+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDAX5 |
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試料 | Endophilin-CoA complex
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生物種 | Mus musculus (ハツカネズミ) |
引用 | ジャーナル: Front Mol Biosci / 年: 2014 タイトル: Endophilin-A1 BAR domain interaction with arachidonyl CoA. 著者: Maxim V Petoukhov / Winfried Weissenhorn / Dmitri I Svergun / 要旨: Endophilin-A1 belongs to the family of BAR domain containing proteins that catalyze membrane remodeling processes via sensing, inducing and stabilizing membrane curvature. We show that the BAR domain ...Endophilin-A1 belongs to the family of BAR domain containing proteins that catalyze membrane remodeling processes via sensing, inducing and stabilizing membrane curvature. We show that the BAR domain of endophilin-A1 binds arachidonic acid and molds its coenzyme A (CoA) activated form, arachidonyl-CoA into a defined structure. We studied low resolution structures of endophilin-A1-BAR and its complex with arachidonyl-CoA in solution using synchrotron small-angle X-ray scattering (SAXS). The free endophilin-A1-BAR domain is shown to be dimeric at lower concentrations but builds tetramers and higher order complexes with increasing concentrations. Extensive titration SAXS studies revealed that the BAR domain produces a homogenous complex with the lipid micelles. The structural model of the complexes revealed two arachidonyl-CoA micelles bound to the distal arms of an endophilin-A1-BAR dimer. Intriguingly, the radius of the bound micelles significantly decreases compared to that of the free micelles, and this structural result may provide hints on the potential biological relevance of the endophilin-A1-BAR interaction with arachidonyl CoA. |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-モデル
モデル #143 | タイプ: dummy / ソフトウェア: Sasref / ダミー原子の半径: 1.90 A / カイ2乗値: 24.01 Omokage検索でこの集合体の類似形状データを探す (詳細) |
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-試料
試料 | 名称: Endophilin-CoA complex / Sample MW: 118 kDa / 試料濃度: 1 mg/ml / Entity id: 105 / 107 |
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バッファ | 名称: TRIS-HCL / 濃度: 50.00 mM / pH: 8 / 組成: NaCl 300.000 mM |
要素 #105 | 名称: Endophilin / タイプ: protein / 記述: Endophilin-A1 BAR domain / 分子量: 29 / 分子数: 2 / 由来: Mus musculus 配列: MSVAGLKKQF HKATQKVSEK VGGAEGTKLD DDFKEMERKV DVTSRAVMEI MTKTIEYLQP NPASRAKLSM INTMSKIRGQ EKGPGYPQAE ALLAEAMLKF GRELGDDCNF GPALGEVGEA MRELSEVKDS LDMEVKQNFI DPLQNLHDKD LREIQHHLKK LEGRRLDFDY ...配列: MSVAGLKKQF HKATQKVSEK VGGAEGTKLD DDFKEMERKV DVTSRAVMEI MTKTIEYLQP NPASRAKLSM INTMSKIRGQ EKGPGYPQAE ALLAEAMLKF GRELGDDCNF GPALGEVGEA MRELSEVKDS LDMEVKQNFI DPLQNLHDKD LREIQHHLKK LEGRRLDFDY KKKRQGKIPD EELRQALEKF DESKEIAESS MFNLLEMDIE QVSQLSALVQ AQLEYHKQAV QILQQVTVRL EERIRQA |
要素 #107 | 名称: CoA / タイプ: other / 記述: arachidonyl-CoA |
-実験情報
ビーム | 設備名称: DORIS III X33 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron | |||||||||
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検出器 | 名称: MAR 345 Image Plate | |||||||||
スキャン | タイトル: complex / 測定日: 2006年11月27日 / 単位: 1/nm /
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結果 | I0 from Guinier: 435.06 / Rg from Guinier: 5.9 nm / D max: 19 / カーブのタイプ: single_conc /
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