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Yorodumi- SASDBL3: Highly similar to Actin cross-linking family protein 7 (ACF7) Hom... -
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-Basic information
Entry | Database: SASBDB / ID: SASDBL3 |
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Sample | Highly similar to Actin cross-linking family protein 7 (ACF7) Homo Sapiens
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Function / homology | Function and homology information intermediate filament cytoskeleton organization / cytoskeletal protein binding / cytoskeleton Similarity search - Function |
Biological species | Homo sapiens (human) |
Citation | Journal: Nat Commun / Year: 2016 Title: In vivo epidermal migration requires focal adhesion targeting of ACF7. Authors: Jiping Yue / Yao Zhang / Wenguang G Liang / Xuewen Gou / Philbert Lee / Han Liu / Wanqing Lyu / Wei-Jen Tang / Shao-Yu Chen / Feng Yang / Hong Liang / Xiaoyang Wu / Abstract: Turnover of focal adhesions allows cell retraction, which is essential for cell migration. The mammalian spectraplakin protein, ACF7 (Actin-Crosslinking Factor 7), promotes focal adhesion dynamics by ...Turnover of focal adhesions allows cell retraction, which is essential for cell migration. The mammalian spectraplakin protein, ACF7 (Actin-Crosslinking Factor 7), promotes focal adhesion dynamics by targeting of microtubule plus ends towards focal adhesions. However, it remains unclear how the activity of ACF7 is regulated spatiotemporally to achieve focal adhesion-specific guidance of microtubule. To explore the potential mechanisms, we resolve the crystal structure of ACF7's NT (amino-terminal) domain, which mediates F-actin interactions. Structural analysis leads to identification of a key tyrosine residue at the calponin homology (CH) domain of ACF7, whose phosphorylation by Src/FAK (focal adhesion kinase) complex is essential for F-actin binding of ACF7. Using skin epidermis as a model system, we further demonstrate that the phosphorylation of ACF7 plays an indispensable role in focal adhesion dynamics and epidermal migration in vitro and in vivo. Together, our findings provide critical insights into the molecular mechanisms underlying coordinated cytoskeletal dynamics during cell movement. |
Contact author |
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-Structure visualization
-Downloads & links
-Data source
SASBDB page | SASDBL3 |
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-Related structure data
Related structure data | 4z6gC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Models
-Sample
Sample | Name: Highly similar to Actin cross-linking family protein 7 (ACF7) Homo Sapiens Specimen concentration: 1.02 mg/ml |
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Buffer | Name: PBS / pH: 7.4 |
Entity #300 | Name: ACF7 / Type: protein Description: cDNA FLJ45612 fis, clone BRTHA3025073, highly similar to Actin cross-linking family protein 7 Formula weight: 46.342 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q6ZSD7 Sequence: MFPVLWAGIP GRDVGSLQPL PPGFKQFCTS ASRVAVIADE RDRVQKKTFT KWVNKHLMKV RKHINDLYED LRDGHNLISL LEVLSGIKLP REKGRMRFHR LQNVQIALDF LKQRQVKLVN IRNDDITDGN PKLTLGLIWT IILHFQISDI YISGESGDMS AKEKLLLWTQ ...Sequence: MFPVLWAGIP GRDVGSLQPL PPGFKQFCTS ASRVAVIADE RDRVQKKTFT KWVNKHLMKV RKHINDLYED LRDGHNLISL LEVLSGIKLP REKGRMRFHR LQNVQIALDF LKQRQVKLVN IRNDDITDGN PKLTLGLIWT IILHFQISDI YISGESGDMS AKEKLLLWTQ KVTAGYTGIK CTNFSSCWSD GKMFNALIHR YRPDLVDMER VQIQSNRENL EQAFEVAERL GVTRLLDAED VDVPSPDEKS VITYVSSIYD AFPKVPEGGE GISATEVDSR WQEYQSRVDS LIPWIKQHTI LMSDKTFPQN PVELKALYNQ YIHFKETEIL AKEREKGRIE ELYKLLEVWI EFGRIKLPQG YHPNDVEEEW GKLIIEMLER EKSLRPAVER LELLLQIANK |
-Experimental information
Beam | Instrument name: Advanced Photon Source (APS) 12ID-B SAXS/WAXS City: Argonne, IL / 国: USA / Type of source: X-ray synchrotron | |||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 2M | |||||||||||||||||||||||||||||||||
Scan | Title: Actin cross-linking family protein7 (ACF7) / Measurement date: Nov 10, 2014 / Cell temperature: 25 °C / Exposure time: 1 sec. / Number of frames: 12 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 5.0 / Number of points: 107 /
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Result | Type of curve: single_conc /
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