SASDAG8: Human Filamin A Ig-like domains 20-21* truncation (2141-2329) in ...

基本情報

登録情報

データベース: SASBDB / ID: SASDAG8

試料

Human Filamin A Ig-like domains 20-21* truncation (2141-2329) in complex with migfilin peptide

• Human Filamin A Ig-like domains 20-21*/migfilin peptide complex (protein), FilaminA*/migfilin, Homo sapiens

機能・相同性

分子機能:

regulation of membrane repolarization during atrial cardiac muscle cell action potential / regulation of membrane repolarization during cardiac muscle cell action potential / establishment of Sertoli cell barrier / Myb complex / glycoprotein Ib-IX-V complex / formation of radial glial scaffolds / adenylate cyclase-inhibiting dopamine receptor signaling pathway / positive regulation of integrin-mediated signaling pathway / cytoplasmic sequestering of protein / tubulin deacetylation / actin crosslink formation / blood coagulation, intrinsic pathway / protein localization to bicellular tight junction / apical dendrite / OAS antiviral response / positive regulation of actin filament bundle assembly / positive regulation of neuron migration / positive regulation of potassium ion transmembrane transport / Cell-extracellular matrix interactions / early endosome to late endosome transport / Fc-gamma receptor I complex binding / cell-cell junction organization / positive regulation of neural precursor cell proliferation / positive regulation of platelet activation / protein localization to cell surface / podosome / wound healing, spreading of cells / negative regulation of transcription by RNA polymerase I / megakaryocyte development / receptor clustering / GP1b-IX-V activation signalling / cortical cytoskeleton / positive regulation of axon regeneration / SMAD binding / actin filament bundle / RHO GTPases activate PAKs / brush border / semaphorin-plexin signaling pathway / epithelial to mesenchymal transition / mitotic spindle assembly / cilium assembly / blood vessel remodeling / potassium channel regulator activity / axonal growth cone / heart morphogenesis / positive regulation of substrate adhesion-dependent cell spreading / release of sequestered calcium ion into cytosol / regulation of cell migration / dendritic shaft / protein localization to plasma membrane / actin filament / G protein-coupled receptor binding / protein kinase C binding / synapse organization / mRNA transcription by RNA polymerase II / trans-Golgi network / establishment of protein localization / negative regulation of DNA-binding transcription factor activity / negative regulation of protein catabolic process / kinase binding / cerebral cortex development / platelet aggregation / Z disc / small GTPase binding / positive regulation of protein import into nucleus / actin filament binding / cell-cell junction / actin cytoskeleton / negative regulation of neuron projection development / Platelet degranulation / GTPase binding / actin cytoskeleton organization / angiogenesis / perikaryon / positive regulation of canonical NF-kappaB signal transduction / DNA-binding transcription factor binding / postsynapse / transmembrane transporter binding / protein stabilization / cadherin binding / focal adhesion / glutamatergic synapse / negative regulation of apoptotic process / nucleolus / perinuclear region of cytoplasm / protein homodimerization activity / RNA binding / extracellular exosome / extracellular region / membrane / nucleus / plasma membrane / cytosol / cytoplasm

ドメイン・相同性:

Filamin family / Filamin/ABP280 repeat / Filamin-type immunoglobulin domains / Filamin/ABP280 repeat / Filamin/ABP280 repeat profile. / Filamin/ABP280 repeat-like / Actinin-type actin-binding domain signature 1. / Actinin-type actin-binding domain signature 2. / Actinin-type actin-binding domain, conserved site / Calponin homology domain / Calponin homology (CH) domain / Calponin homology domain / CH domain superfamily / Calponin homology (CH) domain profile. / Immunoglobulin E-set / Immunoglobulin-like fold

構成要素:

Filamin-A

• 生物種: Homo sapiens (ヒト)
• 引用: ジャーナル: PLoS One / 年: 2015; タイトル: Flexible Structure of Peptide-Bound Filamin A Mechanosensor Domain Pair 20-21.; 著者: Jonne Seppälä / Helena Tossavainen / Nebojsa Rodic / Perttu Permi / Ulla Pentikäinen / Jari Ylänne / ; 要旨: Filamins (FLNs) are large, multidomain actin cross-linking proteins with diverse functions. Besides regulating the actin cytoskeleton, they serve as important links between the extracellular matrix and the cytoskeleton by binding cell surface receptors, functioning as scaffolds for signaling proteins, and binding several other cytoskeletal proteins that regulate cell adhesion dynamics. Structurally, FLNs are formed of an amino terminal actin-binding domain followed by 24 immunoglobulin-like domains (IgFLNs). Recent studies have demonstrated that myosin-mediated contractile forces can reveal hidden protein binding sites in the domain pairs IgFLNa18-19 and 20-21, enabling FLNs to transduce mechanical signals in cells. The atomic structures of these mechanosensor domain pairs in the resting state are known, as well as the structures of individual IgFLN21 with ligand peptides. However, little experimental data is available on how interacting protein binding deforms the domain pair structures. Here, using small-angle x-ray scattering-based modelling, x-ray crystallography, and NMR, we show that the adaptor protein migfilin-derived peptide-bound structure of IgFLNa20-21 is flexible and adopts distinctive conformations depending on the presence or absence of the interacting peptide. The conformational changes reported here may be common for all peptides and may play a role in the mechanosensor function of the site.

登録者

• Jonne Seppälä (University of Jyväskylä, Jyväskylän, Finland)

モデル

試料

• 試料: 名称: Human Filamin A Ig-like domains 20-21* truncation (2141-2329) in complex with migfilin peptide; 試料濃度: 1.00-4.00
• バッファ: 名称: Tris / 濃度: 20.00 mM / pH: 8 / 組成: 50 mM NaCl, 10mM DTT

要素 #189

名称: FilaminA*/migfilin / タイプ: protein
記述: Human Filamin A Ig-like domains 20-21*/migfilin peptide complex
分子量: 22.6 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: P21333
配列:

KESITRRRRA PSVANVGSHC DLSLKIPEIS IQDMTAQVTS PSGKTHEAEI VEGENHTYCI RFVPAEMGTH TVSVKYKGQH VPGSPFQFTV GPLGEGGAHK VRAGGPGLER AEAGVPAEFS IWTREAGAGG LAIAVEGPSK AEISFEDRKD GSCGVAYVVQ EPGDYEVSVK FNEEHIPDSP FVVPVASPS

実験情報

• ビーム: 設備名称: ESRF BM29 / 地域: Grenoble / 国: France / 線源: X-ray synchrotron / 波長: 0.93 Å / スペクトロメータ・検出器間距離: 2.43 mm
• 検出器: 名称: Pilatus 1M

スキャン

タイトル: Human Filamin A domains 20-21*/migfilin peptide
測定日: 2014年2月1日 / 保管温度: 20 °C / 照射時間: 1 sec. / フレーム数: 10 / 単位: 1/nm /

	Min	Max
Q	0.0889	4.65

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.6 / ポイント数: 468 /

	Min	Max
Q	0.243	2.49
P(R) point	33	500
R	0	8.19

結果

カーブのタイプ: merged
コメント: Filamin A fragment residues 2141-2329 (Ig-like domains) in complex with migfilin peptide. NOTE: The displayed SAXS data and data used for P(r) vs r determination are on different relative I(s) scales.

	実験値	Porod
分子量	30 kDa	19.4 kDa
体積	-	32.9 nm3

	P(R)	Guinier	P(R) error
前方散乱 I0	30.1	35.8	-
慣性半径, Rg	2.41 nm	2.35 nm	0.004

	Min	Max
D	-	8.2
Guinier point	31	83