SASDBD7: Human Proliferating Cell Nuclear Antigen (PCNA) (Proliferating ce...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDBD7

試料

Human Proliferating Cell Nuclear Antigen (PCNA)

• Proliferating cell nuclear antigen増殖細胞核抗原 (protein), Homo sapiens

機能・相同性

分子機能:

positive regulation of deoxyribonuclease activity / dinucleotide insertion or deletion binding / PCNA-p21 complex / mitotic telomere maintenance via semi-conservative replication / purine-specific mismatch base pair DNA N-glycosylase activity / positive regulation of DNA-directed DNA polymerase activity / 核ラミナ / MutLalpha complex binding / Polymerase switching / Telomere C-strand (Lagging Strand) Synthesis / Processive synthesis on the lagging strand / PCNA complex / Removal of the Flap Intermediate / Processive synthesis on the C-strand of the telomere / Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta) / Polymerase switching on the C-strand of the telomere / Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha) / Transcription of E2F targets under negative control by DREAM complex / Removal of the Flap Intermediate from the C-strand / replisome / response to L-glutamate / histone acetyltransferase binding / DNA polymerase processivity factor activity / G1/S-Specific Transcription / leading strand elongation / replication fork processing / response to dexamethasone / nuclear replication fork / SUMOylation of DNA replication proteins / estrous cycle / PCNA-Dependent Long Patch Base Excision Repair / cyclin-dependent protein kinase holoenzyme complex / DNAミスマッチ修復 / DNA修復 / response to cadmium ion / DNA polymerase binding / epithelial cell differentiation / positive regulation of DNA repair / Translesion synthesis by REV1 / base-excision repair, gap-filling / Translesion synthesis by POLK / Translesion synthesis by POLI / Gap-filling DNA repair synthesis and ligation in GG-NER / TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest / DNA複製 / positive regulation of DNA replication / male germ cell nucleus / liver regeneration / nuclear estrogen receptor binding / Recognition of DNA damage by PCNA-containing replication complex / Termination of translesion DNA synthesis / Translesion Synthesis by POLH / HDR through Homologous Recombination (HRR) / Dual Incision in GG-NER / receptor tyrosine kinase binding / cellular response to hydrogen peroxide / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / cellular response to UV / cellular response to xenobiotic stimulus / E3 ubiquitin ligases ubiquitinate target proteins / response to estradiol / heart development / damaged DNA binding / chromosome, telomeric region / nuclear body / 中心体 / chromatin binding / クロマチン / protein-containing complex binding / enzyme binding / negative regulation of transcription by RNA polymerase II / extracellular exosome / 核質 / identical protein binding / 細胞核

ドメイン・相同性:

Proliferating cell nuclear antigen signature 2. / Proliferating cell nuclear antigen, PCNA, conserved site / Proliferating cell nuclear antigen signature 1. / Proliferating cell nuclear antigen, PCNA / Proliferating cell nuclear antigen, PCNA, N-terminal / Proliferating cell nuclear antigen, PCNA, C-terminal / Proliferating cell nuclear antigen, N-terminal domain / Proliferating cell nuclear antigen, C-terminal domain / :

構成要素:

増殖細胞核抗原

• 生物種: Homo sapiens (ヒト)
• 引用: ジャーナル: Nucleic Acids Res / 年: 2017; タイトル: Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase.; 著者: Aishwarya Prakash / Kedar Moharana / Susan S Wallace / Sylvie Doublié / ; 要旨: The base excision repair (BER) pathway repairs oxidized lesions in the DNA that result from reactive oxygen species generated in cells. If left unrepaired, these damaged DNA bases can disrupt cellular processes such as replication. NEIL1 is one of the 11 human DNA glycosylases that catalyze the first step of the BER pathway, i.e. recognition and excision of DNA lesions. NEIL1 interacts with essential replication proteins such as the ring-shaped homotrimeric proliferating cellular nuclear antigen (PCNA). We isolated a complex formed between NEIL1 and PCNA (±DNA) using size exclusion chromatography (SEC). This interaction was confirmed using native gel electrophoresis and mass spectrometry. Stokes radii measured by SEC hinted that PCNA in complex with NEIL1 (±DNA) was no longer a trimer. Height measurements and images obtained by atomic force microscopy also demonstrated the dissociation of the PCNA homotrimer in the presence of NEIL1 and DNA, while small-angle X-ray scattering analysis confirmed the NEIL1 mediated PCNA trimer dissociation and formation of a 1:1:1 NEIL1-DNA-PCNA(monomer) complex. Furthermore, ab initio shape reconstruction provides insights into the solution structure of this previously unreported complex. Together, these data point to a potential mechanistic switch between replication and BER.

登録者

• Kedar Moharana (University of Vermont, Burlington, VT, USA)

モデル

• モデル #808: ; タイプ: dummy / ソフトウェア: GASBOR / ダミー原子の半径: 1.90 A / カイ2乗値: 0.75; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Human Proliferating Cell Nuclear Antigen (PCNA) / 試料濃度: 1.00-6.00
• バッファ: 名称: 25mM HEPES 100mM NaCl 1mM DTT / pH: 7.5

要素 #452

タイプ: protein
記述: Proliferating cell nuclear antigen増殖細胞核抗原
分子量: 29.669 / 分子数: 3 / 由来: Homo sapiens / 参照: UniProt: P12004
配列:

MHHHHHHMFE ARLVQGSILK KVLEALKDLI NEACWDISSS GVNLQSMDSS HVSLVQLTLR SEGFDTYRCD RNLAMGVNLT SMSKILKCAG NEDIITLRAE DNADTLALVF EAPNQEKVSD YEMKLMDLDV EQLGIPEQEY SCVVKMPSGE FACICRDLSH IGDAVVISCA KDGVKFSASG ELGNGNIKLS QTSNVDKEEE AVTIEMNEPV QLTFALRYLN FFTKATPLSS TVTLSMSADV PLVVEYKIAD MGHLKYYLAP KIEDEEGS

実験情報

• ビーム: 設備名称: Advanced Light Source (ALS) 12.3.1 (SIBYLS) / 地域: Berkeley, CA / 国: USA / 線源: X-ray synchrotronシンクロトロン / 波長: 0.1127 Å / スペクトロメータ・検出器間距離: 1.4 mm
• 検出器: 名称: Pilatus3 X 2M / Pixsize x: 172 mm

スキャン

タイトル: Human Proliferating Cell Nuclear Antigen (PCNA)
測定日: 2016年1月20日 / 保管温度: 4 °C / セル温度: 10 °C / 照射時間: 0.2 sec. / フレーム数: 24 / 単位: 1/A /

	Min	Max
Q	0.0164	0.5285

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 919 /

	Min	Max
Q	0.017002	0.528544
P(R) point	1	919
R	0	97.2

結果

カーブのタイプ: single_conc
コメント: Experimental MW reported here is from SAXSMoW. PCNA was used to calibrate forward intensity for MW estimation of other scatterers.

	実験値	Porod
分子量	85.9 kDa	75.414 kDa
体積	-	128.2 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	486.2	1.008	483.2	1.4
慣性半径, Rg	3.439 nm	0.052	3.44 nm	0.025

	Min	Max	Error
D	-	9.72	0.18
Guinier point	2	39	-