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- PDB-9m08: Structure of outer membrane lipoprotein QseG and histidine kinase... -

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Basic information

Entry
Database: PDB / ID: 9m08
TitleStructure of outer membrane lipoprotein QseG and histidine kinase QseE complex
Components
  • Quorum-sensing regulator protein G
  • histidine kinase
KeywordsSIGNALING PROTEIN / complex
Function / homology
Function and homology information


osmosensory signaling via phosphorelay pathway / phosphorelay response regulator activity / phosphorelay sensor kinase activity / histidine kinase / protein kinase activator activity / ATP binding / plasma membrane
Similarity search - Function
Quorum-sensing regulator protein G / YfhG lipoprotein / : / Histidine kinase/HSP90-like ATPase / His Kinase A (phospho-acceptor) domain / His Kinase A (phosphoacceptor) domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain / Signal transduction histidine kinase-related protein, C-terminal / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily / Histidine kinase domain ...Quorum-sensing regulator protein G / YfhG lipoprotein / : / Histidine kinase/HSP90-like ATPase / His Kinase A (phospho-acceptor) domain / His Kinase A (phosphoacceptor) domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain / Signal transduction histidine kinase-related protein, C-terminal / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily / Histidine kinase domain / Histidine kinase domain profile. / Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase / Histidine kinase-like ATPases / Histidine kinase/HSP90-like ATPase superfamily
Similarity search - Domain/homology
Hypothetical membrane protein / histidine kinase
Similarity search - Component
Biological speciesSalmonella enterica subsp. enterica serovar Typhimurium str. SL1344 (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.9 Å
AuthorsGao, X. / Li, G.B. / Gong, P.Q.
Funding support China, 1items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32200144 China
CitationJournal: Mbio / Year: 2025
Title: Cryo-EM structure of the QseG-QseE complex reveals an accessory protein-driven two-component system activation mechanism
Authors: Gao, X. / Li, G.B. / Gong, P.Q.
History
DepositionFeb 24, 2025Deposition site: PDBJ / Processing site: PDBC
Revision 1.0Nov 19, 2025Provider: repository / Type: Initial release

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Structure visualization

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Assembly

Deposited unit
A: Quorum-sensing regulator protein G
B: Quorum-sensing regulator protein G
C: histidine kinase
D: histidine kinase


Theoretical massNumber of molelcules
Total (without water)151,5414
Polymers151,5414
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

#1: Protein Quorum-sensing regulator protein G / Two-component system QseEF-associated lipoprotein QseG


Mass: 21022.740 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 (bacteria)
Gene: yfhG, qseG, SL1344_2525, G1W50_19190, G1W53_19070, G1W54_19660, G1W56_19535, G1W63_19335, G1W64_19365, G1W86_18045, G1W87_18985, G1W89_18830, G1W92_18735, G1X03_18695, G1X04_08460, G1X09_19680, ...Gene: yfhG, qseG, SL1344_2525, G1W50_19190, G1W53_19070, G1W54_19660, G1W56_19535, G1W63_19335, G1W64_19365, G1W86_18045, G1W87_18985, G1W89_18830, G1W92_18735, G1X03_18695, G1X04_08460, G1X09_19680, G1X10_19590, G1X13_19670, G1X17_20470, G1X23_19065, G1X29_19380, G1X32_19630, G1X38_19925, G1X42_18740, G1X47_19355, G1X49_18700, G1X51_19680, G1X52_19370, G1X66_19045, G1X68_16400, G1X69_19950, G1X71_19685, G1X76_19340, G1X80_18180, G1X84_18735, G1X85_19495, G1X86_19670, G1X94_19190, G1X96_19675, G1X99_19370, G1Y02_19370
Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A0H3NKH9
#2: Protein histidine kinase


Mass: 54747.652 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 (bacteria)
Gene: G1W50_19185, G1W53_19065, G1W54_19655, G1W56_19530, G1W63_19330, G1W64_19360, G1W86_18040, G1W87_18980, G1W89_18825, G1W92_18730, G1X03_18690, G1X04_08455, G1X09_19675, G1X10_19585, G1X13_ ...Gene: G1W50_19185, G1W53_19065, G1W54_19655, G1W56_19530, G1W63_19330, G1W64_19360, G1W86_18040, G1W87_18980, G1W89_18825, G1W92_18730, G1X03_18690, G1X04_08455, G1X09_19675, G1X10_19585, G1X13_19665, G1X17_20465, G1X23_19060, G1X29_19375, G1X32_19625, G1X38_19920, G1X42_18735, G1X47_19350, G1X49_18695, G1X51_19675, G1X52_19365, G1X66_19040, G1X68_16395, G1X69_19945, G1X71_19680, G1X76_19335, G1X80_18175, G1X84_18730, G1X85_19490, G1X86_19665, G1X94_19185, G1X96_19670, G1X99_19365, G1Y02_19365
Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A718UUF8, histidine kinase
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: QseG58-220 and QseE sensor complex / Type: COMPLEX / Entity ID: all / Source: MULTIPLE SOURCES
Source (natural)Organism: Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 (bacteria)
Source (recombinant)Organism: Escherichia coli BL21(DE3) (bacteria)
Buffer solutionpH: 8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 500 nm
Image recordingElectron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

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Processing

EM softwareName: PHENIX / Version: 1.20.1_4487 / Category: model refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.9 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 88834 / Symmetry type: POINT
RefinementHighest resolution: 3.9 Å
Stereochemistry target values: REAL-SPACE (WEIGHTED MAP SUM AT ATOM CENTERS)

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