date: Nov 6, 2014 / Storage temperature: 4 °C / Cell temperature: 4 °C / Exposure time: 20 sec. / Number of frames: 10 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 617 /
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Result | Type of curve: single_conc
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