+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDB44 |
---|---|
試料 | Aureochrome 1a from P. tricornutum, amino acids 148-378 (N-terminal truncation), dark state
|
生物種 | Phaeodactylum tricornutum (海洋性珪藻) |
引用 | ジャーナル: Elife / 年: 2016 タイトル: Blue light-induced LOV domain dimerization enhances the affinity of Aureochrome 1a for its target DNA sequence. 著者: Udo Heintz / Ilme Schlichting / 要旨: The design of synthetic optogenetic tools that allow precise spatiotemporal control of biological processes previously inaccessible to optogenetic control has developed rapidly over the last years. ...The design of synthetic optogenetic tools that allow precise spatiotemporal control of biological processes previously inaccessible to optogenetic control has developed rapidly over the last years. Rational design of such tools requires detailed knowledge of allosteric light signaling in natural photoreceptors. To understand allosteric communication between sensor and effector domains, characterization of all relevant signaling states is required. Here, we describe the mechanism of light-dependent DNA binding of the light-oxygen-voltage (LOV) transcription factor Aureochrome 1a from Phaeodactylum tricornutum (PtAu1a) and present crystal structures of a dark state LOV monomer and a fully light-adapted LOV dimer. In combination with hydrogen/deuterium-exchange, solution scattering data and DNA-binding experiments, our studies reveal a light-sensitive interaction between the LOV and basic region leucine zipper DNA-binding domain that together with LOV dimerization results in modulation of the DNA affinity of PtAu1a. We discuss the implications of these results for the design of synthetic LOV-based photosensors with application in optogenetics. |
登録者 |
|
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
---|
-ダウンロードとリンク
-モデル
モデル #481 | タイプ: dummy / ソフトウェア: DAMSTART/DAMMIN / ダミー原子の半径: 2.50 A / 対称性: P1 / カイ2乗値: 8.567329 Omokage検索でこの集合体の類似形状データを探す (詳細) |
---|
-試料
試料 | 名称: Aureochrome 1a from P. tricornutum, amino acids 148-378 (N-terminal truncation), dark state |
---|---|
バッファ | 名称: HEPES / 濃度: 20.00 mM / pH: 7.5 / 組成: 100 mM NaCl, 10 mM MgCl2, 5% w/v glycerol |
要素 #313 | タイプ: protein / 記述: Aureochrome 1a (N-terminally truncated) / 分子量: 26.277 / 分子数: 2 / 由来: Phaeodactylum tricornutum 配列: GAMGMSEQQK VERRERNREH AKRSRIRKKF LLESLQQSVS LLKEENEKLK TSIRSHLGEE KADTLIDSAN NNKTDVDGLL ASSQGIANKV LDDPDFSFIK ALQTAQQNFV VTDPSLPDNP IVYASQGFLN LTGYSLDQIL GRNCRFLQGP ETDPKAVERI RKAIEQGNDM ...配列: GAMGMSEQQK VERRERNREH AKRSRIRKKF LLESLQQSVS LLKEENEKLK TSIRSHLGEE KADTLIDSAN NNKTDVDGLL ASSQGIANKV LDDPDFSFIK ALQTAQQNFV VTDPSLPDNP IVYASQGFLN LTGYSLDQIL GRNCRFLQGP ETDPKAVERI RKAIEQGNDM SVCLLNYRVD GTTFWNQFFI AALRDAGGNV TNFVGVQCKV SDQYAATVTK QQEEEEEAAA NDDED |
-実験情報
ビーム | 設備名称: Swiss Light Source cSAXS / 地域: Villigen / 国: Switzerland / 線源: X-ray synchrotron / 波長: 0.1 Å / スペクトロメータ・検出器間距離: 2.15 mm | |||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
検出器 | 名称: Pilatus 2M | |||||||||||||||||||||||||||||||||||||||
スキャン | タイトル: N-terminally truncated Aureochrome 1a / 測定日: 2015年3月11日 / 保管温度: 10 °C / セル温度: 10 °C / 照射時間: 0.5 sec. / フレーム数: 10 / 単位: 1/nm /
| |||||||||||||||||||||||||||||||||||||||
距離分布関数 P(R) | ソフトウェア P(R): GNOM 4.5a / ポイント数: 282 /
| |||||||||||||||||||||||||||||||||||||||
結果 | カーブのタイプ: single_conc
|