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基本情報
登録情報 | ![]() |
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![]() | Antiapoptotic membrane protein, (DpV84gp022) Deerpox virus
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機能・相同性 | Poxvirus F1/C10 / Apoptosis regulator M11L like / symbiont-mediated suppression of host apoptosis / Bcl-2-like superfamily / regulation of apoptotic process / membrane / Antiapoptotic membrane protein![]() |
生物種 | ![]() |
![]() | ![]() タイトル: Structural basis of Deerpox virus-mediated inhibition of apoptosis. 著者: Denis R Burton / Sofia Caria / Bevan Marshall / Michele Barry / Marc Kvansakul / ![]() ![]() 要旨: Apoptosis is a key innate defence mechanism to eliminate virally infected cells. To counteract premature host-cell apoptosis, poxviruses have evolved numerous molecular strategies, including the use ...Apoptosis is a key innate defence mechanism to eliminate virally infected cells. To counteract premature host-cell apoptosis, poxviruses have evolved numerous molecular strategies, including the use of Bcl-2 proteins, to ensure their own survival. Here, it is reported that the Deerpox virus inhibitor of apoptosis, DPV022, only engages a highly restricted set of death-inducing Bcl-2 proteins, including Bim, Bax and Bak, with modest affinities. Structural analysis reveals that DPV022 adopts a Bcl-2 fold with a dimeric domain-swapped topology and binds pro-death Bcl-2 proteins via two conserved ligand-binding grooves found on opposite sides of the dimer. Structures of DPV022 bound to Bim, Bak and Bax BH3 domains reveal that a partial obstruction of the binding groove is likely to be responsible for the modest affinities of DPV022 for BH3 domains. These findings reveal that domain-swapped dimeric Bcl-2 folds are not unusual and may be found more widely in viruses. Furthermore, the modest affinities of DPV022 for pro-death Bcl-2 proteins suggest that two distinct classes of anti-apoptotic viral Bcl-2 proteins exist: those that are monomeric and tightly bind a range of death-inducing Bcl-2 proteins, and others such as DPV022 that are dimeric and only bind a very limited number of death-inducing Bcl-2 proteins with modest affinities. |
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-関連構造データ
関連構造データ | ![]() 4uf1C ![]() 4uf2C ![]() 4uf3C C: 同じ文献を引用 ( |
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-モデル
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試料
![]() | 名称: Antiapoptotic membrane protein, (DpV84gp022) Deerpox virus 試料濃度: 0.22-6.34 |
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バッファ | 名称: HEPES / 濃度: 25.00 mM / pH: 7.5 / 組成: 150 mM NaCl |
要素 #186 | タイプ: protein / 記述: Antiapoptotic membrane protein / 分子量: 19.6 / 分子数: 2 / 由来: Deerpox virus W-1170-84 / 参照: UniProt: Q08FF8 配列: MGSSHHHHHH SQDMEAAIEF DEIVKKLLNI YINDICTTGE KRLLNNYEKS ILDRIYKSCE YIKKNYELDF NSMYNQININ DITTSDIKSK IIEALLIDSR PSVKLATLSF ISLIAEKWGE KNRAKIMEIL SNEIVEKISN NGKDFIDFID RDDDDIVDDY VLITNYLK |
-実験情報
ビーム | 設備名称: Australian Synchrotron SAXS/WAXS / 地域: Melbourne / 国: Australia ![]() | ||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 1M | ||||||||||||||||||||||||||||||
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