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- SASDA98: Thyroglobulin in Tris/HCl (Thyroglobulin) -

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Entry
Database: SASBDB / ID: SASDA98
SampleThyroglobulin in Tris/HCl
Thyroglobulin (protein), Bos taurus
SourceBos taurus / mammal / ウシ /
Citation

, 2007
Accuracy of molecular mass determination of proteins in solution by small-angle X-ray scattering
Array

Contact authorEfstratios Mylonas (EMBL-Hamburg, FORTH, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Foundation for Research and Technology-Hellas, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany, Heraklion, Greece)

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Models

Model #316
Type: dummy / Software: DAMMIN / Radius of dummy atoms: 6.10 A / Chi-square value: 16.507969
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Thyroglobulin in Tris/HCl / Specimen concentration: 3.9
BufferName: Tris/HCl / Concentration: 1 / Unit: mM / pH: 7.5 / Composition: 100 mM NaCl
Entity #182Type: protein / Description: Thyroglobulin / Formula weight: 303.218 / Number of molecules: 2 / Source: Bos taurus / References: UniProt: P01267
Sequence:
MALALWVFGL LDLICLASAN IFEYQVDAQP LRPCELQRER AFLKREDYVP QCAEDGSFQT VQCGKDGASC WCVDADGREV PGSRQPGRPA ACLSFCQLQK QQILLSSYIN STATSYLPQC QDSGDYSPVQ CDLRRRQCWC VDAEGMEVYG TRQQGRPARC PRSCEIRNRR LLHGVGDRSP PQCSPDGAFR PVQCKLVNTT DMMIFDLVHS YSRFPDAFVT FSSFRSRFPE VSGYCYCADS QGRELAETGL ELLLDEIYDT IFAGLDLAST FAETTLYRIL QRRFLAVQLV ISGRFRCPTK CEVERFAATS FRHPYVPSCH PDGEYQAAQC QQGGPCWCVD SRGQEIPGTR QRGEPPSCAE DQSCPSERRR AFSRLRFGPS GYFSRRSLLL APEEGPVSQR FARFTASCPP SIKELFLDSG IFQPMLQGRD TRFVAPESLK EAIRGLFPSR ELARLALQFT TNAKRLQQNL FGGRFLVKVG QFNLSGALGT RGTFNFSHFF QQLGLPGFQD GRALADLAKP LSVGLNSNPA SEAPKASKID VALRKPVVGS FGFEVNLQEN QNALQFLSSF LELPEFLLFL QHAISVPEDI ARDLGDVMEM VFSSQGCGQA PGSLFVPACT AEGSYEEVQC FAGDCWCVDA QGRELAGSRV RGGRPRCPTE CEKQRARMQS LLGSQPAGSS LFVPACTSKG NFLPVQCFNS ECYCVDTEGQ PIPGTRSALG EPKKCPSPCQ LQAERAFLGT VRTLVSNPST LPALSSIYIP QCSASGQWSP VQCDGPPEQA FEWYERWEAQ NSAGQALTPA ELLMKIMSYR EAASRNFRLF IQNLYEAGQQ GIFPGLARYS SFQDVPVSVL EGNQTQPGGN VFLEPYLFWQ ILNGQLDRYP GPYSDFSAPL AHFDLRSCWC VDEAGQKLEG TRNEPNKVPA CPGSCEEVKL RVLQFIREAE EIVTYSNSSR FPLGESFLAA KGIRLTDEEL AFPPLSPSRE TFLEKFLSGS DYAIRLAAQS TFDFYQRRLV TLAESPRAPS PVWSSAYLPQ CDAFGGWEPV QCHAATGHCW CVDGKGEYVP TSLTARSRQI PQCPTSCERL RASGLLSSWK QAGVQAEPSP KDLFIPTCLE TGEFARLQAS EAGTWCVDPA SGEGVPPGTN SSAQCPSLCE VLQSGVPSRR TSPGYSPACR AEDGGFSPVQ CDPAQGSCWC VLGSGEEVPG TRVAGSQPAC ESPQCPLPFS VADVAGGAIL CERASGLGAA AGQRCQLRCS QGYRSAFPPE PLLCSVQRRR WESRPPQPRA CQRPQFWQTL QTQAQFQLLL PLGKVCSADY SGLLLAFQVF LLDELTARGF CQIQVKTAGT PVSIPVCDDS SVKVECLSRE RLGVNITWKL QLVDAPPASL PDLQDVEEAL AGKYLAGRFA DLIQSGTFQL HLDSKTFSAD TSIRFLQGDR FGTSPRTQFG CLEGFGRVVA ASDASQDALG CVKCPEGSYF QDEQCIPCPA GFYQEQAGSL ACVPCPEGRT TVYAGAFSQT HCVTDCQKNE VGLQCDQDSQ YRASQRDRTS GKAFCVDGEG RRLPWTEAEA PLVDAQCLVM RKFEKLPESK VIFSADVAVM VRSEVPGSES SLMQCLADCA LDEACGFLTV STAGSEVSCD FYAWASDSIA CTTSGRSEDA LGTSQATSFG SLQCQVKVRS REGDPLAVYL KKGQEFTITG QKRFEQTGFQ SALSGMYSPV TFSASGASLA EVHLFCLLAC DHDSCCDGFI LVQVQGGPLL CGLLSSPDVL LCHVRDWRDP AEAQANASCP GVTYDQDSRQ VTLRLGGQEI RGLTPLEGTQ DTLTSFQQVY LWKDSDMGSR SESMGCRRDT EPRPASPSET DLTTGLFSPV DLIQVIVDGN VSLPSQQHWL FKHLFSLQQA NLWCLSRCAG EPSFCQLAEV TDSEPLYFTC TLYPEAQVCD DILESSPKGC RLILPRRPSA LYRKKVVLQD RVKNFYNRLP FQKLTGISIR NKVPMSDKSI SSGFFECERL CDMDPCCTGF GFLNVSQLKG GEVTCLTLNS LGLQTCSEEY GGVWRILDCG SPDTEVRTYP FGWYQKPVSP SDAPSFCPSV ALPALTENVA LDSWQSLALS SVIVDPSIRN FDVAHISTAA VGNFSAARDR CLWECSRHQD CLVTTLQTQP GAVRCMFYAD TQSCTHSLQA QNCRLLLHEE ATYIYRKPNI PLPGFGTSSP SVPIATHGQL LGRSQAIQVG TSWKPVDQFL GVPYAAPPLG EKRFRAPEHL NWTGSWEATK PRARCWQPGI RTPTPPGVSE DCLYLNVFVP QNMAPNASVL VFFHNAAEGK GSGDRPAVDG SFLAAVGNLI VVTASYRTGI FGFLSSGSSE LSGNWGLLDQ VVALTWVQTH IQAFGGDPRR VTLAADRGGA DIASIHLVTT RAANSRLFRR AVLMGGSALS PAAVIRPERA RQQAAALAKE VGCPSSSVQE MVSCLRQEPA RILNDAQTKL LAVSGPFHYW GPVVDGQYLR ETPARVLQRA PRVKVDLLIG SSQDDGLINR AKAVKQFEES QGRTSSKTAF YQALQNSLGG EAADAGVQAA ATWYYSLEHD SDDYASFSRA LEQATRDYFI ICPVIDMASH WARTVRGNVF MYHAPESYSH SSLELLTDVL YAFGLPFYPA YEGQFTLEEK SLSLKIMQYF SNFIRSGNPN YPHEFSRRAP EFAAPWPDFV PRDGAESYKE LSVLLPNRQG LKKADCSFWS KYIQSLKASA DETKDGPSAD SEEEDQPAGS GLTEDLLGLP ELASKTYSK

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Experimental information

BeamInstrument name: DORIS III X33 / Instrument city: Hamburg / Instrument country: Germany / Type of source: X-ray synchrotron
ScanTitle: Thyroglobulin / Measurement date: May 19, 2006 / Cell temperature: 2 / Unit: 1/nm / Qmin: 0.097 / Qmax: 4.9563
Distance distribution function P(R)Software p of R: GNOM 4.5a / Number of points: 512 / Qmin: 0.121 / Qmax: 4.791 / P of R point min: 10 / P of R point max: 521 / Rmin: 0 / Rmax: 24
ResultSASBDB code: SASDA98 / Experimental MW: 622 / MW standard: 622 / I0 from PR: 2331 / I0 from Guinier: 2510.92 / Rg from PR: 7.41 / Rg from Guinier: 7.86 / D max: 24 / Guinier point min: 9 / Guinier point max: 30 / Type of curve: single_conc
Comments: Thyroglobulin contains glycans and extra iodines, for these reasons the molecular weight based on the sequence (606 kDa) does not agree with the experimentally calculated one (622 kDa).

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