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-基本情報
登録情報 | データベース: SASBDB / ID: SASDBL3 |
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試料 | Highly similar to Actin cross-linking family protein 7 (ACF7) Homo Sapiens
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機能・相同性 | 機能・相同性情報 intermediate filament cytoskeleton organization / cytoskeletal protein binding / cytoskeleton 類似検索 - 分子機能 |
生物種 | Homo sapiens (ヒト) |
引用 | ジャーナル: Nat Commun / 年: 2016 タイトル: In vivo epidermal migration requires focal adhesion targeting of ACF7. 著者: Jiping Yue / Yao Zhang / Wenguang G Liang / Xuewen Gou / Philbert Lee / Han Liu / Wanqing Lyu / Wei-Jen Tang / Shao-Yu Chen / Feng Yang / Hong Liang / Xiaoyang Wu / 要旨: Turnover of focal adhesions allows cell retraction, which is essential for cell migration. The mammalian spectraplakin protein, ACF7 (Actin-Crosslinking Factor 7), promotes focal adhesion dynamics by ...Turnover of focal adhesions allows cell retraction, which is essential for cell migration. The mammalian spectraplakin protein, ACF7 (Actin-Crosslinking Factor 7), promotes focal adhesion dynamics by targeting of microtubule plus ends towards focal adhesions. However, it remains unclear how the activity of ACF7 is regulated spatiotemporally to achieve focal adhesion-specific guidance of microtubule. To explore the potential mechanisms, we resolve the crystal structure of ACF7's NT (amino-terminal) domain, which mediates F-actin interactions. Structural analysis leads to identification of a key tyrosine residue at the calponin homology (CH) domain of ACF7, whose phosphorylation by Src/FAK (focal adhesion kinase) complex is essential for F-actin binding of ACF7. Using skin epidermis as a model system, we further demonstrate that the phosphorylation of ACF7 plays an indispensable role in focal adhesion dynamics and epidermal migration in vitro and in vivo. Together, our findings provide critical insights into the molecular mechanisms underlying coordinated cytoskeletal dynamics during cell movement. |
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-構造の表示
-ダウンロードとリンク
-モデル
-試料
試料 | 名称: Highly similar to Actin cross-linking family protein 7 (ACF7) Homo Sapiens 試料濃度: 1.02 mg/ml |
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バッファ | 名称: PBS / pH: 7.4 |
要素 #300 | 名称: ACF7 / タイプ: protein 記述: cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 分子量: 46.342 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: Q6ZSD7 配列: MFPVLWAGIP GRDVGSLQPL PPGFKQFCTS ASRVAVIADE RDRVQKKTFT KWVNKHLMKV RKHINDLYED LRDGHNLISL LEVLSGIKLP REKGRMRFHR LQNVQIALDF LKQRQVKLVN IRNDDITDGN PKLTLGLIWT IILHFQISDI YISGESGDMS AKEKLLLWTQ ...配列: MFPVLWAGIP GRDVGSLQPL PPGFKQFCTS ASRVAVIADE RDRVQKKTFT KWVNKHLMKV RKHINDLYED LRDGHNLISL LEVLSGIKLP REKGRMRFHR LQNVQIALDF LKQRQVKLVN IRNDDITDGN PKLTLGLIWT IILHFQISDI YISGESGDMS AKEKLLLWTQ KVTAGYTGIK CTNFSSCWSD GKMFNALIHR YRPDLVDMER VQIQSNRENL EQAFEVAERL GVTRLLDAED VDVPSPDEKS VITYVSSIYD AFPKVPEGGE GISATEVDSR WQEYQSRVDS LIPWIKQHTI LMSDKTFPQN PVELKALYNQ YIHFKETEIL AKEREKGRIE ELYKLLEVWI EFGRIKLPQG YHPNDVEEEW GKLIIEMLER EKSLRPAVER LELLLQIANK |
-実験情報
ビーム | 設備名称: Advanced Photon Source (APS) 12ID-B SAXS/WAXS / 地域: Argonne, IL / 国: USA / 線源: X-ray synchrotron | |||||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 2M | |||||||||||||||||||||||||||||||||
スキャン | タイトル: Actin cross-linking family protein7 (ACF7) / 測定日: 2014年11月10日 / セル温度: 25 °C / 照射時間: 1 sec. / フレーム数: 12 / 単位: 1/nm /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 5.0 / ポイント数: 107 /
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結果 | カーブのタイプ: single_conc /
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