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- PDB-9bkk: Cholecystokinin 1 receptor (CCK1R) sterol 7M mutant, Gq chimera (... -
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Open data
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Basic information
Entry | Database: PDB / ID: 9bkk | |||||||||||||||
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Title | Cholecystokinin 1 receptor (CCK1R) sterol 7M mutant, Gq chimera (mGsqi) complex | |||||||||||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||||||||
Biological species | ![]() ![]() | |||||||||||||||
Method | ![]() ![]() ![]() | |||||||||||||||
![]() | Harikumar, K.G. / Zhao, P. / Cary, B.P. / Xu, X. / Desai, A.J. / Mobbs, J.I. / Toufaily, C. / Furness, S.G.B. / Christopoulos, A. / Belousoff, M.J. ...Harikumar, K.G. / Zhao, P. / Cary, B.P. / Xu, X. / Desai, A.J. / Mobbs, J.I. / Toufaily, C. / Furness, S.G.B. / Christopoulos, A. / Belousoff, M.J. / Wootten, D. / Sexton, P.M. / Miller, L.J. | |||||||||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: Cholesterol-dependent dynamic changes in the conformation of the type 1 cholecystokinin receptor affect ligand binding and G protein coupling Authors: Harikumar, K.G. / Zhao, P. / Cary, B.P. / Xu, X. / Desai, A.J. / Mobbs, J.I. / Toufaily, C. / Furness, S.G.B. / Christopoulos, A. / Belousoff, M.J. / Wootten, D. / Sexton, P.M. / Miller, L.J. | |||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 172.4 KB | Display | ![]() |
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PDB format | ![]() | 129.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 44643MC ![]() 9bkjC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 29144.971 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,with certain residues mutated to match Guanine nucleotide-binding protein G(q) subunit Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 37413.863 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#3: Protein | Mass: 7861.143 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#4: Protein/peptide | ![]() Mass: 1142.262 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() ![]() |
#5: Protein | Mass: 47694.750 Da / Num. of mol.: 1 / Mutation: F130L, S136A, G141S, I216L, L219F, I223V, M226A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: ![]() |
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Sample preparation
Component | Name: Complex of CCK1R (sterol 7M mutant) bound to miniGs (Gq, Gi chimera), G-beta1, G-gamma-2 and CCK8s. Type: COMPLEX Details: Single-chain fragment variable 16 (ScFv16) was included in the complex but left unmodeled. Entity ID: all / Source: RECOMBINANT |
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Molecular weight | Value: 0.15 MDa / Experimental value: NO |
Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 7.4 |
Specimen | Conc.: 7.8 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied![]() ![]() |
Specimen support | Details: Gold coated. Negative polarity glow discharge. / Grid material: GOLD / Grid type: Quantifoil R1.2/1.3 |
Vitrification![]() | Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K |
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Electron microscopy imaging
Microscopy | Model: FEI TITAN |
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Electron gun | Electron source![]() ![]() |
Electron lens | Mode: BRIGHT FIELD![]() |
Specimen holder | Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Image recording | Electron dose: 60 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) / Num. of grids imaged: 1 / Num. of real images: 6878 |
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Processing
EM software |
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CTF correction![]() | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||||||
Particle selection | Num. of particles selected: 3644147 | ||||||||||||||||||||||||||||||||
Symmetry | Point symmetry![]() | ||||||||||||||||||||||||||||||||
3D reconstruction![]() | Resolution: 2.51 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 279231 / Symmetry type: POINT | ||||||||||||||||||||||||||||||||
Atomic model building | PDB-ID: 7MBY Accession code: 7MBY / Source name: PDB / Type: experimental model |