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- SASDAX5: Endophilin-CoA complex (Endophilin-A1 BAR domain, Endophilin + ar... -

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Basic information

Entry
Database: SASBDB / ID: SASDAX5
SampleEndophilin-CoA complex
  • Endophilin-A1 BAR domain (protein), Endophilin, Mus musculus
  • arachidonyl-CoA (other), CoA
Biological speciesMus musculus (house mouse)
CitationJournal: Front Mol Biosci / Year: 2014
Title: Endophilin-A1 BAR domain interaction with arachidonyl CoA.
Authors: Maxim V Petoukhov / Winfried Weissenhorn / Dmitri I Svergun /
Abstract: Endophilin-A1 belongs to the family of BAR domain containing proteins that catalyze membrane remodeling processes via sensing, inducing and stabilizing membrane curvature. We show that the BAR domain ...Endophilin-A1 belongs to the family of BAR domain containing proteins that catalyze membrane remodeling processes via sensing, inducing and stabilizing membrane curvature. We show that the BAR domain of endophilin-A1 binds arachidonic acid and molds its coenzyme A (CoA) activated form, arachidonyl-CoA into a defined structure. We studied low resolution structures of endophilin-A1-BAR and its complex with arachidonyl-CoA in solution using synchrotron small-angle X-ray scattering (SAXS). The free endophilin-A1-BAR domain is shown to be dimeric at lower concentrations but builds tetramers and higher order complexes with increasing concentrations. Extensive titration SAXS studies revealed that the BAR domain produces a homogenous complex with the lipid micelles. The structural model of the complexes revealed two arachidonyl-CoA micelles bound to the distal arms of an endophilin-A1-BAR dimer. Intriguingly, the radius of the bound micelles significantly decreases compared to that of the free micelles, and this structural result may provide hints on the potential biological relevance of the endophilin-A1-BAR interaction with arachidonyl CoA.
Contact author
  • Maxim Petoukhov (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

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Models

Model #143
Type: dummy / Software: Sasref / Radius of dummy atoms: 1.90 A / Chi-square value: 24.01
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Endophilin-CoA complex / Sample MW: 118 kDa / Specimen concentration: 1 mg/ml / Entity id: 105 / 107
BufferName: TRIS-HCLTris / Concentration: 50.00 mM / pH: 8 / Composition: NaCl 300.000 mM
Entity #105Name: Endophilin / Type: protein / Description: Endophilin-A1 BAR domain / Formula weight: 29 / Num. of mol.: 2 / Source: Mus musculus
Sequence: MSVAGLKKQF HKATQKVSEK VGGAEGTKLD DDFKEMERKV DVTSRAVMEI MTKTIEYLQP NPASRAKLSM INTMSKIRGQ EKGPGYPQAE ALLAEAMLKF GRELGDDCNF GPALGEVGEA MRELSEVKDS LDMEVKQNFI DPLQNLHDKD LREIQHHLKK LEGRRLDFDY ...Sequence:
MSVAGLKKQF HKATQKVSEK VGGAEGTKLD DDFKEMERKV DVTSRAVMEI MTKTIEYLQP NPASRAKLSM INTMSKIRGQ EKGPGYPQAE ALLAEAMLKF GRELGDDCNF GPALGEVGEA MRELSEVKDS LDMEVKQNFI DPLQNLHDKD LREIQHHLKK LEGRRLDFDY KKKRQGKIPD EELRQALEKF DESKEIAESS MFNLLEMDIE QVSQLSALVQ AQLEYHKQAV QILQQVTVRL EERIRQA
Entity #107Name: CoA / Type: other / Description: arachidonyl-CoA

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Experimental information

BeamInstrument name: DORIS III X33 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron
DetectorName: MAR 345 Image Plate
Scan
Title: complex / Measurement date: Nov 27, 2006 / Unit: 1/nm /
MinMax
Q0.1465 4.991
ResultI0 from Guinier: 435.06 / Rg from Guinier: 5.9 nm / D max: 19 / Type of curve: single_conc /
ExperimentalPorod
MW130 kDa-
Volume-480 nm3

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