SASDAS7: mouse olfactomedin-1 (Noelin, Olfactomedin-1)

基本情報

• 登録情報: データベース: SASBDB / ID: SASDAS7

試料

mouse olfactomedin-1

• Noelin (protein), Olfactomedin-1, Mus musculus

機能・相同性

分子機能:

regulation of amyloid-beta formation / atrioventricular valve formation / neuronal signal transduction / extrinsic component of synaptic membrane / cardiac epithelial to mesenchymal transition / regulation of axon extension / extrinsic component of postsynaptic density membrane / negative regulation of amyloid-beta formation / AMPA glutamate receptor complex / axonal growth cone / positive regulation of epithelial to mesenchymal transition / synaptic membrane / amyloid-beta binding / perikaryon / positive regulation of apoptotic process / axon / negative regulation of gene expression / neuronal cell body / positive regulation of gene expression / glutamatergic synapse / endoplasmic reticulum / extracellular space / identical protein binding

ドメイン・相同性:

Noelin domain / Neurogenesis glycoprotein / : / Olfactomedin-like domain / Olfactomedin-like domain / Olfactomedin-like domain profile. / Olfactomedin-like domains / Quinoprotein amine dehydrogenase, beta chain-like / Endoplasmic reticulum targeting sequence.

構成要素:

Noelin

• 生物種: Mus musculus (ハツカネズミ)
• 引用: ジャーナル: J Biol Chem / 年: 2015; タイトル: Olfactomedin-1 Has a V-shaped Disulfide-linked Tetrameric Structure.; 著者: Matti F Pronker / Trusanne G A A Bos / Thomas H Sharp / Dominique M E Thies-Weesie / Bert J C Janssen / ; 要旨: Olfactomedin-1 (Olfm1; also known as noelin and pancortin) is a member of the olfactomedin domain-containing superfamily and a highly expressed neuronal glycoprotein important for nervous system development. It binds a number of secreted proteins and cell surface-bound receptors to induce cell signaling processes. Using a combined approach of x-ray crystallography, solution scattering, analytical ultracentrifugation, and electron microscopy we determined that full-length Olfm1 forms disulfide-linked tetramers with a distinctive V-shaped architecture. The base of the "V" is formed by two disulfide-linked dimeric N-terminal domains. Each of the two V legs consists of a parallel dimeric disulfide-linked coiled coil with a C-terminal β-propeller dimer at the tips. This agrees with our crystal structure of a C-terminal coiled-coil segment and β-propeller combination (Olfm1(coil-Olf)) that reveals a disulfide-linked dimeric arrangement with the β-propeller top faces in an outward exposed orientation. Similar to its family member myocilin, Olfm1 is stabilized by calcium. The dimer-of-dimers architecture suggests a role for Olfm1 in clustering receptors to regulate signaling and sheds light on the conformation of several other olfactomedin domain family members.

登録者

• Matti Pronker (Utrecht University, Utrecht, Netherlands)

モデル

• モデル #298: ; タイプ: dummy / ソフトウェア: Dammif / ダミー原子の半径: 6.30 A / カイ2乗値: 4.313; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: mouse olfactomedin-1 / 試料濃度: 3.55 mg/ml
• バッファ: 名称: 20 mM HEPES 150 mM NaCl / 濃度: 20.00 mM / pH: 7.5 / 組成: 150 mM NaCl

要素 #163

名称: Olfactomedin-1 / タイプ: protein / 記述: Noelin / 分子量: 64 / 分子数: 4 / 由来: Mus musculus / 参照: UniProt: O88998
配列:

MITNWMSQTL PSLVGLNTTR LSAASGGTLD RSTGVLPTNP EESWQVYSSA QDSEGRCICT VVAPQQTMCS RDARTKQLRQ LLEKVQNMSQ SIEVLDRRTQ RDLQYVEKME NQMKGLETKF KQVEESHKQH LARQFKAIKA KMDELRPLIP VLEEYKADAK LVLQFKEEVQ NLTSVLNELQ EEIGAYDYDE LQSRVSNLEE RLRACMQKLA CGKLTGISDP VTVKTSGSRF GSWMTDPLAP EGDNRVWYMD GYHNNRFVRE YKSMVDFMNT DNFTSHRLPH PWSGTGQVVY NGSIYFNKFQ SHIIIRFDLK TETILKTRSL DYAGYNNMYH YAWGGHSDID LMVDENGLWA VYATNQNAGN IVISKLDPVS LQILQTWNTS YPKRSAGEAF IICGTLYVTN GYSGGTKVHY AYQTNASTYE YIDIPFQNKY SHISMLDYNP KDRALYAW

実験情報

• ビーム: 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotron / 波長: 0.93 Å / スペクトロメータ・検出器間距離: 2.43 mm
• 検出器: 名称: Pilatus 1M

スキャン

タイトル: mouse Olfactomedin-1 / 測定日: 2013年11月6日 / 保管温度: 20 °C / セル温度: 20 °C / 照射時間: 0.056 sec. / フレーム数: 18 / 単位: 1/nm /

	Min	Max
Q	0.028	4.4936

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.5a / ポイント数: 205 /

	Min	Max
Q	0.05819	0.9375
P(R) point	8	212
R	0	29.86

結果

D max: 30 / カーブのタイプ: single_conc
コメント: SAXS was used to determine that Olfactomedin-1 forms tetramers in solution (confirmed by SEC-MALS and AUC). Scattering data were combined with crystallographic and negative stain electron tomography data to construct a model of a V-shaped dimer-of-dimers.

	実験値	Porod
分子量	249 kDa	323 kDa
体積	-	616 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	250.9	0.2	249	0.3
慣性半径, Rg	8.79 nm	0.008	8.5 nm	-